Cell Culture

Workcell Integrated Micro Liquid Handler

8x Independent Single Channel Pipettes

1 μL to 1 mL volume (± 1%)

1 to 500 µL/sec aspirate and dispense rate

Disposable polypropylene filter tips

1x Multichannel Pipette (96 Channels)

Capable of any simultaneous rectangular pipetting up to dimensions 8×12

1 μL to 1 mL volume (± 2%)

1 to 500 µL/sec aspirate/dispense rate

Disposable polypropylene filter tips

Large Capacity Deck

4x SBS tilt-plate positions (for media aspiration)

1x Heating/Cooling/Shaking SBS plate position

5x incubated plate positions (-15 to 95°C)

7x SBS plate positions

4x 200 mL reagent trough positions

12x 50 mL tube positions

64x microcentrifuge tube positions

Robotic arm for movement of plates, lids, and racks around the deck and to/from integrations

Vacuum block for filtering

Workcell Integrated Incubator

Temperature range: 33 to 50°C

Ambient to 95% relative humidity

0.04 to 20% CO2

Workcell Integrated High Speed Centrifuge

5,700 RPM maximum speed (4,000 x g at Rmax)

350 g maximum weight (per position)

50 g maximum imbalance

2x SBS plate footprint positions

Workcell Integrated Confocal High Content Imager

Field of view (10x magnification): 1 96 mm2

Speed: Up to 200,000 wells/day

Objectives: 1x to 100x air and oil

Filter changer: 5 position filter cube changer

Autofocus: Laser and image autofocus

Fully automated X-Y and Z stages with resolution better than 100 nm”

Environmental control

Imaging Modes

Digital phase contrast

Transmitted light

Brightfield

Fluorescence

Sample Constraints

Sample Constraints

Up to 1,536-well plates

Workcell Integrated Positive Pressure Filter

0 to 40 PSI independent pressure sources for each well

Filter though SBS filter plates

Collects in SBS deep well plates

Workcell Integrated Plate Reader

Supports absorbance, fluorescence, and luminescence reads

Absorbance

CCD detector

220 to 1,000 nm wavelength selection

Excitation

Xenon flash lamp

1 to 100 flashes per well

320 to 740 nm excitation monochromator (8 nm bandpass)

680 nm laser (AlphaScreen)

Emission

High voltage PMT detector

320 to 850 nm emission monochromator (8 nm bandpass)

Sample Injection

2x 500 µL injection syringes per batch

50 µL dead volumes, 0.5 µL precision

25 mL/min maximum injection speed

2 mL microcentrifuge tubes, 15 mL conical tubes, or 50 mL conical tube sources

Sample Chamber

Room temperature (25°C ± 0.45°C (1 sigma)) to 45°C incubation

Mix by shaking before read options

1x microtiter plate (6 to 96 well) per batch

Nephelometer

Sample Injection

2x 500 µL injection syringes

440 µL/s maximum injection speed

Sample Chamber

Room temperature to 60°C incubation

Actively regulates O2 and CO2 to 0.1-20%

Mix by shaking before read options

1x microtiter plate (up to 384-well) per batch

Optics

635 ± 10nm 1mW self-monitoring laser diode

1.5 to 3.5 mm selectable beam width

Side window photodiode detector

Detects up to 80° full cone angle

Automated Thermal Cycler

Temperature accuracy: ± 0.25°C (between 35.0 and 99.9°C)

Max block ramp rate: 3.5°C/sec

Max sample ramp rate: 1.8°C/sec

Temperature range: 4 to 105°C

Temperature uniformity: ± 0.50°C (20 sec after reaching 95°C)

Sample Constraints

Sample Constraints

Plate format: 96-well PCR plate

Sample volume: 10 to 100 µL

Incubator Shaker

400 to 1800 RPM

Programmable shaking pattern

1x deep well or 1x microtiter plate or 24x 2ml tubes

Incubation at temperatures between ambient and 80ºC

Controlled Rate Cell Freezing Apparatus

Maximum cooling rate 1°C per minute

Minimum temperature -100°C

Programmable temperature profile

Temperature monitoring data recorded during the run

Sample Constraints

48x cryotubes (2 mL) maximum per batch

Controlled Rate Cell Thawing Apparatus

37°C heating element

~3 min thawing times

Sample Constraints

1x cryotube (2 mL) maximum per batch

Plate-Based Bioreactor

Cultivation Conditions

Temperature control: 10 to 50ºC

Controlled gas atmosphere in headspace: CO2 (0 to 12%) and O2 (1 to 100%)

Plate shaking (100 to 1,500 RPM)

Well-specific pH control

Well-specific feeding: constant, linear, exponential, or signal-triggered

Sensors

Dissolved Oxygen (0 to 100%)

pH (4 to 7.5)

Biomass concentration (equivalent to up to 250 OD600)

Fluorescence

NAD(P)H and ribofavins

Plate Types (All Disposable)

FlowerPlate: 48 wells, 800 to 1,900 µL, high oxygen transfer rate and oxygen supply efficiency

Round well plates: 48 wells, 100 to 2,400 µL, low sheer force

Microfuidic plates (fower or round): 32 cultivation wells with 16 reservoir plates

Confocal High Content Imager

Camera: > 4.7 megapixel CMOS

Field of view (10x magnification): 1 96 mm2

Speed: Up to 200,000 wells/day

Objectives: 4x, 10x, 20x (water immersion) and 60x (water immersion)

8 position emission wheel and 5 position dichroic filter changer

Autofocus: laser and image autofocus

Fully automated X-Y and Z stages with resolution better than 100 nm

Full humidity, CO2 and temperature control

Imaging Modes

Confocal & Widefield

Sampling Constraints

Up to 1,536-well plates

Robotic Colony Picker

3,000 colonies picked per hour (brightfield) and 2,000 per hour (fuorescence)

Capable of acquiring bright field (trans-illuminated) or fuorescent images to identify colony positions

Fluorescent Filtering Options

377 nm excitation filtering with 447 nm emission filtering (good for DAP iHoechst)

457 nm excitation filtering with 536 nm emission filtering (good for F TCiGFP)

531 nm excitation filtering with 593 nm emission filtering (good for Cy3iDS Red)

531 nm excitation filtering with 624 nm emission filtering (good for RhodamineiTexas Red)

628 nm excitation filtering with 692 nm emission filtering (good for Cy5)

Imaging

Imaging

Colony image and fuorescence intensity data available for each colony picked (5.3 megapixel images)

Sample Constraints

2x source solid media plates per batch

16x SBS deep well plate destination positions for liquid media

Fluorescence Activated Cell Sorter

Sorting Technology

Instrument supports conventional fow cytometry based sorting with compensation controls or spectral fow cytometry based sorting

Sensitivity

< 100 MESF for FITC, PE, APC

< 0.2 µm FSC resolution with small particle detection module

Sample Input

6 tube input positions for 1.5mL, 5mL, or 15mL tubes

Temperature control (4 to 37°C) and agitation for all positions

Sort Output

Up to 6-way sorting into tubes

Configurable tube holders include 1 5, 5, 15, and 50mL adapters

Multi-way micro-well plate sorting up to 1,536 wells

Temperature Control

4 to 37°C for all media types.

Nozzle

50, 70, 100, 120, and 150µm ceramic nozzle tips with adjustable pressure settings

Biocontainment

Integrated Class II biocontainment cabinet for protection of sample and user

Separate AES for sort chamber evacuation

Optics

Excitation: seven lasers (all 100mW)

349, 405, 445, 488, 561, 640, and 785nm

Detection: Up to 60 parameters including FSC and SSC

Workcell Integrated Incubator

Temperature range: 33 to 50°C

Ambient to 95% relative humidity

0.04 to 20% CO2

Workcell Integrated Incubator

Temperature range: 33 to 50°C

Ambient to 95% relative humidity

0.04 to 20% CO2

Workcell Integrated Incubator

Temperature range: 33 to 50°C

Ambient to 95% relative humidity

0.04 to 20% CO2

SCIENTIFIC INSTRUMENTATION

INSTRUMENTS MENU

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