SCIENTIFIC INSTRUMENTATION
INSTRUMENTS MENU
BIOASSAYS
ExperimentELISA[]
Robotic ELISA Device
Absorbance Detection
- LED Light Source
- 8-channel photometer
- 340 to 750 nm wavelength selection
- Linear shaking
- 0.1 mOD resolution
- < ± 1 % precision
Incubation
- Heating from room temperature to 80°C
- Shaking from 0 to 1,800 RPM at 0 to 3 mm orbit
Washing
- 8-channel strip washer
- 50 to 1,000 µL wash buffer per well
- 1 to 9 wash cycles
- Linear sample shaking
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/nimbus-elisa-system.png?resize=150%2C150&ssl=1)
ExperimentSingleCellPreparation[]
Single Cell Instrument
- Characterize complex cell populations
- Multiomic profiling of immune diversity, cell by cell
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/Screen-Shot-2023-05-22-at-3.00.03-PM.png?resize=150%2C126&ssl=1)
ExperimentCapillaryELISA[]
Capillary ELISA Device
- All incubation, washing, and detection steps are automated
- 72 samples with 1 analytes, or 32 samples with up to 8 analytes
- Built-in duplicate or triplicate measurement per sample
- 25 µL volume required per sample
- 4 to 5 logs of dynamic range
- 75 minute run time
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/proteinsimple-ella.png?resize=150%2C150&ssl=1)
ExperimentSequencing[]
Alternative Next Generation Sequencing Machine
- Output Range: 540 Mb – 15 Gb
- Reads per Run: 1-25 million
- Max read length: 2x 300 bp
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/system-carousel-miseq-left.png?resize=150%2C150&ssl=1)
ExperimentCapillaryElectrophoresis[]
Capillary Electrophoresis System
- Sample Trays: 2 x 48 CESI vials / 0.3 mL microvials
- Buffer Tray: 2 x 36 CESI vials
- Pressure Delivery Range: -5 to +100 PSI
- Required Sample Volume: 5 µL when using NanoVias
- Zero Dead Volume
- Multi-Detection Capability: UV/VIS, PDA, or LIF detection can be selected in stand-alone CE mode
- Ultra-low-fow < 10 nLimin, into the mass spectrometer resulting an overall increase in ionization efficiency
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/cesi-8000-1.png?resize=150%2C150&ssl=1)
Tape Station
- Sample number: 1 to 96
- Sample volume: 1 to 2 µL
- Sizing range: 200 to > 60,000 bp
- Sensitivity: 0.5 ng/µL
- Sizing precision: 200 bp to 15,000 bp: 15 %CV
- Sizing accuracy: 200 bp to 15,000 bp: ± 15 %
- Quantitative precision 15 %CV
- Quantitative accuracy: ± 20 %
- Quantitative range: 10 to 100 ng/µL
- DNA Integrity Number functional range: 5 to 300 ng/µL
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/proteinsimple-ella.png?resize=150%2C150&ssl=1)
ExperimentWestern[]
ExperimentTotalProteinDetection[]
Example applications include: Capillary-based Analytical Assay, Gel Electrophoresis, Capillary Gel Electrophoresis, Total Protein Separation, Total Protein Visualization, Protein Size Characterization, Protein Abundance Comparison
Capillary Western Blot System
Capillary Gel Electrophoresis
- 2 to 440 kDa molecular weight range
- 0 to 500 V separation voltage
- UV capillary crosslinking for protein immobilization
Secondary Antibodies Available
- Secondary Antibodies Available
- Goat Anti-Rabbit HRP
- Goat Anti-Mouse HRP
- Goat Anti-Human IgG HRP
- Donkey Anti-Goat HRP
CCD Camera-Based Capillary Imaging
- 100 ms to 512 sec exposure times
- Up to picogram sensitivity (depending on the primary antibody sensitivity)
Capacity
- 13 or 25 capillaries per batch
- 5 to 8 µL of sample required per capillary
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/proteinsimple-wes.png?resize=150%2C150&ssl=1)
ExperimentqPCR[]
Example applications include: Gene Expression Analysis, Genotyping, Copy Number Variation Analysis, microRNA Analysis, Pathogen Detection
qPCR Thermocycler
Excitation Filters
- 470 nm (15 nm bandwidth)
- 520 nm (10 nm bandwidth)
- 550 nm (10 nm bandwidth)
- 580 nm (10 nm bandwidth)
- 640 nm (10 nm bandwidth)
- 662 nm (10 nm bandwidth)
Emission Filters
- 520 nm (15 nm bandwidth)
- 558 nm (12 nm bandwidth)
- 587 nm (10 nm bandwidth)
- 623 nm (14 nm bandwidth)
- 682 nm (14 nm bandwidth)
- 711 nm (12 nm bandwidth)
Halogen Lamp Reaction Conditions
- 4 to 100°C temperature range
- 204°C/min maximum temperature ramp rate
- Programmable thermal cycle program (up to 100 cycles)
- Reactions in 384-well plates or array cards
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/COL01191-4485701-QuantStudio-7-left-facing.jpg-650.webp?resize=150%2C150&ssl=1)
ExperimentDigitalPCR[]
Digital PCR System
- Up to 480 samples per run
- Absolute quantification of DNA/RNA targets
- Dynamic range: 0.25 to 5,000 targets per μL sample
- Multiplexing with 4 detection channels per sample
- Multiplex up to 8 targets per sample with amplitude multiplexing
Sample Constraints
- 20 μL sample volume
- 96-well plate format
- Fluorescent probe-based detection
Droplet Generation
- In-plate microfluidics for droplet formation
- Up to 20,000 nanoliter-sized droplets per sample
- Droplet generation for 16 wells in parallel
Thermocycling
- 100°C maximum temperature
- 150°C/min maximum temperature ramp rate
- Programmable temperature gradient over columns
- Programmable thermal cycling (Up to 99 cycles)
Droplet Reading
- Sample illumination: LED
- 505 nm (30 nm bandwidth)
- 622 nm (15 nm bandwidth)
- Detector: Multi-pixel Photon Counter
- Emission filters
- 538 nm (25 nm bandwidth)
- 572 nm (40 nm bandwidth)
- 662 nm (40 nm bandwidth)
- 720 nm (60 nm bandwidth)
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/QX-ONE.png?resize=150%2C150&ssl=1)
ExperimentSurfacePlasmonResonance[]
UNDER DEVELOPMENT
Surface Plasmon Resonance System
- Flow cells: 4
- Flow rate range: 1 to 100 µL/min
- Analyte detection range: No lower molecular weight limit for organic molecules
- Association rate constant range (protein): 103 to 3×109 M-1s-1
- Association rate constant range (low molecular weight): 103 to 5×107 M-1s-1
- Dissociation rate constant range: 10-5 to 1 s-1
- Sample temperature range: 4 to 45°C
- Analysis temperature range: 4 to 45ºC
- Sample refractive index range: 1.33 to 1.40
- Automatic switching between 4 buffers
Data Types
- Kinetic and affinity
- Specificity
- Selectivity
- Concentration
- Thermodynamic
Sample Constraints
- Sample input format: 96- or 384-well and up to 33 vials
- Sample injection volume: 2 to 350 µL
- Injection dead volume: 20 to 50 µL
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/ge-biacore.png?resize=150%2C150&ssl=1)
ExperimentBioLayerInterferometry[]
Example applications include: Label-free Assay, Surface Immobilization, Biomolecular Interactions, Concentration Quantification, Association And Dissociation Analysis
Bio-Layer Interferometer (BLI)
Instrument Details
- 8 parallel channels per read
- Sample temperature range: 15 to 40°C in 1°C increments
- Orbital flow capacity: Static or 100 to 1,500 RPM
- Uses commercially available bio-probes
- Baseline noise: < 3 pm
Experiment Types
- Kinetics
- Measure association and dissociation rates
- Up to 12 samples per plate
- Quantitation
- Measure analyte concentration
- Up to 96 samples per plate
- Epitope binning/cross-blocking
- Determine modes of interaction through analyte competition
- Up to 8 samples per plate
Detection Range
- Analyte molecular weight: > 150 Da
- Affinity range: 1 mM to 10 pM
- Association rate range: 10¹ to 10⁷ M⁻¹s⁻¹
- Dissociation rate range: 10⁻⁶ to 0.1 s⁻¹
Sample Constraints
- Sample input format: 96-well
- Minimum sample volume: 180 μL
- Recoverable samples/non-destructive analysis
- Sample types:
- Proteins/Peptides
- Small molecules/Fragments
- Oligonucleotides/DNA/RNA
- Virus-like particles
- Antibodies
- Serum containing media
- DMSO containing buffers
- Crude cell lysates
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/octet-r8.png?resize=150%2C150&ssl=1)
ExperimentIsothermalTitrationCalorimetry[]
Isothermal Titration Calorimeter
- 190 µL active cell volume
- Up to 250 µL injection volume
- 2 to 80 °C temperature range
- 0.05 to 5,000 µJ measurable heat range
- 0 to 200 RPM stirring
- Automated injection syringe cleaning
- Refrigerated autosampler compatible with 96-well plate
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/ta-affinity.png?resize=150%2C150&ssl=1)
ExperimentUVMelting[]
Example applications include: Thermal melting curves, Nucleic acid melting point measurement
UV/Vis Spectrometer with Thermal Controller
- Simultaneously scan a full wavelength range, on 8 channels, in less than a second
- Each pair of cuvettes can be held at a different temperature: Perform 4 different temperature experiments at the same time
- Simultaneously measure standards, samples, and controls under exactly the same conditions
- Measurement modules do not contain any moving parts (permanent optical alignment)
Absorbance Detection
- Photometric system: Double beam with rear beam access
- Long-life xenon flashlamp source
- 250 points per second data collection rate
- 190 to 1,100 nm wavelength range (limiting resolution 0.1 nm, wavelength accuracy ± 0.2 nm)
- 150,000 nm/min maximum scan rate
- Tightly-controlled beam geometry (< 1.5 mm at sample interface)
- Silicon photodiode detectors for simultaneous measurement of all channels
- 8 cuvette positions can be measured simultaneously
Thermodynamics
- Temperature control system is self-contained, integrated, and air-cooled
- Water-free temperature cycling from 0 to 110°C
- Peltier block probe ± 0.5°C temperature accuracy
- Sample probe ± 0.25ºC temperature accuracy
- < ± 0.15°C cell to cell variation
- Maximum ramp rate 40°C/min
- Minimum ramp rate 0.1°C/min
- 1, 2, or 4 independently controlled temperature zones
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/agilent-cary-3500.png?resize=150%2C150&ssl=1)
ExperimentThermalShift[]
Example applications include: Multimode Spectrophotometer, Thermal Stability, Conformation Change Detection, Protein Aggregation Detection, Melting Curve Analysis, Aggregation Curve Analysis
qPCR Thermocycler
Excitation Filters
- 470 nm (15 nm bandwidth)
- 520 nm (10 nm bandwidth)
- 550 nm (10 nm bandwidth)
- 580 nm (10 nm bandwidth)
- 640 nm (10 nm bandwidth)
- 662 nm (10 nm bandwidth)
Emission Filters
- 520 nm (15 nm bandwidth)
- 558 nm (12 nm bandwidth)
- 587 nm (10 nm bandwidth)
- 623 nm (14 nm bandwidth)
- 682 nm (14 nm bandwidth)
- 711 nm (12 nm bandwidth)
Halogen Lamp Reaction Conditions
- 4 to 100°C temperature range
- 204°C/min maximum temperature ramp rate
- Programmable thermal cycle program (up to 100 cycles)
- Reactions in 384-well plates
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/thermo-fisher-viia-7-1.png?resize=150%2C150&ssl=1)
Multimode Spectrophotometer
- Simultaneously measure fluorescence spectra and static light scattering of samples enabling assessment of melting point and aggregation point
- Measurement in sealed quartz capillaries requires only 9 μL of sample
- Measure up to 48 samples in one experiment including standards, samples, and controls
Fluorescence and Static Light Scattering Detection
- 266 nm and 473 nm laser light sources with neutral density filtering available
- CCD spectrometer at full 250 to 750 nm spectral range simultaneously detects fluorescence emission spectra and static light scattering intensity at 266 nm and 473 nm
- Melting point precision < 2% CV
- Static light scattering resolution can detect ~15 kDa change in mean molecular mass
Thermodynamics
- Temperature control system is self-contained
- Water-free temperature cycling from 15 to 95ºC
- TEC heating/cooling plate temperature control accuracy 1ºC (at < 70ºC) and 1.5ºC (at > 70ºC)
- Maximum ramp rate 10ºC/min
- Minimum ramp rate 0.1ºC/min
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/unchained-labs-uncle.png?resize=150%2C150&ssl=1)
ExperimentTotalProteinQuantification[]
Example applications include: Bradford Assay, Bicinchoninic Acid (BCA) Assay, Quant-iT Fluorescence Quantification Assay, Total Protein Quantification, Protein Concentration Quantification
Total Protein Quantification
- Absorbance- and fluorescence-based assays
- Customizable excitation and quantification wavelengths
- Protein quantification between 0.025 and 2 mg/mL
- Between 5 and 25 µL of sample required
- Bovine serum albumin or bovine gamma globulin standards
Instrumentation
- Reagent preparation, plate loading, and mixing performed on Hamilton liquid handler
- Absorbance measured on FLUOstar Omega plate reader
- Fluorescence measured on CLARIOstar plate reader
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/hamilton-star-2.png?resize=150%2C150&ssl=1)
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/fluostar-omega.png?resize=150%2C150&ssl=1)
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/clariostar-1.png?resize=150%2C150&ssl=1)
ExperimentAlphaScreen[]
Example applications include: Bead-based Interaction Assay, Protein-protein Interaction Screening, Binding Inhibitor Screening, Protease/kinase Inhibitor Screening, Binding And Dissociation Analysis
Amplified Luminescent Proximity Homogenous Assay Screen (AlphaScreen)
- Bead-based assay
- AlphaScreen laser and filter sets
- Selection of beads with a variety of affinity tags
- Support 96- or 384-well plate format
- 10 to 160 µL assay volume
Assays Available
- Screen Optimization
- Interaction Characterization
- Interaction Inhibitor Screen
- Interaction Inhibitor Dose Response
- Enzyme Activity Characterization
- Enzyme Inhibitor Screen
- Enzyme Inhibitor Dose Response
- Linker Screen
- Sample Quantification
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/hamilton-star-2.png?resize=150%2C150&ssl=1)
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/clariostar-1.png?resize=150%2C150&ssl=1)
ExperimentBioconjugation[]
Example applications include: Chemical crosslinking, Protein modification, Antibody modification, Fluorophore conjugated, Biotinylated
Liquid Handler
- Low-volume chemical linking of biomolecules including microbeads, oligomers, proteins, and antibodies
- Utilizes all available solid transfer, liquid transfer, and mixing instrumentation
- Biomolecule activation can be specified prior to conjugation.
- Reaction quenching can be specified after conjugation
- Product workup including pelleting and filtering can be specified after conjugation
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/hamilton-star-2.png?resize=150%2C150&ssl=1)
ExperimentBioAnalyzer[]
Fragment Analyzer
- Medium- to high-throughput separation – Analyze 48 or 96 samples in parallel with a single, simple dilution step and easy instrument setup
- Unattended walk-away operation – Load up to three 96-sample microplates, no further user interaction is required
- Reduced sample analysis time – Apply batch processing features post electrophoresis to automatically generate results reports
- Easy adoption to changes in your workflow – Choose a shorter or longer array to maximize your runs regarding speed or resolution of individual samples
- Minimize hands-on time – Reduce the time needed for instrument setup with no daily array handling requirements and room-temperature-stable reagents
- Broad assay portfolio – Analyze samples ranging from gDNA, NGS libraries, and DNA fragments to total RNA, small RNA, and mRNA vaccines all on one platform
- High-resolution separation – Achieve separation resolution as good as 3 bp from fragments under 300 bp
- Objective quality assessment – Qualify your samples using quality metrics for RNA (RQN) and genomic DNA (GQN)
- Seamless switching between applications – Load two different gels into the instrument to use different kits within one system setup
- Comprehensive compliance services – Qualify instruments by IQ/OQ services for use in GMP environments
![](https://i0.wp.com/cloudlab.cmu.edu/wp-content/uploads/2023/05/05_ZD086_M5311AA_5300_Fragment_Analyzer_Ssytem_Closed_F_thumb_1600.webp?resize=150%2C150&ssl=1)