SCIENTIFIC INSTRUMENTATION

500 MHz NMR

11.74 T Magnet

• 500 MHz for ¹H
• 126 MHz for ¹³C
• 202 MHz for ³¹P
• 470 MHz for ¹⁹F

Probe

• SmartProbe ¹H/¹⁹F/¹³C/³¹P 5 mm probe (-150°C to 150°C)
• Inverse Triple Resonance (TXI) ¹H/¹³C/¹⁹N 5 mm probe (-150°C to 150°C)

Autosampler

• 6 to 51°C incubation temperature
• 480 NMR tubes per batch
• Standard NMR tubes hold 600 µL to 1.5 mL
• Micro NMR tubes hold 40 µL

2D Experiment Types

• Correlation spectroscopy (COSY)
• Double-quantum filtered COSY
• COSY featuring a 45 degree pulse
• Total correlation spectroscopy (TOCSY)
• Heteronuclear single quantum correlation spectroscopy (HSQC, 1H-13C or 1H-15N)
• Heteronuclear multiple quantum correlation spectroscopy (HMQC)
• Heteronuclear multiple-bond correlation spectroscopy (HMBC, 1H-13C or 1H-15N)
• HSQC-TOCSY (1H-13C)
• HMQC-TOCSY (1H-13C)
• Nuclear Overhauser effect spectroscopy (NOESY)
• Rotating-frame Overhauser effect spectroscopy (ROESY)

Microfluidic Spectrometer

Light Source

• Xenon flash lamp

Absorbance Detection

• 250 to 750 nm wavelength selection

Microfluidics

• Fixed path length (0.1 or 0.7 mm) allows for quantification
• Extremely low volumes required (2 μL)
• 96 samples per batch

Multimode Plate Reader w/ Monochromators

Excitation

• Xenon flash lamp
• 1 to 100 flashes per well
• 340 to 740 nm excitation monochromator

Emission

• High voltage PMT detector
• 340 to 740 nm emission monochromator
• Top or bottom of plate read mode

Sample Chamber

• Room temperature (25°C ± 0.45°C (1 sigma)) to 45°C incubation
• Mix by shaking before read options
• 1x microtiter plate (6 to 1,536 well) per batch

Fluorescence Polarization Plate Reader

• Xenon flash lamp
• 1 to 100 flashes per well
• Dual high voltage PMT detectors
• 96-well and 384-well plate compatible
• Excitation Emission Filter Pairs
  • 485 nm, 520 nm
  • 540 nm, 590 nm
  • 625 nm, 675 nm
• Polarized Dual Simultaneous Emission Filters

FTIR Spectrometer

• Deuterated TriGlycine Sulphate (DTGS) Detector
• 375 cm⁻¹ to 7,500 cm⁻¹ spectral range
• > 1,000:1 signal-to-noise ratio
• > 4 cm⁻¹ spectral resolution
• Sample amounts from 20 to 70 µL or 20 to 70 mg required

Multimode Spectrophotometer

Multimode Spectrophotometer

• Measurement in sealed quartz capillaries requires only 9 μL of sample
• Measure up to 48 samples in one experiment
• Z-average diameter, polydispersity, isothermal stability, B22, kD, and G22

Dynamic Light Scattering

• 660 nm laser
• Avalanche photodiode module scattered light detector
• 0.3 to 1,000 nm hydrodynamic radius range
• < 0.1 polydispersity
• 0.05 mg/mL minimum sample concentration
• 192 Da to 25 MDa molecular weight range

Thermodynamics

• Self-contained water-free temperature control system from 15 to 95ºC
• TEC heating/cooling plate temperature control accuracy 1ºC (at < 70ºC) and 1.5ºC (at > 70ºC)

DLS Plate Reader

• 830 nm laser
• 0.5 to 1000 nm hydrodynamic radius range
• 1000 to 1 MDa molecular weight range
• 0.125 mg/mL minimum sample concentration
• 4 to 85ºC temperature control
• Support standard 96, 384 or 1,536 microwell plates
• 4 µL minimum sample volume

All Readers

Sample Chamber

• Room temperature (25°C ± 0.45°C (1 sigma)) to 45°C incubation
• Mix by shaking before read options
• 1x microtiter plate (6 to 1,536 well) per batch

Sample Injection

• 2x 500 µL injection syringes per batch
• 50 µL dead volumes, 0.5 µL precision
• 25 mL/min maximum injection speed
• 2 mL microcentrifuge tubes, 15 mL conical tubes, or 50 mL conical tube sources

Multimode Plate Reader w/ Monochromators

• 96-well plate compatible

Excitation

• Xenon flash lamp
• 1 to 100 flashes per well
• 340 to 740 nm excitation monochromator
• 680 nm laser (AlphaScreen)

Emission

• High voltage PMT detector
• 340 to 740 nm emission monochromator
• Top or bottom of plate read mode

Multimode Plate Reader w/ Filters

• 96-well plate compatible

Excitation

• Xenon flash lamp
• 1 to 100 flashes per well
• 355 nm, 410 nm, 485 nm, 544 nm, 584 nm, 650 nm, or 700 nm fixed wavelength excitation filters (5 nm bandpass)

Emission

• High voltage PMT detector
• 420 nm, 460 nm, 520 nm, 590 nm, 620 nm, or 750 nm fixed wavelength emission filters (5 nm bandpass)
• Top or bottom of plate read mode

Fluorescence Polarization Plate Reader

Sample Injection

• 2x 500 μL injection syringes per batch
• 50 μL dead volumes, 0.5 μL precision
• 25 mL/min maximum injection speed
• 2 mL microcentrifuge tubes, 15 mL conical tubes, or 50 mL conical tube sources

Sample Chamber

• Room temperature (25°C ± 0.45°C (1 sigma)) to 45°C incubation
• Mix by shaking before read options
• 1x microtiter plate (6 to 1,536-well) per batch

Optics

• Xenon flash lamp
• 1 to 100 flashes per well
• High voltage PMT detector
• Top or bottom read mode

Polarization Filters

• Excitation (vertical), Emission (vertical), Emission (horizontal)
  • 485 nm, 520 nm, 520 nm
  • 540 nm, 590 nm, 590 nm
  • 625 nm, 675 nm, 675 nm

Nephelometer

Sample Injection

• 2x 500 µL injection syringes
• 440 µL/s maximum injection speed

Sample Chamber

• Room temperature to 65°C incubation
• Actively regulates O2 and CO2 to 0.1-20%
• Mix by shaking before read options

Optics

• 635 ± 10nm 1mW self-monitoring laser diode
• 1.5 to 3.5 mm selectable beam width
• Side window photodiode detector
• Detects up to 80° full cone angle

Circular Dichroism Plate Reader

• Dual gratings monochromator
• 185 to 880 nm wavelength range (2 nm resolution)
• 96 well plate format
• 2 minute read time for plate (single wavelength)
• 1 hour read time for plate (full spectrum)
• 0.5 L nitrogen gas/min purge
• 45 µL sample volume

Thermal Camera

• Object Temperature Range: -20 to 400°C (-4 to 752°F)
• Accuracy: ±3°C (at 0 – 100°C) and ±3% (at 100 – 400°C)
• Resolution: 5 Megapixels
• Focus: fixed, 0.1m minimum distance
• Image Frequency: 8.7Hz
• Spectral Range: 8 to 14µm

VIS-SWIR InGaAs Camera

• High sensitivity digital VIS-SWIR camera
• Sensor Type: InGaAs PIN-Photodiode
• Active Pixel: 640 x 512
• Pixel Pitch: 15µm x 15µm
• Active Area: 9.6mm x 7.68mm
• Spectral Response: 0.6 to 1.7µm
• Readout Noise (rms): < 50 electrons
• Frame Rate: up to 120Hz
• Exposure Time: 10µs to 26.8s
• Onboard Automated Gain Control (AGC)
• Ultra compact, Low power

Fluorometer

• Signal-to-noise ratio 30,000:1 or better
• Data acquisition rate: 1,000,000 to 1 point/1000 sec
• Inputs: 4 photon counting (TTL); 4 analog (+/- 10 volts) ; 1 analog reference channel (+/- 10 volts); 2 TTL
• Outputs: 2 analog (+/- 10 volts) 2 TTL
• Emission Range: 185 to 900 nm
• Light Source: High efficiency “ECO” friendly continuous
• 75 W Xenon arc lamp
• Monochromator: Coma-aberration corrected, asymmetrical, excitation or emission optimized, Czerny- Turner design
• Focal Length: 350 mm
• Slits: Computer controlled, continuously adjustable
• Emission Grating: 1200 line/mm 400 nm blaze
• Wavelength Accuracy: +/- 0.3 nm
• Minimum Step Size: 0.022 nm
• Detection: Multimode – Photon Counting, 3 analog (fast, medium, slow response), direct and Single-Shot Transient Digitizer (SSTD) mode