SCIENTIFIC INSTRUMENTATION


CELL CULTURE

ExperimentCellHandling[]

Robotic Cell Preparation Workcell

Workcell Integrated Micro Liquid Handler

8x Independent Single Channel Pipettes

  • 1 μL to 1 mL volume (± 1%)
  • 1 to 500 µL/sec aspirate and dispense rate
  • Disposable polypropylene filter tips

1x Multichannel Pipette (96 Channels)

  • Capable of any simultaneous rectangular pipetting up to dimensions 8×12
  • 1 μL to 1 mL volume (± 2%)
  • 1 to 500 µL/sec aspirate/dispense rate
  • Disposable polypropylene filter tips

Large Capacity Deck

  • 4x SBS tilt-plate positions (for media aspiration)
  • 1x Heating/Cooling/Shaking SBS plate position
    • 100 to 2,000 RPM shaking rate
    • Orbital or linear shaking patterns available
    • 4 to 70°C temperature incubation
  • 5x incubated plate positions (-15 to 95°C)
  • 7x SBS plate positions
  • 4x 200 mL reagent trough positions
  • 12x 50 mL tube positions
  • 64x microcentrifuge tube positions
  • Robotic arm for movement of plates, lids, and racks around the deck and to/from integrations
  • Vacuum block for filtering
    • SBS microtiter filter plate source
    • SBS collection plate (deep well)
    • Source and destination plate movable

Workcell Integrated Incubator

  • Temperature range: 33 to 50°C
  • Ambient to 95% relative humidity
  • 0.04 to 20% CO2
Liconic STX44-ICBT

Workcell Integrated High Speed Centrifuge

  • 5,700 RPM maximum speed (4,000 x g at Rmax)
  • 350 g maximum weight (per position)
  • 50 g maximum imbalance
  • 2x SBS plate footprint positions
Bionex HIG4

Workcell Integrated Confocal High Content Imager

  • Field of view (10x magnification): 1 96 mm2
  • Speed: Up to 200,000 wells/day
  • Objectives: 1x to 100x air and oil
  • Filter changer: 5 position filter cube changer
  • Autofocus: Laser and image autofocus
  • Fully automated X-Y and Z stages with resolution better than 100 nm”
  • Environmental control

Imaging Modes

  • Digital phase contrast
  • Transmitted light
  • Brightfield
  • Fluorescence
  • Sample Constraints

Sample Constraints

  • Up to 1,536-well plates
Imagexpress Micro Confocal

Workcell Integrated Positive Pressure Filter

  • 0 to 40 PSI independent pressure sources for each well
  • Filter though SBS filter plates
  • Collects in SBS deep well plates
Hamiilton MPE2

Workcell Integrated Plate Reader

  • Supports absorbance, fluorescence, and luminescence reads

Absorbance

  • CCD detector
  • 220 to 1,000 nm wavelength selection

Excitation

  • Xenon flash lamp
  • 1 to 100 flashes per well
  • 320 to 740 nm excitation monochromator (8 nm bandpass)
  • 680 nm laser (AlphaScreen)

Emission

  • High voltage PMT detector
  • 320 to 850 nm emission monochromator (8 nm bandpass)

Sample Injection

  • 2x 500 µL injection syringes per batch
  • 50 µL dead volumes, 0.5 µL precision
  • 25 mL/min maximum injection speed
  • 2 mL microcentrifuge tubes, 15 mL conical tubes, or 50 mL conical tube sources

Sample Chamber

  • Room temperature (25°C ± 0.45°C (1 sigma)) to 45°C incubation
  • Mix by shaking before read options
  • 1x microtiter plate (6 to 96 well) per batch

Nephelometer

Sample Injection

  • 2x 500 µL injection syringes
  • 440 µL/s maximum injection speed

Sample Chamber

  • Room temperature to 60°C incubation
  • Actively regulates O2 and CO2 to 0.1-20%
  • Mix by shaking before read options
  • 1x microtiter plate (up to 384-well) per batch

Optics

  • 635 ± 10nm 1mW self-monitoring laser diode
  • 1.5 to 3.5 mm selectable beam width
  • Side window photodiode detector
  • Detects up to 80° full cone angle

Automated Thermal Cycler

  • Temperature accuracy: ± 0.25°C (between 35.0 and 99.9°C)
  • Max block ramp rate: 3.5°C/sec
  • Max sample ramp rate: 1.8°C/sec
  • Temperature range: 4 to 105°C
  • Temperature uniformity: ± 0.50°C (20 sec after reaching 95°C)
  • Sample Constraints

Sample Constraints

  • Plate format: 96-well PCR plate
  • Sample volume: 10 to 100 µL

Incubator Shaker

  • 400 to 1800 RPM
  • Programmable shaking pattern
  • 1x deep well or 1x microtiter plate or 24x 2ml tubes
  • Incubation at temperatures between ambient and 80ºC
Inheco Shaker

Flow Cytometer

Fluidics

  • 0.025 to 3 5 uLisec sample fow rate

Optics

  • 5- spatially separated lasers
  • 355 nm, 50 mW
  • 405 nm, 100 mW
  • 488 nm, 100 mW
  • 561 nm, 50 mW
  • 640 nm, 100 mW

Detectors

  • 30 PMT detectors including 2 FSC and 1 SSC
  • 100,000 eps maximal acquisition rate with all parameters engaged

FSC Particle Size Resolution

  • FITC: 70 MESF, PE: 50 MESF, APC: 40 MESF

Sample Formats

  • 5mL tube, microcentrifuge tube, 96-well standard/ deep well plates, 384-well standard/deep well plates.

Sample Environmenal Control

  • Sample agitation and temperature control between 18 to 28ºC

High-throughput Sampling Speed

  • 15 minutes for 96-well plate
  • 60 minutes for 384-well plate
Biorad Ze5 Cell Analyzer

Plate Based Electroporator

  • 5 to 300 V range
  • 5% voltage delivery accuracy
  • Square wave and exponential decay pulse mode options
  • Pulse delivery
    • 10 µs to 999 ms pulse length range
    • 1 to 99 pulses per sample
    • 0.1 to 10 sec pulse intervals
    • 10 to 3,775 µF capacitance, dependent on pulse mode and voltage
  • 50 to 1,000 Ω in 50 Ω steps
  • 96-well plate with either 100 µL or 200 µL well options
Lonza 4D

ExperimentFreezeCells[]

Controlled Rate Cell Freezing Apparatus

  • Maximum cooling rate 1°C per minute
  • Minimum temperature -100°C
  • Programmable temperature profile
  • Temperature monitoring data recorded during the run
  • Sample Constraints
  • 48x cryotubes (2 mL) maximum per batch
Asymptote Via Freeze

ExperimentThawCells[]

Controlled Rate Cell Thawing Apparatus

  • 37°C heating element
  • ~3 min thawing times

Sample Constraints

  • 1x cryotube (2 mL) maximum per batch
Thawstar

ExperimentPourPlates[]

Workcell Integrated Incubator

Pouring Capabilities

  • Fills up to 50 plates with agar every 6 minutes
  • 3% volumetric pouring accuracy
  • 480 mL/min maximum flow rate

Decontamination Features

  • Integrated UV lamp for decontamination
  • Autoclavable internals such as pouring tubes
  • 2x cabinet HEPA filter and 1x vent HEPA filters

Sample Constraints

  • Petri dish (90 mm & 150 mm)
  • SBS standard rectangular format
  • Square dish (120 mm)
Singer Instruments Serial Filler
Singer Instruments Serial Filler

ExperimentBioreactor[]

Robotic Multi-Parallel Bioreactors

  • Integrated, automated liquid handling for all liquid samples and additions
  • Up to 12 bioreactor stations
  • Three gases per bioreactor (O2, CO2, N2, Air)
  • Four positive displacement liquid pumps per bioreactor
  • Individual bioreactor temperature control with heating or cooling
  • Individual bioreactor impeller speed control

Vessels

  • 100 to 250 mL working volume with baffes
  • Dual 20 mm pitched-blade or Rushton impeller
  • Spot-based DO sensor
  • Disposable pH electrode
  • Integrated gas and liquid inlet filters
  • Robotic compatible cap for sampling
Sartorius Amr 250

Large Lab Bioreactor

  • Temperature range: 0 to 40°C
  • Maximum relative humidity: 80% (up to 31°C), decreasing to 50% (40°C)
  • Motor speed ranges:
    • 1 L glass: 30 to 1,400 RPM
    • 2 L glass: 30 to 1,100 RPM
    • 5 L glass: 30 to 800 RPM
    • 2 L single-use: 30 to 400 RPM

Liquid Handling

  • 3 pumps
  • Fixed speed: 43 RPM
  • Flow rate: 0.8 mL/min (ID 0.5 mm) up to 20.2 mL/min (ID 3.2 mm)

Probes and Controllers

  • Glass vessels
    • Temperature sensor (pH probe integrated)
      • Control range: 0 to 60°C
      • Display resolution: 0.1°C
    • DO probe (reusable)
      • Polarographic probe
      • Digital communication with Control Tower
      • Range: 0 to 100%
      • Display resolution: 0.1%
    • pH probe (reusable)
      • Combination electrode
      • Range: 2 to 12 pH
      • Display resolution: 0.01 pH
    • Foam, alternative level
      • Electrical conductivity sensor
  • Single-use vessels
    • Temperature sensor (standalone)
      • Control range: 0 to 40°C
      • Display resolution: 0.1°C
    • DO sensor (single-use)
      • Sensor patch
      • Range: 0 to 100% air saturation
      • Display resolution: 0.1% air saturation
    • pH sensor (single-use)
      • Sensor patch
      • Range: 6 to 8 pH
      • Display resolution: 0.1 pH

Aeration

  • Two-gas (microbial)
    • Continuous automatic aeration control for air and O2
    • Flow rates: 100 to 7,500 ccm
    • Flow accuracy: ± 5% full scale
  • Four-gas (cell culture)
    • Continuous automatic aeration control for air, O2, N2, CO2
    • Flow rates: 10 to 550 ccm (air and N2); 5 to 250 ccm (O2 and CO2)
    • Flow accuracy: ± 5% full scale

Cooling

  • Supply temperature: 8°C ± 2°C at ambient temperature
Sartorius Biostat A
Sartorius Biostat A

Plate-Based Bioreactor

Cultivation Conditions

  • Temperature control: 10 to 50ºC
  • Controlled gas atmosphere in headspace: CO2 (0 to 12%) and O2 (1 to 100%)
  • Plate shaking (100 to 1,500 RPM)
  • Well-specific pH control
  • Well-specific feeding: constant, linear, exponential, or signal-triggered

Sensors

  • Dissolved Oxygen (0 to 100%)
  • pH (4 to 7.5)
  • Biomass concentration (equivalent to up to 250 OD600)
  • Fluorescence
  • NAD(P)H and ribofavins

Plate Types (All Disposable)

  • FlowerPlate: 48 wells, 800 to 1,900 µL, high oxygen transfer rate and oxygen supply efficiency
  • Round well plates: 48 wells, 100 to 2,400 µL, low sheer force
  • Microfuidic plates (fower or round): 32 cultivation wells with 16 reservoir plates
BeckmanCoulter/M2P Labs Bioector XT
Beckman Coulter – BioLector Pro

ExperimentImageCells[]

Confocal High Content Imager

  • Camera: > 4.7 megapixel CMOS
  • Field of view (10x magnification): 1 96 mm2
  • Speed: Up to 200,000 wells/day
  • Objectives: 4x, 10x, 20x (water immersion) and 60x (water immersion)
  • 8 position emission wheel and 5 position dichroic filter changer
  • Autofocus: laser and image autofocus
  • Fully automated X-Y and Z stages with resolution better than 100 nm
  • Full humidity, CO2 and temperature control

Imaging Modes

  • Confocal & Widefield
    • Digital phase contrast
    • Bright-field
    • Fluorescence

Sampling Constraints

  • Up to 1,536-well plates

ExperimentColonyPicking[]

ExperimentSpreadPlating[]

Robotic Colony Picker

  • 3,000 colonies picked per hour (brightfield) and 2,000 per hour (fuorescence)
  • Capable of acquiring bright field (trans-illuminated) or fuorescent images to identify colony positions

Fluorescent Filtering Options

  • 377 nm excitation filtering with 447 nm emission filtering (good for DAP iHoechst)
  • 457 nm excitation filtering with 536 nm emission filtering (good for F TCiGFP)
  • 531 nm excitation filtering with 593 nm emission filtering (good for Cy3iDS Red)
  • 531 nm excitation filtering with 624 nm emission filtering (good for RhodamineiTexas Red)
  • 628 nm excitation filtering with 692 nm emission filtering (good for Cy5)

Imaging

  • Imaging
  • Colony image and fuorescence intensity data available for each colony picked (5.3 megapixel images)

Sample Constraints

  • 2x source solid media plates per batch
  • 16x SBS deep well plate destination positions for liquid media
qpix 420 HT

ExperimentElectroporation[]

Plate Based Electroporator

  • 5 to 300 V range
  • 5% voltage delivery accuracy
  • Square wave and exponential decay pulse mode options
  • Pulse delivery
    • 10 µs to 999 ms pulse length range
    • 1 to 99 pulses per sample
    • 0.1 to 10 sec pulse intervals
    • 10 to 3,775 µF capacitance, dependent on pulse mode and voltage
  • 50 to 1,000 O in 50 O steps
  • 96-well plate with either 100 µL or 200 µL well options

ExperimentFlowCytometry[]

Flow Cytometer

Fluidics

  • 025 to 3 5 uLisec sample fow rate

Optics

  • 5- spatially separated lasers
  • 355 nm, 50 mW
  • 405 nm, 100 mW
  • 488 nm, 100 mW
  • 561 nm, 50 mW
  • 640 nm, 100 mW

Detectors

  • 30 PMT detectors including 2 FSC and 1 SSC
  • 100,000 eps maximal acquisition rate with all parameters engaged

FSC Particle Size Resolution

  • FSC Particle Size Resolution
  • 0.2 um

Fluorescence Sensitivity

  • FITC: 70 MESF, PE: 50 MESF, APC: 40 MESF

Sample Formats

  • 5mL tube, microcentrifuge tube, 96-well standard/ deep well plates, 384-well standard/deep well plates.

Sample Environmental Control

  • Sample agitation and temperature control between 18 to 28ºC

High-throughput Sampling Speed

  • 15 minutes for 96-well plate
  • 60 minutes for 384-well plate

ExperimentFACS[]

Fluorescence Activated Cell Sorter

Sorting Technology

  • Instrument supports conventional fow cytometry based sorting with compensation controls or spectral fow cytometry based sorting

Sensitivity

  • < 100 MESF for FITC, PE, APC
  • < 0.2 µm FSC resolution with small particle detection module

Sample Input

  • 6 tube input positions for 1.5mL, 5mL, or 15mL tubes
  • Temperature control (4 to 37°C) and agitation for all positions

Sort Output

  • Up to 6-way sorting into tubes
  • Configurable tube holders include 1 5, 5, 15, and 50mL adapters
  • Multi-way micro-well plate sorting up to 1,536 wells

Temperature Control

  • 4 to 37°C for all media types.

Nozzle

  • 50, 70, 100, 120, and 150µm ceramic nozzle tips with adjustable pressure settings

Biocontainment

  • Integrated Class II biocontainment cabinet for protection of sample and user
  • Separate AES for sort chamber evacuation

Optics

  • Excitation: seven lasers (all 100mW)
  • 349, 405, 445, 488, 561, 640, and 785nm
  • Detection: Up to 60 parameters including FSC and SSC
Propel Labs Bigfoot

Workcell Integrated Incubator

  • Temperature range: 33 to 50°C
  • Ambient to 95% relative humidity
  • 0.04 to 20% CO2
Liconic STX44-ICBT

Workcell Integrated Incubator

  • Temperature range: 33 to 50°C
  • Ambient to 95% relative humidity
  • 0.04 to 20% CO2
Liconic STX44-ICBT

Workcell Integrated Incubator

  • Temperature range: 33 to 50°C
  • Ambient to 95% relative humidity
  • 0.04 to 20% CO2
Liconic STX44-ICBT