SCIENTIFIC INSTRUMENTATION

Single Cell Instrument

• Characterize complex cell populations
• Multiomic profiling of immune diversity, cell by cell

Capillary ELISA Device

• All incubation, washing, and detection steps are automated
• 72 samples with 1 analytes, or 32 samples with up to 8 analytes
• Built-in duplicate or triplicate measurement per sample
• 25 µL volume required per sample
• 4 to 5 logs of dynamic range
• 75 minute run time

Multiplexing ELISA Screening Device

• Magnetic bead-based immunoassays
• Up to 50-analyte multiplexing per sample
• 35 to 60ºC sample temperature control
• 3.5 logs of dynamic range
• 96-well plate assay in less than an hour
• CCD imager detection

Alternative Next Generation Sequencing Machine

• Output Range: 540 Mb – 15 Gb
• Reads per Run: 1-25 million
• Max read length: 2x 300 bp

Next Generation Sequencing Machine

• Compatible with various library preparation kits
• Mix and match between four fow cell types
• 532, 660, 780, and 790 nm lasers
• Highly fexible – outputs between 80 Gb and 800 million reads to 6 Tb and 20 billion reads in less than 2 days

Capillary Electrophoresis System

• Sample Trays: 2 x 48 CESI vials / 0.3 mL microvials
• Buffer Tray: 2 x 36 CESI vials
• Pressure Delivery Range: -5 to +100 PSI
• Required Sample Volume: 5 µL when using NanoVias
• Zero Dead Volume
• Multi-Detection Capability: UV/VIS, PDA, or LIF detection can be selected in stand-alone CE mode
• Ultra-low-fow < 10 nLimin, into the mass spectrometer resulting an overall increase in ionization efficiency

Tape Station

• Sample number: 1 to 96
• Sample volume: 1 to 2 µL
• Sizing range: 200 to > 60,000 bp
• Sensitivity: 0.5 ng/µL
• Sizing precision: 200 bp to 15,000 bp: 15 %CV
• Sizing accuracy: 200 bp to 15,000 bp: ± 15 %
• Quantitative precision 15 %CV
• Quantitative accuracy: ± 20 %
• Quantitative range: 10 to 100 ng/µL
• DNA Integrity Number functional range: 5 to 300 ng/µL

Capillary Western Blot System

Capillary Gel Electrophoresis

• 2 to 440 kDa molecular weight range
• 0 to 500 V separation voltage
• UV capillary crosslinking for protein immobilization

Secondary Antibodies Available

• Secondary Antibodies Available
  • Goat Anti-Rabbit HRP
  • Goat Anti-Mouse HRP
  • Goat Anti-Human IgG HRP
  • Donkey Anti-Goat HRP

CCD Camera-Based Capillary Imaging

• 100 ms to 512 sec exposure times
• Up to picogram sensitivity (depending on the primary antibody sensitivity)

Capacity

• 13 or 25 capillaries per batch
• 5 to 8 µL of sample required per capillary

qPCR Thermocycler

Excitation Filters

• 470 nm (15 nm bandwidth)
• 520 nm (10 nm bandwidth)
• 550 nm (10 nm bandwidth)
• 580 nm (10 nm bandwidth)
• 640 nm (10 nm bandwidth)
• 662 nm (10 nm bandwidth)

Emission Filters

• 520 nm (15 nm bandwidth)
• 558 nm (12 nm bandwidth)
• 587 nm (10 nm bandwidth)
• 623 nm (14 nm bandwidth)
• 682 nm (14 nm bandwidth)
• 711 nm (12 nm bandwidth)

Halogen Lamp Reaction Conditions

• 4 to 100°C temperature range
• 204°C/min maximum temperature ramp rate
• Programmable thermal cycle program (up to 100 cycles)
• Reactions in 384-well plates or array cards

Digital PCR System

• Up to 480 samples per run
• Absolute quantification of DNA/RNA targets
• Dynamic range: 0.25 to 5,000 targets per μL sample
• Multiplexing with 4 detection channels per sample
• Multiplex up to 8 targets per sample with amplitude multiplexing

Sample Constraints

• 20 μL sample volume
• 96-well plate format
• Fluorescent probe-based detection

Droplet Generation

• In-plate microfluidics for droplet formation
• Up to 20,000 nanoliter-sized droplets per sample
• Droplet generation for 16 wells in parallel

Thermocycling

• 100°C maximum temperature
• 150°C/min maximum temperature ramp rate
• Programmable temperature gradient over columns
• Programmable thermal cycling (Up to 99 cycles)

Droplet Reading

• Sample illumination: LED
  • 505 nm (30 nm bandwidth)
  • 622 nm (15 nm bandwidth)
• Detector: Multi-pixel Photon Counter
• Emission filters
  • 538 nm (25 nm bandwidth)
  • 572 nm (40 nm bandwidth)
  • 662 nm (40 nm bandwidth)
  • 720 nm (60 nm bandwidth)

Surface Plasmon Resonance System

• Flow cells: 4
• Flow rate range: 1 to 100 µL/min
• Analyte detection range: No lower molecular weight limit for organic molecules
• Association rate constant range (protein): 103 to 3×109 M-1s-1
• Association rate constant range (low molecular weight): 103 to 5×107 M-1s-1
• Dissociation rate constant range: 10-5 to 1 s-1
• Sample temperature range: 4 to 45°C
• Analysis temperature range: 4 to 45ºC
• Sample refractive index range: 1.33 to 1.40
• Automatic switching between 4 buffers

Data Types

• Kinetic and affinity
• Specificity
• Selectivity
• Concentration
• Thermodynamic

Sample Constraints

• Sample input format: 96- or 384-well and up to 33 vials
• Sample injection volume: 2 to 350 µL
• Injection dead volume: 20 to 50 µL

Bio-Layer Interferometer (BLI)

Instrument Details

• 8 parallel channels per read
• Sample temperature range: 15 to 40°C in 1°C increments
• Orbital flow capacity: Static or 100 to 1,500 RPM
• Uses commercially available bio-probes
• Baseline noise: < 3 pm

Experiment Types

• Kinetics
  • Measure association and dissociation rates
  • Up to 12 samples per plate
• Quantitation
  • Measure analyte concentration
  • Up to 96 samples per plate
• Epitope binning/cross-blocking
  • Determine modes of interaction through analyte competition
  • Up to 8 samples per plate

Detection Range

• Analyte molecular weight: > 150 Da
• Affinity range: 1 mM to 10 pM
• Association rate range: 10¹ to 10⁷ M⁻¹s⁻¹
• Dissociation rate range: 10⁻⁶ to 0.1 s⁻¹

Sample Constraints

• Sample input format: 96-well
• Minimum sample volume: 180 μL
• Recoverable samples/non-destructive analysis
• Sample types:
  • Proteins/Peptides
  • Small molecules/Fragments
  • Oligonucleotides/DNA/RNA
  • Virus-like particles
  • Antibodies
  • Serum containing media
  • DMSO containing buffers
  • Crude cell lysates

Differential Scanning Calorimeter

• Capillary based liquid sample cells
• 500 to 700 µL sample and reference cell volume
• 10°C per hour to 300°C per hour temperature scan rate
• 1°C minimum temperature to 140°C maximum temperature (± 0.1°C precision)
• 1.25 µCal/°C energy repeatability

Autosampler

• Sample incubation at 4 to 40°C
• 6x SBS plates per batch

Isothermal Titration Calorimeter

• 190 µL active cell volume
• Up to 250 µL injection volume
• 2 to 80 °C temperature range
• 0.05 to 5,000 µJ measurable heat range
• 0 to 200 RPM stirring
• Automated injection syringe cleaning
• Refrigerated autosampler compatible with 96-well plate

UV/Vis Spectrometer with Thermal Controller

• Simultaneously scan a full wavelength range, on 8 channels, in less than a second
• Each pair of cuvettes can be held at a different temperature: Perform 4 different temperature experiments at the same time
• Simultaneously measure standards, samples, and controls under exactly the same conditions
• Measurement modules do not contain any moving parts (permanent optical alignment)

Absorbance Detection

• Photometric system: Double beam with rear beam access
• Long-life xenon flashlamp source
• 250 points per second data collection rate
• 190 to 1,100 nm wavelength range (limiting resolution 0.1 nm, wavelength accuracy ± 0.2 nm)
• 150,000 nm/min maximum scan rate
• Tightly-controlled beam geometry (< 1.5 mm at sample interface)
• Silicon photodiode detectors for simultaneous measurement of all channels
• 8 cuvette positions can be measured simultaneously

Thermodynamics

• Temperature control system is self-contained, integrated, and air-cooled
• Water-free temperature cycling from 0 to 110°C
• Peltier block probe ± 0.5°C temperature accuracy
• Sample probe ± 0.25ºC temperature accuracy
• < ± 0.15°C cell to cell variation
• Maximum ramp rate 40°C/min
• Minimum ramp rate 0.1°C/min
• 1, 2, or 4 independently controlled temperature zones

qPCR Thermocycler

Excitation Filters

• 470 nm (15 nm bandwidth)
• 520 nm (10 nm bandwidth)
• 550 nm (10 nm bandwidth)
• 580 nm (10 nm bandwidth)
• 640 nm (10 nm bandwidth)
• 662 nm (10 nm bandwidth)

Emission Filters

• 520 nm (15 nm bandwidth)
• 558 nm (12 nm bandwidth)
• 587 nm (10 nm bandwidth)
• 623 nm (14 nm bandwidth)
• 682 nm (14 nm bandwidth)
• 711 nm (12 nm bandwidth)

Halogen Lamp Reaction Conditions

• 4 to 100°C temperature range
• 204°C/min maximum temperature ramp rate
• Programmable thermal cycle program (up to 100 cycles)
• Reactions in 384-well plates

Multimode Spectrophotometer

• Simultaneously measure fluorescence spectra and static light scattering of samples enabling assessment of melting point and aggregation point
• Measurement in sealed quartz capillaries requires only 9 μL of sample
• Measure up to 48 samples in one experiment including standards, samples, and controls

Fluorescence and Static Light Scattering Detection

• 266 nm and 473 nm laser light sources with neutral density filtering available
• CCD spectrometer at full 250 to 750 nm spectral range simultaneously detects fluorescence emission spectra and static light scattering intensity at 266 nm and 473 nm
• Melting point precision < 2% CV
• Static light scattering resolution can detect ~15 kDa change in mean molecular mass

Thermodynamics

• Temperature control system is self-contained
• Water-free temperature cycling from 15 to 95ºC
• TEC heating/cooling plate temperature control accuracy 1ºC (at < 70ºC) and 1.5ºC (at > 70ºC)
• Maximum ramp rate 10ºC/min
• Minimum ramp rate 0.1ºC/min

Total Protein Quantification

• Absorbance- and fluorescence-based assays
• Customizable excitation and quantification wavelengths
• Protein quantification between 0.025 and 2 mg/mL
• Between 5 and 25 µL of sample required
• Bovine serum albumin or bovine gamma globulin standards

Instrumentation

• Reagent preparation, plate loading, and mixing performed on Hamilton liquid handler
• Absorbance measured on FLUOstar Omega plate reader
• Fluorescence measured on CLARIOstar plate reader

Amplified Luminescent Proximity Homogenous Assay Screen (AlphaScreen)

• Bead-based assay
• AlphaScreen laser and filter sets
• Selection of beads with a variety of affinity tags
• Support 96- or 384-well plate format
• 10 to 160 µL assay volume

Assays Available

• Screen Optimization
• Interaction Characterization
• Interaction Inhibitor Screen
• Interaction Inhibitor Dose Response
• Enzyme Activity Characterization
• Enzyme Inhibitor Screen
• Enzyme Inhibitor Dose Response
• Linker Screen
• Sample Quantification

Liquid Handler

• Low-volume chemical linking of biomolecules including microbeads, oligomers, proteins, and antibodies
• Utilizes all available solid transfer, liquid transfer, and mixing instrumentation
• Biomolecule activation can be specified prior to conjugation.
• Reaction quenching can be specified after conjugation
• Product workup including pelleting and filtering can be specified after conjugation

Fragment Analyzer

• Medium- to high-throughput separation – Analyze 48 or 96 samples in parallel with a single, simple dilution step and easy instrument setup
• Unattended walk-away operation – Load up to three 96-sample microplates, no further user interaction is required
• Reduced sample analysis time – Apply batch processing features post electrophoresis to automatically generate results reports
• Easy adoption to changes in your workflow – Choose a shorter or longer array to maximize your runs regarding speed or resolution of individual samples
• Minimize hands-on time – Reduce the time needed for instrument setup with no daily array handling requirements and room-temperature-stable reagents
• Broad assay portfolio – Analyze samples ranging from gDNA, NGS libraries, and DNA fragments to total RNA, small RNA, and mRNA vaccines all on one platform
• High-resolution separation – Achieve separation resolution as good as 3 bp from fragments under 300 bp
• Objective quality assessment – Qualify your samples using quality metrics for RNA (RQN) and genomic DNA (GQN)
• Seamless switching between applications – Load two different gels into the instrument to use different kits within one system setup
• Comprehensive compliance services – Qualify instruments by IQ/OQ services for use in GMP environments