SCIENTIFIC INSTRUMENTATION


SAMPLE PREPARATION

ExperimentMix[]

ExperimentIncubate[]

Example applications include: Mix until Dissolved (Homogeneous), Mix while Heating/Cooling, Tissue Homogenization/Sonication

Overhead Mixer

    • Stainless IKA steel stirrers
    • 50 to 1,000 RPM stir rate
    • Room temperature (22°C ± 0.45°C (1 sigma)) to 500ºC temperature control
    • Available for 100 mL glass bottles to 20 L carboys volume ranges

Vortex

Tube
    • 600 to 3,200 RPM
    • Available for 1 to 50 mL volume ranges
    • Holds 6x 50 mL tube, 12x microcentrifuge tube, 6x 15 mL tube
    • Holds 4x 50 mL tubes, 4x 15 mL tubes
    • Holds 4x 100 mL glass bottles
Microplate
    • 100 to 3,200 RPM
    • Holds 4x microplates
    • Room temperature (22ºC) to 70ºC in 0.1ºC increments
    • Heated lid to prevent condensation
Tube & Microplate
    • -20 to 100ºC in 1ºC increments
    • 200 to 1000 RPM in 100 RPM increments
    • Holds 1x microplate
    • Holds 24x 2 mL tubes
    • Holds 6x 50 mL tubes

Rollers

Tube Roller
    • 1 to 80 RPM roller rate
    • 22ºC (room temperature) to 75ºC
    • Available for
        • 2 mL, 15 mL, and 50 mL tubes
        • 10 L and 20 L carboys
        • 1 L, 2 L, and 5 L bottles
Drum Roller
    • 40 RPM stir rate
    • Available for 5 to 10 L carboys
Medium Bottle Roller
    • 5 to 80 RPM stir rate
    • Available for 100 mL to 2 L bottles

Hot Plate

    • 22ºC (room temperature) to 500ºC
    • Available for 100 mL glass bottle to 20 L carboy volume ranges

Heat Bath

Heating
    • 22ºC (room temperature) to 150°C
    • Container capacity per batch
        • 150x microcentrifuge tubes
        • 2x SBS plates
        • 12x 15 mL tubes
        • 5x 50 mL tubes
        • 2x 250 mL bottles
        • 2x 500 mL bottles
Cooling
    • 22ºC (room temperature) to -20°C
    • Container capacity per batch
        • 340x microcentrifuge tubes
        • 2x SBS plates
        • 30x 15 mL tubes
        • 16x 50 mL tubes
        • 4x 250 mL bottles
        • 2x 500 mL bottles

Heater Shaker

    • 35 to 1,000 RPM shake rate
    • Available for 1 mL to 1 L volume ranges
    • Room temperature (25°C ± 0.45°C (1 sigma)) to 75ºC temperature control

Water Bath

    • Room temperature to 100ºC in 0.1ºC increments
    • Temperature accuracy of 0.24ºC
    • Available for 2 mL tubes to 250 mL glass bottles

Bath Sonicator

    • 40 kHz frequency
    • 22 to 69ºC heated water bath
    • Delivers unwavering cavitation
    • Available for 2 mL to 5 L volume ranges
    • High-temp safety alarm indicator
    • High/low power control for normal to delicate applications
    • Tabletop cleaner

Homogenizer

    • 550 W at 20 kHz
    • 22 to 100ºC temperature control
    • Available for 0.2 to 1,000 mL volume ranges
    • Specialized accessories/tools to meet application requirements. 3 different horn/tip types allow us to process samples from 0.2 to 1,000 mL

Plate Sonicator

    • Up to 700 watts power
    • Can mix and perform cell lysis
    • Holds 1x microplate
    • Temperature range 5 to 45°C with recirculating chiller
    • Pulse widths adjustable from 1 second to 24 hours

Cell Disruptor

    • Available for 1.5 mL and 2 mL tubes
    • 1,000 to 3,000 RPM
    • 0.1 mm or 0.5 mm disruptor beads
    • 1 to 75ºC

Automated Thermocycler

    • Temperature accuracy: ± 0.25°C (between 35.0 and 99.9°C)
    • Max block ramp rate: 3.5°C/sec
    • Max sample ramp rate: 1.8°C/sec
    • Temperature range: 4 to 105°C
    • Temperature uniformity: ± 0.50°C (20 sec after reaching 95°C)
Sample Constraints
    • Plate format: 96-well PCR plate
    • Sample volume: 10 to 100 µL

Wrist Action Shaker

    • 10 lb max load
    • 385 oscillations per minute
    • Up to 8x 250 mL flasks or smaller vessels

Nutator

    • 2 to 60 RPM
    • Available for 2 mL, 15 mL, 50 mL tubes, microplates, culture flasks, and dishes
    • 5 to 40ºC

ExperimentCentrifuge[]

Example applications include: Particle separation, Filtration, Pelleting, Density gradient centrifugation

Microcentrifuge

    • 10 to 14,800 RPM (16,166 g at Rmax)
    • 36x tubes per batch
    • Temperature range: -10 to 40ºC

Centrifuge

    • 15 mL conical tubes
        • Up to 4,811 x g
        • Up to 56 tubes per batch
    • 50 mL conical tubes
        • Up to 4,811 x g
        • Up to 16 tubes per batch
    • SBS format plates
        • Up to 3,877 x g
        • Up to 24 regular plates per batch
        • Up to 8 deepwell plates per batch
        • Up to 116 mm plate loading height
        • Temperature range: -10 to 40ºC
    • Array cards
        • Up to 193 x g
        • Up to 12 array cards per batch
        • Temperature range: -10 to 40°C

Ultracentrifuge

    • Max rotation rate: 80,000 RPM
    • Max force: 802,000 x g
    • Speed control: ± 2 RPM of set speed (above 1,000 RPM)
    • Max time: 999 hr 59 min
    • Temperature control
        • Solid state refrigeration
        • Temperature range: 0 to 40°C in 1°C increment
    • Rotors
        • Fixed-Angle Rotors
            • Type 45 Ti Titanium Rotor
                • 94 mL UltraClear tubes
                • Up to 45,000RPM
                • Up to 6 tubes per batch
            • Type 70 Ti Titanium Rotor
                • 32.4 mL OptiSeal tubes
                • Up to 70,000RPM
                • Up to 8 tubes per batch
            • Type 70.1 Ti Titanium Rotor
                • 8.9 mL OptiSeal tubes
                • Up to 70,000RPM
                • Up to 12 tubes per batch
        • Swing-Bucket Rotor
            • SW 32 Ti Rotor
                • 32.4 mL OptiSeal tubes
                • Up to 32,000RPM
                • Up to 6 tubes per batch

ExperimentPellet[]

Microcentrifuge

    • 10 to 14,800 RPM (16,166 g at Rmax)
    • 36x tubes per batch
    • Temperature range: -10 to 40ºC

Centrifuge

    • 15 mL conical tubes
        • Up to 4,811 x g
        • Up to 56 tubes per batch
    • 50 mL conical tubes
        • Up to 4,811 x g
        • Up to 16 tubes per batch
    • SBS format plates
        • Up to 3,877 x g
        • Up to 24 regular plates per batch
        • Up to 8 deepwell plates per batch
        • Up to 116 mm plate loading height
        • Temperature range: -10 to 40ºC

Ultracentrifuge

    • Max rotation rate: 80,000 RPM
    • Max force: 802,000 x g
    • Speed control: ± 2 RPM of set speed (above 1,000 RPM)
    • Max time: 999 hr 59 min
    • Temperature control
        • Solid state refrigeration
        • Temperature range: 0 to 40°C in 1°C increment
    • Rotors
        • Fixed-Angle Rotors
            • Type 45 Ti Titanium Rotor
                • 94 mL UltraClear tubes
                • Up to 45,000RPM
                • Up to 6 tubes per batch
            • Type 70 Ti Titanium Rotor
                • 32.4 mL OptiSeal tubes
                • Up to 70,000RPM
                • Up to 8 tubes per batch
            • Type 70.1 Ti Titanium Rotor
                • 8.9 mL OptiSeal tubes
                • Up to 70,000RPM
                • Up to 12 tubes per batch
        • Swing-Bucket Rotor
            • SW 32 Ti Rotor
                • 32.4 mL OptiSeal tubes
                • Up to 32,000RPM
                • Up to 6 tubes per batch

ExperimentFilter[]

Example applications include: Remove particulate matter, Separate and collect filtrate and retentate separately, Sterilize solutions, Centrifuge filtration, Syringe filtration, Peristaltic pump filtration, Positive air pressure filtration, Vacuum filtration, Büchner funnel filtration

Filter Membrane Housing with Peristaltic Pump

    • 100 mL/min to 2.5 L/min peristaltic pump
    • 4 to 20 L sample volume required

Positive Pressure Filter

    • 0 to 40 PSI independent pressure sources for each well
    • Filter though SBS filter plates
    • Collects in SBS deep well plates

Filter Block with Vacuum Pump

    • 26.4 inHg vacuum pressure
    • 0.5 to 2 mL sample volume required

Filter Tops and Assemblies with Vacuum Pump

    • 26.4 inHg vacuum pressure
    • 50 mL to 1 L sample volume required

Centrifugal Filtration

    • Concentrate proteins, DNA, or other macromolecules
    • Molecular weight cut-off (MWCO): 3,000 to 100,000 Da
    • 0.25 to 0.45 µm pore size
    • 0.5 to 15 mL sample volume required

Syringe Filters

    • 28 mL/min max flow rate
    • 0.1 to 60 mL sample volume required

ExperimentEvaporate[]

Example applications include: Concentrate samples, Dry samples, Remove high boiling point solvents, Reduce bumping on rotary evaporator (rotovap)

Rotovap

    • ~1,000 mBar to ~10 mBar vacuum pressures
    • 6.97 L water bath for 20 to 100°C incubation
    • 300 RPM rotation
    • 50 mL to 3 L round bottom flasks available for evaporation
    • 2 L trap capacity
    • -20°C recirculating condenser, with -105°C supplemental cold finger

Speedvac

Vacuum Centrifuge
    • 5.1 Torr minimum pressure
    • 5 available vacuum ramp rates between 5 Torr/min and 70 Torr/min
    • Vacuum pump displacement of 36 L/min
    • -50°C integrated cold trap
    • Ambient or 35 to 80°C incubation in 5°C increments
    • Sample constraints: 8x SBS style plates per batch

Needle Dryer

    • 1x SBS style deep well plate per batch
    • 96 stainless steel needles
    • Adjustable needle height for precise gas flow delivery
    • 25 to 100°C incubation range
    • 25 L/min maximum nitrogen flow rate

Nitrogen Evaporator

    • 48 nitrogen delivery nozzles
    • 3.5 L/min maximum flow rate per nozzle
    • 25 to 90°C bath temperature control range
    • ± 2°C temperature accuracy
    • Sample constraints:
        • 48x 2 mL tubes per batch
        • 48x 15 mL tubes or test tubes per batch
        • 24x 50 mL tubes per batch

ExperimentLyophilize[]

Lyophilizer

    • < 150 mTorr minimum pressure
    • -55 to 60°C shelf temperature control range
    • -85°C lowest condenser temperature
    • Temperature uniformity of ± 1°C
    • 4 L ice condensing capacity in 24 hours
    • Sample constraints
        • 384x microcentrifuge tubes per batch
        • 8x SBS style plates per batch

ExperimentAutoclave[]

Example applications include: Sterilize instruments, media, and equipment, Steam sterilization, Sterilize biohazardous waste, Selectable autoclave program

Autoclave

    • Chamber volume: 254 L
    • Maximum temperature: 134°C
    • Maximum pressure: 45 PSI
    • Liquid and dry cycles available
    • Programmable cycles (temperature, time)

ExperimentSealPlate[]

PCR Plate Sealer

    • Compatible with unskirted, semi-skirted and skirted PCR plates
    • Plate support blocks available for 96-well and 384-well PCR plates and droplet digital PCR cartridges
    • 100 to 190ºC
    • 0.5 to 10 sec
    • Plate seals
        • Optically clear and peelable heat seal (ideal for PCR/ qPCR)
        • Pierceable foil heat seal (ideal for PCR/ddPCR)
        • Permanent clear heat seal (ideal for long-time water bath cycling)
        • Peelable foil heat seal (ideal for low temperature sample storage)

ExperimentMicrowaveDigestion[]

Example applications include: Digest samples with caustic or oxidizing digestion agents, Prepare samples for elemental composition analysis via Ion Coupled Plasma Mass Spectrometry (ICP-MS), Atomic Emission Spectroscopy (AES), or Atomic Absorption Spectroscopy (AAS), Pressurized microwave reactions, Heat samples with microwave irradiation, Decompose samples, Solubilize heavy metals

Automated Microwave Digestion System

    • Fully digest organic and inorganic samples for elemental analysis
    • Up to 20 mL of prepared sample in digestion mixture in 80 mL reaction vessels
    • Heat samples up to 300 Celsius and pressurize up to 500 PSI, with specified venting parameters
    • Autosampler can load up to 24 samples in one run

ExperimentDegas[]

Example applications include: Organic Synthesis Reactions, Organometallic Reactions, IR Spectroscopy, HPLC, Ion Chromatography, Mass Spectrometry, Cyclic Voltammetry

Freeze-Pump-Thaw Apparatus

    • Flash freezing in liquid nitrogen
    • Vacuum pump pressure: 1×10-4 Torr
    • Water bath for thawing: 22ºC (room temperature) to 180ºC
    • Headspace gas replacement using nitrogen, argon, or helium
    • Available for up to 50 mL volume ranges

Sparging Apparatus

    • Gas options
        • Nitrogen
        • Argon
        • Helium
    • Glass gas dispersion tube
    • Impeller stir bar
    • Gas flow rate: between 20 to 100 mL/min
    • Available for 50 mL to 4 L volume ranges

Vacuum Sonicate Apparatus

    • Vacuum pump pressure: controllable between 1.5 to 770 Torr
    • Sonicator: 40 KHz frequency, 2.5 gallon capacity
    • Headspace gas replacement using nitrogen, argon, or helium
    • Available for 10 mL to 3 L volume ranges

ExperimentFlashFreeze[]

Liquid Nitrogen Submersion

    • Flash freezing samples in liquid nitrogen to -196ºC
    • Available for up to 50 mL volumes
    • Automated liquid nitrogen dispenser for easy dewar filling

ExperimentAdjustpH[]

Immersion pH Meters

    • pH range 0 to 14 pH (± 0.02)
    • 50 µL minimum sample size (consumes no volume)
    • Measures and records pH over time
    • Temperature correction

ExperimentStockSolution[]

Solution Preparation

    • Transfer solids and liquids together
    • Mix and/or heat solution
    • Adjust pH of solution
    • Filter solution
    • Autoclave solution

Volumetric Flasks

    • Provides accurate volume measurements during liquid/slurry transfers
    • Composed of borosilicate 3.3 expansion glass
    • Volumetric Flasks
        • 25 mL
        • 100 mL
        • 250 mL
        • 500 mL
        • 1 L
        • 2 L glass

ExperimentPackColumn[]

Solution Preparation

    • 50 mL to 1 L bed volume for custom columns
    • 0.001 to 50 mLimin fow rate for Avant 25
    • 0.001 to 300 mLimin fow rate for Avant 150
    • 20 MPa maximum pressure for Avant 25
    • 5 MPa maximum pressure for Avant 150
    • 1.4 L mixer volume
    • ± 0.6% gradient composition accuracy for Avant 25
    • ± 0.8% gradient composition accuracy for Avant 150