SCIENTIFIC INSTRUMENTATION


BIOASSAYS

ExperimentELISA[]

Robotic ELISA Device

Absorbance Detection

  • LED Light Source
  • 8-channel photometer
  • 340 to 750 nm wavelength selection
  • Linear shaking
  • 0.1 mOD resolution
  • < ± 1 % precision

Incubation

  • Heating from room temperature to 80°C
  • Shaking from 0 to 1,800 RPM at 0 to 3 mm orbit

Washing

  • 8-channel strip washer
  • 50 to 1,000 µL wash buffer per well
  • 1 to 9 wash cycles
  • Linear sample shaking

ExperimentSingleCellPreparation[]

Single Cell Instrument

  • Characterize complex cell populations
  • Multiomic profiling of immune diversity, cell by cell
10x Genomics – Chromium X

ExperimentCapillaryELISA[]

Capillary ELISA Device

  • All incubation, washing, and detection steps are automated
  • 72 samples with 1 analytes, or 32 samples with up to 8 analytes
  • Built-in duplicate or triplicate measurement per sample
  • 25 µL volume required per sample
  • 4 to 5 logs of dynamic range
  • 75 minute run time

ExperimentMultiplexELISA[]

Multiplexing ELISA Screening Device

  • Magnetic bead-based immunoassays
  • Up to 50-analyte multiplexing per sample
  • 35 to 60ºC sample temperature control
  • 3.5 logs of dynamic range
  • 96-well plate assay in less than an hour
  • CCD imager detection

ExperimentSequencing[]

Alternative Next Generation Sequencing Machine

  • Output Range: 540 Mb – 15 Gb
  • Reads per Run: 1-25 million
  • Max read length: 2x 300 bp
Illumina – MiSeq

ExperimentMassivelyParallelSequencing[]

Next Generation Sequencing Machine

  • Compatible with various library preparation kits
  • Mix and match between four fow cell types
  • 532, 660, 780, and 790 nm lasers
  • Highly fexible – outputs between 80 Gb and 800 million reads to 6 Tb and 20 billion reads in less than 2 days

ExperimentCapillaryElectrophoresis[]

Capillary Electrophoresis System

  • Sample Trays: 2 x 48 CESI vials / 0.3 mL microvials
  • Buffer Tray: 2 x 36 CESI vials
  • Pressure Delivery Range: -5 to +100 PSI
  • Required Sample Volume: 5 µL when using NanoVias
  • Zero Dead Volume
  • Multi-Detection Capability: UV/VIS, PDA, or LIF detection can be selected in stand-alone CE mode
  • Ultra-low-fow < 10 nLimin, into the mass spectrometer resulting an overall increase in ionization efficiency

Tape Station

  • Sample number: 1 to 96
  • Sample volume: 1 to 2 µL
  • Sizing range: 200 to > 60,000 bp
  • Sensitivity: 0.5 ng/µL
  • Sizing precision: 200 bp to 15,000 bp: 15 %CV
  • Sizing accuracy: 200 bp to 15,000 bp: ± 15 %
  • Quantitative precision 15 %CV
  • Quantitative accuracy: ± 20 %
  • Quantitative range: 10 to 100 ng/µL
  • DNA Integrity Number functional range: 5 to 300 ng/µL

ExperimentWestern[]

ExperimentTotalProteinDetection[]

Example applications include: Capillary-based Analytical Assay, Gel Electrophoresis, Capillary Gel Electrophoresis, Total Protein Separation, Total Protein Visualization, Protein Size Characterization, Protein Abundance Comparison

Capillary Western Blot System

Capillary Gel Electrophoresis

  • 2 to 440 kDa molecular weight range
  • 0 to 500 V separation voltage
  • UV capillary crosslinking for protein immobilization

Secondary Antibodies Available

  • Secondary Antibodies Available
    • Goat Anti-Rabbit HRP
    • Goat Anti-Mouse HRP
    • Goat Anti-Human IgG HRP
    • Donkey Anti-Goat HRP

CCD Camera-Based Capillary Imaging

  • 100 ms to 512 sec exposure times
  • Up to picogram sensitivity (depending on the primary antibody sensitivity)

Capacity

  • 13 or 25 capillaries per batch
  • 5 to 8 µL of sample required per capillary

ExperimentqPCR[]

Example applications include: Gene Expression Analysis, Genotyping, Copy Number Variation Analysis, microRNA Analysis, Pathogen Detection

qPCR Thermocycler

Excitation Filters

  • 470 nm (15 nm bandwidth)
  • 520 nm (10 nm bandwidth)
  • 550 nm (10 nm bandwidth)
  • 580 nm (10 nm bandwidth)
  • 640 nm (10 nm bandwidth)
  • 662 nm (10 nm bandwidth)

Emission Filters

  • 520 nm (15 nm bandwidth)
  • 558 nm (12 nm bandwidth)
  • 587 nm (10 nm bandwidth)
  • 623 nm (14 nm bandwidth)
  • 682 nm (14 nm bandwidth)
  • 711 nm (12 nm bandwidth)

Halogen Lamp Reaction Conditions

  • 4 to 100°C temperature range
  • 204°C/min maximum temperature ramp rate
  • Programmable thermal cycle program (up to 100 cycles)
  • Reactions in 384-well plates or array cards
ThermoFisher Quantstudio 7

ExperimentDigitalPCR[]

Digital PCR System

  • Up to 480 samples per run
  • Absolute quantification of DNA/RNA targets
  • Dynamic range: 0.25 to 5,000 targets per μL sample
  • Multiplexing with 4 detection channels per sample
  • Multiplex up to 8 targets per sample with amplitude multiplexing

Sample Constraints

  • 20 μL sample volume
  • 96-well plate format
  • Fluorescent probe-based detection

Droplet Generation

  • In-plate microfluidics for droplet formation
  • Up to 20,000 nanoliter-sized droplets per sample
  • Droplet generation for 16 wells in parallel

Thermocycling

  • 100°C maximum temperature
  • 150°C/min maximum temperature ramp rate
  • Programmable temperature gradient over columns
  • Programmable thermal cycling (Up to 99 cycles)

Droplet Reading

  • Sample illumination: LED
    • 505 nm (30 nm bandwidth)
    • 622 nm (15 nm bandwidth)
  • Detector: Multi-pixel Photon Counter
  • Emission filters
    • 538 nm (25 nm bandwidth)
    • 572 nm (40 nm bandwidth)
    • 662 nm (40 nm bandwidth)
    • 720 nm (60 nm bandwidth)
Bio-Rad QXONE

ExperimentSurfacePlasmonResonance[]

Surface Plasmon Resonance System

  • Flow cells: 4
  • Flow rate range: 1 to 100 µL/min
  • Analyte detection range: No lower molecular weight limit for organic molecules
  • Association rate constant range (protein): 103 to 3×109 M-1s-1
  • Association rate constant range (low molecular weight): 103 to 5×107 M-1s-1
  • Dissociation rate constant range: 10-5 to 1 s-1
  • Sample temperature range: 4 to 45°C
  • Analysis temperature range: 4 to 45ºC
  • Sample refractive index range: 1.33 to 1.40
  • Automatic switching between 4 buffers

Data Types

  • Kinetic and affinity
  • Specificity
  • Selectivity
  • Concentration
  • Thermodynamic

Sample Constraints

  • Sample input format: 96- or 384-well and up to 33 vials
  • Sample injection volume: 2 to 350 µL
  • Injection dead volume: 20 to 50 µL

ExperimentBioLayerInterferometry[]

Example applications include: Label-free Assay, Surface Immobilization, Biomolecular Interactions, Concentration Quantification, Association And Dissociation Analysis

Bio-Layer Interferometer (BLI)

Instrument Details

  • 8 parallel channels per read
  • Sample temperature range: 15 to 40°C in 1°C increments
  • Orbital flow capacity: Static or 100 to 1,500 RPM
  • Uses commercially available bio-probes
  • Baseline noise: < 3 pm

Experiment Types

  • Kinetics
    • Measure association and dissociation rates
    • Up to 12 samples per plate
  • Quantitation
    • Measure analyte concentration
    • Up to 96 samples per plate
  • Epitope binning/cross-blocking
    • Determine modes of interaction through analyte competition
    • Up to 8 samples per plate

Detection Range

  • Analyte molecular weight: > 150 Da
  • Affinity range: 1 mM to 10 pM
  • Association rate range: 10¹ to 10⁷ M⁻¹s⁻¹
  • Dissociation rate range: 10⁻⁶ to 0.1 s⁻¹

Sample Constraints

  • Sample input format: 96-well
  • Minimum sample volume: 180 μL
  • Recoverable samples/non-destructive analysis
  • Sample types:
    • Proteins/Peptides
    • Small molecules/Fragments
    • Oligonucleotides/DNA/RNA
    • Virus-like particles
    • Antibodies
    • Serum containing media
    • DMSO containing buffers
    • Crude cell lysates

ExperimentDifferentialScanningCalorimetry[]

Differential Scanning Calorimeter

  • Capillary based liquid sample cells
  • 500 to 700 µL sample and reference cell volume
  • 10°C per hour to 300°C per hour temperature scan rate
  • 1°C minimum temperature to 140°C maximum temperature (± 0.1°C precision)
  • 1.25 µCal/°C energy repeatability

Autosampler

  • Sample incubation at 4 to 40°C
  • 6x SBS plates per batch

ExperimentIsothermalTitrationCalorimetry[]

Isothermal Titration Calorimeter

  • 190 µL active cell volume
  • Up to 250 µL injection volume
  • 2 to 80 °C temperature range
  • 0.05 to 5,000 µJ measurable heat range
  • 0 to 200 RPM stirring
  • Automated injection syringe cleaning
  • Refrigerated autosampler compatible with 96-well plate

ExperimentUVMelting[]

Example applications include: Thermal melting curves, Nucleic acid melting point measurement

UV/Vis Spectrometer with Thermal Controller

  • Simultaneously scan a full wavelength range, on 8 channels, in less than a second
  • Each pair of cuvettes can be held at a different temperature: Perform 4 different temperature experiments at the same time
  • Simultaneously measure standards, samples, and controls under exactly the same conditions
  • Measurement modules do not contain any moving parts (permanent optical alignment)

Absorbance Detection

  • Photometric system: Double beam with rear beam access
  • Long-life xenon flashlamp source
  • 250 points per second data collection rate
  • 190 to 1,100 nm wavelength range (limiting resolution 0.1 nm, wavelength accuracy ± 0.2 nm)
  • 150,000 nm/min maximum scan rate
  • Tightly-controlled beam geometry (< 1.5 mm at sample interface)
  • Silicon photodiode detectors for simultaneous measurement of all channels
  • 8 cuvette positions can be measured simultaneously

Thermodynamics

  • Temperature control system is self-contained, integrated, and air-cooled
  • Water-free temperature cycling from 0 to 110°C
  • Peltier block probe ± 0.5°C temperature accuracy
  • Sample probe ± 0.25ºC temperature accuracy
  • < ± 0.15°C cell to cell variation
  • Maximum ramp rate 40°C/min
  • Minimum ramp rate 0.1°C/min
  • 1, 2, or 4 independently controlled temperature zones

ExperimentThermalShift[]

Example applications include: Multimode Spectrophotometer, Thermal Stability, Conformation Change Detection, Protein Aggregation Detection, Melting Curve Analysis, Aggregation Curve Analysis

qPCR Thermocycler

Excitation Filters

  • 470 nm (15 nm bandwidth)
  • 520 nm (10 nm bandwidth)
  • 550 nm (10 nm bandwidth)
  • 580 nm (10 nm bandwidth)
  • 640 nm (10 nm bandwidth)
  • 662 nm (10 nm bandwidth)

Emission Filters

  • 520 nm (15 nm bandwidth)
  • 558 nm (12 nm bandwidth)
  • 587 nm (10 nm bandwidth)
  • 623 nm (14 nm bandwidth)
  • 682 nm (14 nm bandwidth)
  • 711 nm (12 nm bandwidth)

Halogen Lamp Reaction Conditions

  • 4 to 100°C temperature range
  • 204°C/min maximum temperature ramp rate
  • Programmable thermal cycle program (up to 100 cycles)
  • Reactions in 384-well plates

Multimode Spectrophotometer

  • Simultaneously measure fluorescence spectra and static light scattering of samples enabling assessment of melting point and aggregation point
  • Measurement in sealed quartz capillaries requires only 9 μL of sample
  • Measure up to 48 samples in one experiment including standards, samples, and controls

Fluorescence and Static Light Scattering Detection

  • 266 nm and 473 nm laser light sources with neutral density filtering available
  • CCD spectrometer at full 250 to 750 nm spectral range simultaneously detects fluorescence emission spectra and static light scattering intensity at 266 nm and 473 nm
  • Melting point precision < 2% CV
  • Static light scattering resolution can detect ~15 kDa change in mean molecular mass

Thermodynamics

  • Temperature control system is self-contained
  • Water-free temperature cycling from 15 to 95ºC
  • TEC heating/cooling plate temperature control accuracy 1ºC (at < 70ºC) and 1.5ºC (at > 70ºC)
  • Maximum ramp rate 10ºC/min
  • Minimum ramp rate 0.1ºC/min

ExperimentTotalProteinQuantification[]

Example applications include: Bradford Assay, Bicinchoninic Acid (BCA) Assay, Quant-iT Fluorescence Quantification Assay, Total Protein Quantification, Protein Concentration Quantification

Total Protein Quantification

  • Absorbance- and fluorescence-based assays
  • Customizable excitation and quantification wavelengths
  • Protein quantification between 0.025 and 2 mg/mL
  • Between 5 and 25 µL of sample required
  • Bovine serum albumin or bovine gamma globulin standards

Instrumentation

  • Reagent preparation, plate loading, and mixing performed on Hamilton liquid handler
  • Absorbance measured on FLUOstar Omega plate reader
  • Fluorescence measured on CLARIOstar plate reader

ExperimentAlphaScreen[]

Example applications include: Bead-based Interaction Assay, Protein-protein Interaction Screening, Binding Inhibitor Screening, Protease/kinase Inhibitor Screening, Binding And Dissociation Analysis

Amplified Luminescent Proximity Homogenous Assay Screen (AlphaScreen)

  • Bead-based assay
  • AlphaScreen laser and filter sets
  • Selection of beads with a variety of affinity tags
  • Support 96- or 384-well plate format
  • 10 to 160 µL assay volume

Assays Available

  • Screen Optimization
  • Interaction Characterization
  • Interaction Inhibitor Screen
  • Interaction Inhibitor Dose Response
  • Enzyme Activity Characterization
  • Enzyme Inhibitor Screen
  • Enzyme Inhibitor Dose Response
  • Linker Screen
  • Sample Quantification

ExperimentBioconjugation[]

Example applications include: Chemical crosslinking, Protein modification, Antibody modification, Fluorophore conjugated, Biotinylated

Liquid Handler

  • Low-volume chemical linking of biomolecules including microbeads, oligomers, proteins, and antibodies
  • Utilizes all available solid transfer, liquid transfer, and mixing instrumentation
  • Biomolecule activation can be specified prior to conjugation.
  • Reaction quenching can be specified after conjugation
  • Product workup including pelleting and filtering can be specified after conjugation

ExperimentBioAnalyzer[]

Fragment Analyzer

  • Medium- to high-throughput separation – Analyze 48 or 96 samples in parallel with a single, simple dilution step and easy instrument setup
  • Unattended walk-away operation – Load up to three 96-sample microplates, no further user interaction is required
  • Reduced sample analysis time – Apply batch processing features post electrophoresis to automatically generate results reports
  • Easy adoption to changes in your workflow – Choose a shorter or longer array to maximize your runs regarding speed or resolution of individual samples
  • Minimize hands-on time – Reduce the time needed for instrument setup with no daily array handling requirements and room-temperature-stable reagents
  • Broad assay portfolio – Analyze samples ranging from gDNA, NGS libraries, and DNA fragments to total RNA, small RNA, and mRNA vaccines all on one platform
  • High-resolution separation – Achieve separation resolution as good as 3 bp from fragments under 300 bp
  • Objective quality assessment – Qualify your samples using quality metrics for RNA (RQN) and genomic DNA (GQN)
  • Seamless switching between applications – Load two different gels into the instrument to use different kits within one system setup
  • Comprehensive compliance services – Qualify instruments by IQ/OQ services for use in GMP environments
Agilent – 5300 Fragment Analyzer