SCIENTIFIC INSTRUMENTATION

500 MHz NMR

11.74 T Magnet

 

• 500 MHz for ¹H

• 126 MHz for ¹³C

• 202 MHz for ³¹P

• 470 MHz for ¹⁹F

Probe

 

• SmartProbe ¹H/¹⁹F/¹³C/³¹P 5 mm probe (-150°C to 150°C)

• Inverse Triple Resonance (TXI) ¹H/¹³C/¹⁹N 5 mm probe (-150°C to 150°C)

Autosampler

 

• 6 to 51°C incubation temperature

• 480 NMR tubes per batch

• Standard NMR tubes hold 600 µL to 1.5 mL

• Micro NMR tubes hold 40 µL

2D Experiment Types

 

• Correlation spectroscopy (COSY)

• Double-quantum filtered COSY

• COSY featuring a 45 degree pulse

• Total correlation spectroscopy (TOCSY)

• Heteronuclear single quantum correlation spectroscopy (HSQC, 1H-13C or 1H-15N)

• Heteronuclear multiple quantum correlation spectroscopy (HMQC)

• Heteronuclear multiple-bond correlation spectroscopy (HMBC, 1H-13C or 1H-15N)

• HSQC-TOCSY (1H-13C)

• HMQC-TOCSY (1H-13C)

• Nuclear Overhauser effect spectroscopy (NOESY)

• Rotating-frame Overhauser effect spectroscopy (ROESY)

Microfluidic Spectrometer

Light Source

 

• Xenon flash lamp

Absorbance Detection

 

• 250 to 750 nm wavelength selection

Microfluidics

 

• Fixed path length (0.1 or 0.7 mm) allows for quantification

• Extremely low volumes required (2 μL)

• 96 samples per batch

Multimode Plate Reader w/ Monochromators

Excitation

 

• Xenon flash lamp

• 1 to 100 flashes per well

• 340 to 740 nm excitation monochromator

Emission

 

• High voltage PMT detector

• 340 to 740 nm emission monochromator

• Top or bottom of plate read mode

Sample Chamber

 

• Room temperature (25°C ± 0.45°C (1 sigma)) to 45°C incubation

• Mix by shaking before read options

• 1x microtiter plate (6 to 1,536 well) per batch

Fluorescence Polarization Plate Reader

 

• Xenon flash lamp

• 1 to 100 flashes per well

• Dual high voltage PMT detectors

• 96-well and 384-well plate compatible

• Excitation Emission Filter Pairs

   

  • 485 nm, 520 nm
  • 540 nm, 590 nm
  • 625 nm, 675 nm

• Polarized Dual Simultaneous Emission Filters

FTIR Spectrometer

 

• Deuterated TriGlycine Sulphate (DTGS) Detector

• 375 cm⁻¹ to 7,500 cm⁻¹ spectral range

• > 1,000:1 signal-to-noise ratio

• > 4 cm⁻¹ spectral resolution

• Sample amounts from 20 to 70 µL or 20 to 70 mg required

Multimode Spectrophotometer

Multimode Spectrophotometer

 

• Measurement in sealed quartz capillaries requires only 9 μL of sample

• Measure up to 48 samples in one experiment

• Z-average diameter, polydispersity, isothermal stability, B22, kD, and G22

Dynamic Light Scattering

 

• 660 nm laser

• Avalanche photodiode module scattered light detector

• 0.3 to 1,000 nm hydrodynamic radius range

• < 0.1 polydispersity

• 0.05 mg/mL minimum sample concentration

• 192 Da to 25 MDa molecular weight range

Thermodynamics

 

• Self-contained water-free temperature control system from 15 to 95ºC

• TEC heating/cooling plate temperature control accuracy 1ºC (at < 70ºC) and 1.5ºC (at > 70ºC)

DLS Plate Reader

 

• 830 nm laser

• 0.5 to 1000 nm hydrodynamic radius range

• 1000 to 1 MDa molecular weight range

• 0.125 mg/mL minimum sample concentration

• 4 to 85ºC temperature control

• Support standard 96, 384 or 1,536 microwell plates

• 4 µL minimum sample volume

All Readers

Sample Chamber

 

• Room temperature (25°C ± 0.45°C (1 sigma)) to 45°C incubation

• Mix by shaking before read options

• 1x microtiter plate (6 to 1,536 well) per batch

Sample Injection

 

• 2x 500 µL injection syringes per batch

• 50 µL dead volumes, 0.5 µL precision

• 25 mL/min maximum injection speed

• 2 mL microcentrifuge tubes, 15 mL conical tubes, or 50 mL conical tube sources

Multimode Plate Reader w/ Monochromators

 

• 96-well plate compatible

Excitation

 

• Xenon flash lamp

• 1 to 100 flashes per well

• 340 to 740 nm excitation monochromator

• 680 nm laser (AlphaScreen)

Emission

 

• High voltage PMT detector

• 340 to 740 nm emission monochromator

• Top or bottom of plate read mode

Multimode Plate Reader w/ Filters

 

• 96-well plate compatible

Excitation

 

• Xenon flash lamp

• 1 to 100 flashes per well

• 355 nm, 410 nm, 485 nm, 544 nm, 584 nm, 650 nm, or 700 nm fixed wavelength excitation filters (5 nm bandpass)

Emission

 

• High voltage PMT detector

• 420 nm, 460 nm, 520 nm, 590 nm, 620 nm, or 750 nm fixed wavelength emission filters (5 nm bandpass)

• Top or bottom of plate read mode

Fluorescence Polarization Plate Reader

Sample Injection

 

• 2x 500 μL injection syringes per batch

• 50 μL dead volumes, 0.5 μL precision

• 25 mL/min maximum injection speed

• 2 mL microcentrifuge tubes, 15 mL conical tubes, or 50 mL conical tube sources

Sample Chamber

 

• Room temperature (25°C ± 0.45°C (1 sigma)) to 45°C incubation

• Mix by shaking before read options

• 1x microtiter plate (6 to 1,536-well) per batch

Optics

 

• Xenon flash lamp

• 1 to 100 flashes per well

• High voltage PMT detector

• Top or bottom read mode

Polarization Filters

 

• Excitation (vertical), Emission (vertical), Emission (horizontal)

   

  • 485 nm, 520 nm, 520 nm
  • 540 nm, 590 nm, 590 nm
  • 625 nm, 675 nm, 675 nm

Nephelometer

Sample Injection

 

• 2x 500 µL injection syringes

• 440 µL/s maximum injection speed

Sample Chamber

 

• Room temperature to 65°C incubation

• Actively regulates O2 and CO2 to 0.1-20%

• Mix by shaking before read options

Optics

 

• 635 ± 10nm 1mW self-monitoring laser diode

• 1.5 to 3.5 mm selectable beam width

• Side window photodiode detector

• Detects up to 80° full cone angle

Circular Dichroism Plate Reader

 

• Dual gratings monochromator

• 185 to 880 nm wavelength range (2 nm resolution)

• 96 well plate format

• 2 minute read time for plate (single wavelength)

• 1 hour read time for plate (full spectrum)

• 0.5 L nitrogen gas/min purge

• 45 µL sample volume