Cell Culture

Workcell Integrated Micro Liquid Handler

8x Independent Single Channel Pipettes

• 1 μL to 1 mL volume (± 1%)
• 1 to 500 µL/sec aspirate and dispense rate
• Disposable polypropylene filter tips

1x Multichannel Pipette (96 Channels)

• Capable of any simultaneous rectangular pipetting up to dimensions 8×12
• 1 μL to 1 mL volume (± 2%)
• 1 to 500 µL/sec aspirate/dispense rate
• Disposable polypropylene filter tips

Large Capacity Deck

• 4x SBS tilt-plate positions (for media aspiration)
• 1x Heating/Cooling/Shaking SBS plate position
  • 100 to 2,000 RPM shaking rate
  • Orbital or linear shaking patterns available
  • 4 to 70°C temperature incubation
• 5x incubated plate positions (-15 to 95°C)
• 7x SBS plate positions
• 4x 200 mL reagent trough positions
• 12x 50 mL tube positions
• 64x microcentrifuge tube positions
• Robotic arm for movement of plates, lids, and racks around the deck and to/from integrations
• Vacuum block for filtering
  • SBS microtiter filter plate source
  • SBS collection plate (deep well)
  • Source and destination plate movable

Workcell Integrated Incubator

• Temperature range: 33 to 50°C
• Ambient to 95% relative humidity
• 0.04 to 20% CO2

Workcell Integrated High Speed Centrifuge

• 5,700 RPM maximum speed (4,000 x g at Rmax)
• 350 g maximum weight (per position)
• 50 g maximum imbalance
• 2x SBS plate footprint positions

Workcell Integrated Confocal High Content Imager

• Field of view (10x magnification): 1 96 mm2
• Speed: Up to 200,000 wells/day
• Objectives: 1x to 100x air and oil
• Filter changer: 5 position filter cube changer
• Autofocus: Laser and image autofocus
• Fully automated X-Y and Z stages with resolution better than 100 nm”
• Environmental control

Imaging Modes

• Digital phase contrast
• Transmitted light
• Brightfield
• Fluorescence
• Sample Constraints

Sample Constraints

• Up to 1,536-well plates

Workcell Integrated Positive Pressure Filter

• 0 to 40 PSI independent pressure sources for each well
• Filter though SBS filter plates
• Collects in SBS deep well plates

Workcell Integrated Plate Reader

• Supports absorbance, fluorescence, and luminescence reads

Absorbance

• CCD detector
• 220 to 1,000 nm wavelength selection

Excitation

• Xenon flash lamp
• 1 to 100 flashes per well
• 320 to 740 nm excitation monochromator (8 nm bandpass)
• 680 nm laser (AlphaScreen)

Emission

• High voltage PMT detector
• 320 to 850 nm emission monochromator (8 nm bandpass)

Sample Injection

• 2x 500 µL injection syringes per batch
• 50 µL dead volumes, 0.5 µL precision
• 25 mL/min maximum injection speed
• 2 mL microcentrifuge tubes, 15 mL conical tubes, or 50 mL conical tube sources

Sample Chamber

• Room temperature (25°C ± 0.45°C (1 sigma)) to 45°C incubation
• Mix by shaking before read options
• 1x microtiter plate (6 to 96 well) per batch

Nephelometer

Sample Injection

• 2x 500 µL injection syringes
• 440 µL/s maximum injection speed

Sample Chamber

• Room temperature to 60°C incubation
• Actively regulates O2 and CO2 to 0.1-20%
• Mix by shaking before read options
• 1x microtiter plate (up to 384-well) per batch

Optics

• 635 ± 10nm 1mW self-monitoring laser diode
• 1.5 to 3.5 mm selectable beam width
• Side window photodiode detector
• Detects up to 80° full cone angle

Automated Thermal Cycler

• Temperature accuracy: ± 0.25°C (between 35.0 and 99.9°C)
• Max block ramp rate: 3.5°C/sec
• Max sample ramp rate: 1.8°C/sec
• Temperature range: 4 to 105°C
• Temperature uniformity: ± 0.50°C (20 sec after reaching 95°C)
• Sample Constraints

Sample Constraints

• Plate format: 96-well PCR plate
• Sample volume: 10 to 100 µL

Incubator Shaker

• 400 to 1800 RPM
• Programmable shaking pattern
• 1x deep well or 1x microtiter plate or 24x 2ml tubes
• Incubation at temperatures between ambient and 80ºC

Flow Cytometer

Fluidics

• 0.025 to 3 5 uLisec sample fow rate

Optics

• 5- spatially separated lasers
• 355 nm, 50 mW
• 405 nm, 100 mW
• 488 nm, 100 mW
• 561 nm, 50 mW
• 640 nm, 100 mW

Detectors

• 30 PMT detectors including 2 FSC and 1 SSC
• 100,000 eps maximal acquisition rate with all parameters engaged

FSC Particle Size Resolution

• FITC: 70 MESF, PE: 50 MESF, APC: 40 MESF

Sample Formats

• 5mL tube, microcentrifuge tube, 96-well standard/ deep well plates, 384-well standard/deep well plates.

Sample Environmenal Control

• Sample agitation and temperature control between 18 to 28ºC

High-throughput Sampling Speed

• 15 minutes for 96-well plate
• 60 minutes for 384-well plate

Plate Based Electroporator

• 5 to 300 V range
• 5% voltage delivery accuracy
• Square wave and exponential decay pulse mode options
• Pulse delivery
  • 10 µs to 999 ms pulse length range
  • 1 to 99 pulses per sample
  • 0.1 to 10 sec pulse intervals
  • 10 to 3,775 µF capacitance, dependent on pulse mode and voltage
• 50 to 1,000 Ω in 50 Ω steps
• 96-well plate with either 100 µL or 200 µL well options

Controlled Rate Cell Freezing Apparatus

• Maximum cooling rate 1°C per minute
• Minimum temperature -100°C
• Programmable temperature profile
• Temperature monitoring data recorded during the run
• Sample Constraints
• 48x cryotubes (2 mL) maximum per batch

Controlled Rate Cell Thawing Apparatus

• 37°C heating element
• ~3 min thawing times

Sample Constraints

• 1x cryotube (2 mL) maximum per batch

Workcell Integrated Incubator

Pouring Capabilities

• Fills up to 50 plates with agar every 6 minutes
• 3% volumetric pouring accuracy
• 480 mL/min maximum flow rate

Decontamination Features

• Integrated UV lamp for decontamination
• Autoclavable internals such as pouring tubes
• 2x cabinet HEPA filter and 1x vent HEPA filters

Sample Constraints

• Petri dish (90 mm & 150 mm)
• SBS standard rectangular format
• Square dish (120 mm)

Robotic Multi-Parallel Bioreactors

• Integrated, automated liquid handling for all liquid samples and additions
• Up to 12 bioreactor stations
• Three gases per bioreactor (O2, CO2, N2, Air)
• Four positive displacement liquid pumps per bioreactor
• Individual bioreactor temperature control with heating or cooling
• Individual bioreactor impeller speed control

Vessels

• 100 to 250 mL working volume with baffes
• Dual 20 mm pitched-blade or Rushton impeller
• Spot-based DO sensor
• Disposable pH electrode
• Integrated gas and liquid inlet filters
• Robotic compatible cap for sampling

Large Lab Bioreactor

• Temperature range: 0 to 40°C
• Maximum relative humidity: 80% (up to 31°C), decreasing to 50% (40°C)
• Motor speed ranges:
  • 1 L glass: 30 to 1,400 RPM
  • 2 L glass: 30 to 1,100 RPM
  • 5 L glass: 30 to 800 RPM
  • 2 L single-use: 30 to 400 RPM

Liquid Handling

• 3 pumps
• Fixed speed: 43 RPM
• Flow rate: 0.8 mL/min (ID 0.5 mm) up to 20.2 mL/min (ID 3.2 mm)

Probes and Controllers

• Glass vessels
  • Temperature sensor (pH probe integrated)
    • Control range: 0 to 60°C
    • Display resolution: 0.1°C
  • DO probe (reusable)
    • Polarographic probe
    • Digital communication with Control Tower
    • Range: 0 to 100%
    • Display resolution: 0.1%
  • pH probe (reusable)
    • Combination electrode
    • Range: 2 to 12 pH
    • Display resolution: 0.01 pH
  • Foam, alternative level
    • Electrical conductivity sensor
• Single-use vessels
  • Temperature sensor (standalone)
    • Control range: 0 to 40°C
    • Display resolution: 0.1°C
  • DO sensor (single-use)
    • Sensor patch
    • Range: 0 to 100% air saturation
    • Display resolution: 0.1% air saturation
  • pH sensor (single-use)
    • Sensor patch
    • Range: 6 to 8 pH
    • Display resolution: 0.1 pH

Aeration

• Two-gas (microbial)
  • Continuous automatic aeration control for air and O2
  • Flow rates: 100 to 7,500 ccm
  • Flow accuracy: ± 5% full scale
• Four-gas (cell culture)
  • Continuous automatic aeration control for air, O2, N2, CO2
  • Flow rates: 10 to 550 ccm (air and N2); 5 to 250 ccm (O2 and CO2)
  • Flow accuracy: ± 5% full scale

Cooling

• Supply temperature: 8°C ± 2°C at ambient temperature

Plate-Based Bioreactor

Cultivation Conditions

• Temperature control: 10 to 50ºC
• Controlled gas atmosphere in headspace: CO2 (0 to 12%) and O2 (1 to 100%)
• Plate shaking (100 to 1,500 RPM)
• Well-specific pH control
• Well-specific feeding: constant, linear, exponential, or signal-triggered

Sensors

• Dissolved Oxygen (0 to 100%)
• pH (4 to 7.5)
• Biomass concentration (equivalent to up to 250 OD600)
• Fluorescence
• NAD(P)H and ribofavins

Plate Types (All Disposable)

• FlowerPlate: 48 wells, 800 to 1,900 µL, high oxygen transfer rate and oxygen supply efficiency
• Round well plates: 48 wells, 100 to 2,400 µL, low sheer force
• Microfuidic plates (fower or round): 32 cultivation wells with 16 reservoir plates

Confocal High Content Imager

• Camera: > 4.7 megapixel CMOS
• Field of view (10x magnification): 1 96 mm2
• Speed: Up to 200,000 wells/day
• Objectives: 4x, 10x, 20x (water immersion) and 60x (water immersion)
• 8 position emission wheel and 5 position dichroic filter changer
• Autofocus: laser and image autofocus
• Fully automated X-Y and Z stages with resolution better than 100 nm
• Full humidity, CO2 and temperature control

Imaging Modes

• Confocal & Widefield
  • Digital phase contrast
  • Bright-field
  • Fluorescence

Sampling Constraints

• Up to 1,536-well plates

Robotic Colony Picker

• 3,000 colonies picked per hour (brightfield) and 2,000 per hour (fuorescence)
• Capable of acquiring bright field (trans-illuminated) or fuorescent images to identify colony positions

Fluorescent Filtering Options

• 377 nm excitation filtering with 447 nm emission filtering (good for DAP iHoechst)
• 457 nm excitation filtering with 536 nm emission filtering (good for F TCiGFP)
• 531 nm excitation filtering with 593 nm emission filtering (good for Cy3iDS Red)
• 531 nm excitation filtering with 624 nm emission filtering (good for RhodamineiTexas Red)
• 628 nm excitation filtering with 692 nm emission filtering (good for Cy5)

Imaging

• Imaging
• Colony image and fuorescence intensity data available for each colony picked (5.3 megapixel images)

Sample Constraints

• 2x source solid media plates per batch
• 16x SBS deep well plate destination positions for liquid media

Plate Based Electroporator

• 5 to 300 V range
• 5% voltage delivery accuracy
• Square wave and exponential decay pulse mode options
• Pulse delivery
  • 10 µs to 999 ms pulse length range
  • 1 to 99 pulses per sample
  • 0.1 to 10 sec pulse intervals
  • 10 to 3,775 µF capacitance, dependent on pulse mode and voltage
• 50 to 1,000 O in 50 O steps
• 96-well plate with either 100 µL or 200 µL well options

Flow Cytometer

Fluidics

• 025 to 3 5 uLisec sample fow rate

Optics

• 5- spatially separated lasers
• 355 nm, 50 mW
• 405 nm, 100 mW
• 488 nm, 100 mW
• 561 nm, 50 mW
• 640 nm, 100 mW

Detectors

• 30 PMT detectors including 2 FSC and 1 SSC
• 100,000 eps maximal acquisition rate with all parameters engaged

FSC Particle Size Resolution

• FSC Particle Size Resolution
• 0.2 um

Fluorescence Sensitivity

• FITC: 70 MESF, PE: 50 MESF, APC: 40 MESF

Sample Formats

• 5mL tube, microcentrifuge tube, 96-well standard/ deep well plates, 384-well standard/deep well plates.

Sample Environmental Control

• Sample agitation and temperature control between 18 to 28ºC

High-throughput Sampling Speed

• 15 minutes for 96-well plate
• 60 minutes for 384-well plate

Fluorescence Activated Cell Sorter

Sorting Technology

• Instrument supports conventional fow cytometry based sorting with compensation controls or spectral fow cytometry based sorting

Sensitivity

• < 100 MESF for FITC, PE, APC
• < 0.2 µm FSC resolution with small particle detection module

Sample Input

• 6 tube input positions for 1.5mL, 5mL, or 15mL tubes
• Temperature control (4 to 37°C) and agitation for all positions

Sort Output

• Up to 6-way sorting into tubes
• Configurable tube holders include 1 5, 5, 15, and 50mL adapters
• Multi-way micro-well plate sorting up to 1,536 wells

Temperature Control

• 4 to 37°C for all media types.

Nozzle

• 50, 70, 100, 120, and 150µm ceramic nozzle tips with adjustable pressure settings

Biocontainment

• Integrated Class II biocontainment cabinet for protection of sample and user
• Separate AES for sort chamber evacuation

Optics

• Excitation: seven lasers (all 100mW)
• 349, 405, 445, 488, 561, 640, and 785nm
• Detection: Up to 60 parameters including FSC and SSC

Workcell Integrated Incubator

• Temperature range: 33 to 50°C
• Ambient to 95% relative humidity
• 0.04 to 20% CO2

Workcell Integrated Incubator

• Temperature range: 33 to 50°C
• Ambient to 95% relative humidity
• 0.04 to 20% CO2

Workcell Integrated Incubator

• Temperature range: 33 to 50°C
• Ambient to 95% relative humidity
• 0.04 to 20% CO2