SCIENTIFIC INSTRUMENTATION

Single Cell Instrument

 

• Characterize complex cell populations

• Multiomic profiling of immune diversity, cell by cell

 

Capillary ELISA Device

 

• All incubation, washing, and detection steps are automated

• 72 samples with 1 analytes, or 32 samples with up to 8 analytes

• Built-in duplicate or triplicate measurement per sample

• 25 µL volume required per sample

• 4 to 5 logs of dynamic range

• 75 minute run time

Multiplexing ELISA Screening Device

 

• Magnetic bead-based immunoassays

• Up to 50-analyte multiplexing per sample

• 35 to 60ºC sample temperature control

• 3.5 logs of dynamic range

• 96-well plate assay in less than an hour

• CCD imager detection

Alternative Next Generation Sequencing Machine

 

• Output Range: 540 Mb – 15 Gb

• Reads per Run: 1-25 million

• Max read length: 2x 300 bp

 

Next Generation Sequencing Machine

 

• Compatible with various library preparation kits

• Mix and match between four fow cell types

• 532, 660, 780, and 790 nm lasers

• Highly fexible – outputs between 80 Gb and 800 million reads to 6 Tb and 20 billion reads in less than 2 days

Capillary Electrophoresis System

 

• Sample Trays: 2 x 48 CESI vials / 0.3 mL microvials

• Buffer Tray: 2 x 36 CESI vials

• Pressure Delivery Range: -5 to +100 PSI

• Required Sample Volume: 5 µL when using NanoVias

• Zero Dead Volume

• Multi-Detection Capability: UV/VIS, PDA, or LIF detection can be selected in stand-alone CE mode

• Ultra-low-fow < 10 nLimin, into the mass spectrometer resulting an overall increase in ionization efficiency

Tape Station

 

• Sample number: 1 to 96

• Sample volume: 1 to 2 µL

• Sizing range: 200 to > 60,000 bp

• Sensitivity: 0.5 ng/µL

• Sizing precision: 200 bp to 15,000 bp: 15 %CV

• Sizing accuracy: 200 bp to 15,000 bp: ± 15 %

• Quantitative precision 15 %CV

• Quantitative accuracy: ± 20 %

• Quantitative range: 10 to 100 ng/µL

• DNA Integrity Number functional range: 5 to 300 ng/µL

Capillary Western Blot System

Capillary Gel Electrophoresis

 

• 2 to 440 kDa molecular weight range

• 0 to 500 V separation voltage

• UV capillary crosslinking for protein immobilization

Secondary Antibodies Available

 

• Secondary Antibodies Available

   

  • Goat Anti-Rabbit HRP
  • Goat Anti-Mouse HRP
  • Goat Anti-Human IgG HRP
  • Donkey Anti-Goat HRP

CCD Camera-Based Capillary Imaging

 

• 100 ms to 512 sec exposure times

• Up to picogram sensitivity (depending on the primary antibody sensitivity)

Capacity

 

• 13 or 25 capillaries per batch

• 5 to 8 µL of sample required per capillary

qPCR Thermocycler

Excitation Filters

 

• 470 nm (15 nm bandwidth)

• 520 nm (10 nm bandwidth)

• 550 nm (10 nm bandwidth)

• 580 nm (10 nm bandwidth)

• 640 nm (10 nm bandwidth)

• 662 nm (10 nm bandwidth)

Emission Filters

 

• 520 nm (15 nm bandwidth)

• 558 nm (12 nm bandwidth)

• 587 nm (10 nm bandwidth)

• 623 nm (14 nm bandwidth)

• 682 nm (14 nm bandwidth)

• 711 nm (12 nm bandwidth)

Halogen Lamp Reaction Conditions

 

• 4 to 100°C temperature range

• 204°C/min maximum temperature ramp rate

• Programmable thermal cycle program (up to 100 cycles)

• Reactions in 384-well plates or array cards

 

Digital PCR System

 

• Up to 480 samples per run

• Absolute quantification of DNA/RNA targets

• Dynamic range: 0.25 to 5,000 targets per μL sample

• Multiplexing with 4 detection channels per sample

• Multiplex up to 8 targets per sample with amplitude multiplexing

Sample Constraints

 

• 20 μL sample volume

• 96-well plate format

• Fluorescent probe-based detection

Droplet Generation

 

• In-plate microfluidics for droplet formation

• Up to 20,000 nanoliter-sized droplets per sample

• Droplet generation for 16 wells in parallel

Thermocycling

 

• 100°C maximum temperature

• 150°C/min maximum temperature ramp rate

• Programmable temperature gradient over columns

• Programmable thermal cycling (Up to 99 cycles)

Droplet Reading

 

• Sample illumination: LED

   

  • 505 nm (30 nm bandwidth)
  • 622 nm (15 nm bandwidth)

• Detector: Multi-pixel Photon Counter

• Emission filters

   

  • 538 nm (25 nm bandwidth)
  • 572 nm (40 nm bandwidth)
  • 662 nm (40 nm bandwidth)
  • 720 nm (60 nm bandwidth)

 

Surface Plasmon Resonance System

 

• Flow cells: 4

• Flow rate range: 1 to 100 µL/min

• Analyte detection range: No lower molecular weight limit for organic molecules

• Association rate constant range (protein): 103 to 3×109 M-1s-1

• Association rate constant range (low molecular weight): 103 to 5×107 M-1s-1

• Dissociation rate constant range: 10-5 to 1 s-1

• Sample temperature range: 4 to 45°C

• Analysis temperature range: 4 to 45ºC

• Sample refractive index range: 1.33 to 1.40

• Automatic switching between 4 buffers

Data Types

 

• Kinetic and affinity

• Specificity

• Selectivity

• Concentration

• Thermodynamic

Sample Constraints

 

• Sample input format: 96- or 384-well and up to 33 vials

• Sample injection volume: 2 to 350 µL

• Injection dead volume: 20 to 50 µL

Bio-Layer Interferometer (BLI)

Instrument Details

 

• 8 parallel channels per read

• Sample temperature range: 15 to 40°C in 1°C increments

• Orbital flow capacity: Static or 100 to 1,500 RPM

• Uses commercially available bio-probes

• Baseline noise: < 3 pm

Experiment Types

 

• Kinetics

   

  • Measure association and dissociation rates
  • Up to 12 samples per plate

• Quantitation

   

  • Measure analyte concentration
  • Up to 96 samples per plate

• Epitope binning/cross-blocking

   

  • Determine modes of interaction through analyte competition
  • Up to 8 samples per plate

Detection Range

 

• Analyte molecular weight: > 150 Da

• Affinity range: 1 mM to 10 pM

• Association rate range: 10¹ to 10⁷ M⁻¹s⁻¹

• Dissociation rate range: 10⁻⁶ to 0.1 s⁻¹

Sample Constraints

 

• Sample input format: 96-well

• Minimum sample volume: 180 μL

• Recoverable samples/non-destructive analysis

• Sample types:

   

  • Proteins/Peptides
  • Small molecules/Fragments
  • Oligonucleotides/DNA/RNA
  • Virus-like particles
  • Antibodies
  • Serum containing media
  • DMSO containing buffers
  • Crude cell lysates

Differential Scanning Calorimeter

 

• Capillary based liquid sample cells

• 500 to 700 µL sample and reference cell volume

• 10°C per hour to 300°C per hour temperature scan rate

• 1°C minimum temperature to 140°C maximum temperature (± 0.1°C precision)

• 1.25 µCal/°C energy repeatability

Autosampler

 

• Sample incubation at 4 to 40°C

• 6x SBS plates per batch

Isothermal Titration Calorimeter

 

• 190 µL active cell volume

• Up to 250 µL injection volume

• 2 to 80 °C temperature range

• 0.05 to 5,000 µJ measurable heat range

• 0 to 200 RPM stirring

• Automated injection syringe cleaning

• Refrigerated autosampler compatible with 96-well plate

UV/Vis Spectrometer with Thermal Controller

 

• Simultaneously scan a full wavelength range, on 8 channels, in less than a second

• Each pair of cuvettes can be held at a different temperature: Perform 4 different temperature experiments at the same time

• Simultaneously measure standards, samples, and controls under exactly the same conditions

• Measurement modules do not contain any moving parts (permanent optical alignment)

Absorbance Detection

 

• Photometric system: Double beam with rear beam access

• Long-life xenon flashlamp source

• 250 points per second data collection rate

• 190 to 1,100 nm wavelength range (limiting resolution 0.1 nm, wavelength accuracy ± 0.2 nm)

• 150,000 nm/min maximum scan rate

• Tightly-controlled beam geometry (< 1.5 mm at sample interface)

• Silicon photodiode detectors for simultaneous measurement of all channels

• 8 cuvette positions can be measured simultaneously

Thermodynamics

 

• Temperature control system is self-contained, integrated, and air-cooled

• Water-free temperature cycling from 0 to 110°C

• Peltier block probe ± 0.5°C temperature accuracy

• Sample probe ± 0.25ºC temperature accuracy

• < ± 0.15°C cell to cell variation

• Maximum ramp rate 40°C/min

• Minimum ramp rate 0.1°C/min

• 1, 2, or 4 independently controlled temperature zones

qPCR Thermocycler

Excitation Filters

 

• 470 nm (15 nm bandwidth)

• 520 nm (10 nm bandwidth)

• 550 nm (10 nm bandwidth)

• 580 nm (10 nm bandwidth)

• 640 nm (10 nm bandwidth)

• 662 nm (10 nm bandwidth)

Emission Filters

 

• 520 nm (15 nm bandwidth)

• 558 nm (12 nm bandwidth)

• 587 nm (10 nm bandwidth)

• 623 nm (14 nm bandwidth)

• 682 nm (14 nm bandwidth)

• 711 nm (12 nm bandwidth)

Halogen Lamp Reaction Conditions

 

• 4 to 100°C temperature range

• 204°C/min maximum temperature ramp rate

• Programmable thermal cycle program (up to 100 cycles)

• Reactions in 384-well plates

Multimode Spectrophotometer

 

• Simultaneously measure fluorescence spectra and static light scattering of samples enabling assessment of melting point and aggregation point

• Measurement in sealed quartz capillaries requires only 9 μL of sample

• Measure up to 48 samples in one experiment including standards, samples, and controls

Fluorescence and Static Light Scattering Detection

 

• 266 nm and 473 nm laser light sources with neutral density filtering available

• CCD spectrometer at full 250 to 750 nm spectral range simultaneously detects fluorescence emission spectra and static light scattering intensity at 266 nm and 473 nm

• Melting point precision < 2% CV

• Static light scattering resolution can detect ~15 kDa change in mean molecular mass

Thermodynamics

 

• Temperature control system is self-contained

• Water-free temperature cycling from 15 to 95ºC

• TEC heating/cooling plate temperature control accuracy 1ºC (at < 70ºC) and 1.5ºC (at > 70ºC)

• Maximum ramp rate 10ºC/min

• Minimum ramp rate 0.1ºC/min

Total Protein Quantification

 

• Absorbance- and fluorescence-based assays

• Customizable excitation and quantification wavelengths

• Protein quantification between 0.025 and 2 mg/mL

• Between 5 and 25 µL of sample required

• Bovine serum albumin or bovine gamma globulin standards

Instrumentation

 

• Reagent preparation, plate loading, and mixing performed on Hamilton liquid handler

• Absorbance measured on FLUOstar Omega plate reader

• Fluorescence measured on CLARIOstar plate reader

Amplified Luminescent Proximity Homogenous Assay Screen (AlphaScreen)

 

• Bead-based assay

• AlphaScreen laser and filter sets

• Selection of beads with a variety of affinity tags

• Support 96- or 384-well plate format

• 10 to 160 µL assay volume

Assays Available

 

• Screen Optimization

• Interaction Characterization

• Interaction Inhibitor Screen

• Interaction Inhibitor Dose Response

• Enzyme Activity Characterization

• Enzyme Inhibitor Screen

• Enzyme Inhibitor Dose Response

• Linker Screen

• Sample Quantification

Liquid Handler

 

• Low-volume chemical linking of biomolecules including microbeads, oligomers, proteins, and antibodies

• Utilizes all available solid transfer, liquid transfer, and mixing instrumentation

• Biomolecule activation can be specified prior to conjugation.

• Reaction quenching can be specified after conjugation

• Product workup including pelleting and filtering can be specified after conjugation

Fragment Analyzer

 

• Medium- to high-throughput separation – Analyze 48 or 96 samples in parallel with a single, simple dilution step and easy instrument setup

• Unattended walk-away operation – Load up to three 96-sample microplates, no further user interaction is required

• Reduced sample analysis time – Apply batch processing features post electrophoresis to automatically generate results reports

• Easy adoption to changes in your workflow – Choose a shorter or longer array to maximize your runs regarding speed or resolution of individual samples

• Minimize hands-on time – Reduce the time needed for instrument setup with no daily array handling requirements and room-temperature-stable reagents

• Broad assay portfolio – Analyze samples ranging from gDNA, NGS libraries, and DNA fragments to total RNA, small RNA, and mRNA vaccines all on one platform

• High-resolution separation – Achieve separation resolution as good as 3 bp from fragments under 300 bp

• Objective quality assessment – Qualify your samples using quality metrics for RNA (RQN) and genomic DNA (GQN)

• Seamless switching between applications – Load two different gels into the instrument to use different kits within one system setup

• Comprehensive compliance services – Qualify instruments by IQ/OQ services for use in GMP environments