SCIENTIFIC INSTRUMENTATION


SEPARATIONS

ExperimentSolidPhaseExtraction[]

Small Robotic Liquid Handler

  • 20 PSI pressure push (independent for each vessel)
  • 0.1 to 100mL/min flow rates for solvent pushes
  • Up to 20 L of wash solvent per batch
  • Up to 700 mL of equilibration buffer and elution buffer per batch
  • Filter through 3 cc or 6 cc SPE cartridges
  • Collects in SBS deep well plates

ExperimentHPLC[]

Example applications include: Protein purification, Carbohydrate and oligosaccharides analysis, Polynucleotide separation, trace impurity analysis, Liquid chromatography, Affinity chromatography, Ion-exchange chromatography, Size exclusion chromatography, Reverse phase chromatography, Normal phase chromatography, Chiral chromatography

Analytical HPLC with Photo Diode Array and Fluorescence Detector

Quaternary Pump

  • 0.1 to 2.2 mL/min flow rate
  • 7,000 PSI maximum pressure
  • 0.01 inch inner diameter PEEK system tubing
  • Dynamic mixer
  • In-line degasser
  • Titanium pump casing with sapphire pump heads
  • 250 mL to 5 L buffer source bottles/carboys

Detectors

  • Absorbance diode array (PDA)
    • Deuterium lamp
    • 190 to 500 nm absorbance detection (1.2 nm resolution)
    • Quartz flow cell
      • 10 mm pathlength
      • 0.5 µL volume
      • 1,000 PSI maximum pressure

Column Oven

  • 20 to 90°C (± 0.1°C) incubation
  • Up to 2 columns connected in series per run
  • 300 mm maximum column length
  • 15 mm maximum column outer diameter

Sample Constraints

  • 75 µL sample loop
  • 250 µL autosampler syringe
  • Sample chamber temperature controlled from 5 to 40°C (± 3°C)
  • Up to 48x HPLC/CE vials or 2x SBS style deep well plates per batch

Waters Acuity H Class
Waters Acuity FLR

Analytical HPLC with Evaporative Light Scattering Detector

Quaternary Pump

  • 0.1 to 2.2 mL/min flow rate
  • 7,000 PSI maximum pressure
  • 0.01 inch inner diameter PEEK system tubing
  • Dynamic mixer
  • In-line degasser
  • Titanium pump casing with sapphire pump heads
  • 250 mL to 5 L buffer source bottles/carboys

Detectors

  • Ultraviolet absorbance
    • Deuterium lamp
    • 190 to 700 nm wavelength range
      • Fused silica flow cell with 10 mm pathlength
      • 0.5 µL volume
      • 1,000 PSI maximum pressure
    • Up to 80 points/second sampling rate
  • Evaporative Light Scattering (ELS)
    • Nitrogen sheath gas
      • Flow rate between 20 and 60 PSI
      • Heatable to varying magnitude
    • 2 mL/min maximum flow rate
    • Up to 80 points/second sampling rate
    • Drift tube up to 100°C
    • Tungsten halogen light source

Column Oven

  • 20 to 90°C (± 0.1°C) incubation
  • Up to 2 columns connected in series per run
  • 300 mm maximum column length
  • 15 mm maximum column outer diameter

Sample Constraints

  • 75 µL sample loop
  • 250 µL autosampler syringe
  • Sample chamber temperature controlled from 5 to 40°C (± 3°C)
  • Up to 48x HPLC/CE vials or 2x SBS style deep well plates per batch
Waters Acuity H Class
Waters Acuity ELS

Semi-Preparative HPLC with UV/Vis Detector

Quaternary Pump

  • 0.2 to 8 mL/min flow rate
  • 300 PSI minimum pressure
  • 4,000 PSI maximum pressure
  • 0.01 inch inner diameter PEEK system tubing
  • Static mixer
  • In-line degasser
  • Titanium pump casing with sapphire pump heads
  • 250 mL to 20 L buffer source bottles/carboys

Detectors

  • Pump pressure sensor
  • Column temperature sensor
  • Absorbance diode
    • Tungsten deuterium lamp
    • 190 to 900 nm monochromator
    • 6 nm bandpass
    • Quartz flow cell
      • 10 mm pathlength
      • 11 µL volume
      • 725 PSI maximum pressure

Column Oven

  • 5 to 80°C (± 0.1°C) incubation
  • 300 mm maximum column length
  • 25 mm maximum column outer diameter

Fraction Collection

  • Absorbance-based fraction collection methods
    • Absolute threshold
    • Derivative detection
    • Time based
  • Up to 8x 96-well deep well plate fraction containers per batch

Sample Constraints

  • 500 µL sample loop
  • 500 µL autosampler syringe
  • Up to 40x HPLC/CE vials or 2x SBS style deep well plates per batch

Dionex ultimate 3000 with variable wavelength detector

Semi-Preparative HPLC with pH/Conductivity, UV/Vis, and Fluorescence Detectors

  • 0.2 to 8 mL/min flow rate
  • 300 PSI minimum pressure
  • 4,000 PSI maximum pressure
  • 0.01 inch inner diameter PEEK system tubing
  • Static mixer
  • In-line degasser
  • Titanium pump casing with sapphire pump heads
  • 250 mL to 20 L buffer source bottles/carboys

Quaternary Pump

Detectors

  • Pump pressure sensor
  • Column temperature sensor
  • Absorbance diode
    • Tungsten deuterium lamp
    • 190 to 900 nm monochromator
    • 6 nm bandpass
    • Quartz flow cell
      • 10 mm pathlength
      • 11 µL volume
      • 725 PSI maximum pressure
  • Fluorescence
    • 8 µL flow cell volume
    • 290 PSI maximum pressure
    • 200 to 880 nm for excitation light (xenon flash lamp source)
    • 220 to 900 nm for emission light

Column Oven

  • 5 to 80°C (± 0.1°C) incubation
  • 300 mm maximum column length
  • 25 mm maximum column outer diameter

Fraction Collection

  • Absorbance-based fraction collection methods
    • Absolute threshold
    • Derivative detection
    • Time based
  • Up to 8x 96-well deep well plate fraction containers per batch

Sample Constraints

  • 500 µL sample loop
  • 500 µL autosampler syringe
  • Up to 40x HPLC/CE vials or 2x SBS style deep well plates per batch

Dionex Fluorescence detector
Dionex ultimate 3000
Dionex PH/Conductivity Detector

Semi-Preparative HPLC with MALS, DLS, and RI Detector

Quaternary Pump

  • 0.2 to 8 mL/min flow rate
  • 300 PSI minimum pressure
  • 4,000 PSI maximum pressure
  • 0.01 inch inner diameter PEEK system tubing
  • Static mixer
  • In-line degasser
  • Titanium pump casing with sapphire pump heads
  • 250 mL to 20 L buffer source bottles/carboys

Detectors

  • Pump pressure sensor
  • Column temperature sensor
  • Absorbance diode
    • Tungsten deuterium lamp
    • 190 to 900 nm monochromator
    • 6 nm bandpass
    • Quartz flow cell
      • 10 mm pathlength
      • 11 µL volume
      • 725 PSI maximum pressure
  • Multi-angle light scattering
    • Up to 36.6 points/sec sampling rate
    • 200 Da to 1,000 MDa molar mass range
    • 0.5 to 500 nm radius of gyration measurement range
    • Light scattering detectors at 18 angles
    • 658 nm laser wavelength
    • Operating temperature between -15 and 150°C
  • Dynamic light scattering
    • Up to 36.6 points/sec sampling rate
    • 0.5 to 30 nm hydrodynamic radius measurement range
  • Refractive Index detector
    • 7.4 µL flow cell volume
    • Absolute Refractive Index (RI) measurement mode and Differential Refractive Index (DRI) measurement mode available
    • 1.2 to 1.8 RIU Absolute Refractive Index range
    • 0.002 RIU Absolute Refractive Index sensitivity
    • -0.0047 to +0.0047 RIU Differential Refractive Index range
    • ± 7.5 x 10e-10 RIU Differential Refractive Index sensitivity
    • Temperature range 4 and 65°C
    • 660 nm light source
    • Dynamic Range > 16,000,000

Column Oven

  • 5 to 80°C (± 0.1°C) incubation
  • 300 mm maximum column length
  • 25 mm maximum column outer diameter

Fraction Collection

  • Absorbance-based fraction collection methods
    • Absolute threshold
    • Derivative detection
    • Time based
  • Up to 8x 96-well deep well plate fraction containers per batch

Sample Constraints

  • 500 µL sample loop
  • 500 µL autosampler syringe
  • Up to 40x HPLC/CE vials or 2x SBS style deep well plates per batch

Wyatt Dawn w/WYATTQELS Module
Dionex Ultimate 3000
Wyatt Optilab

Semi-Preparative HPLC with MALS, DLS, and RI Detector

Quaternary Pump

  • 0.2 to 8 mL/min flow rate
  • 300 PSI minimum pressure
  • 4,000 PSI maximum pressure
  • 0.01 inch inner diameter PEEK system tubing
  • Static mixer
  • In-line degasser
  • Titanium pump casing with sapphire pump heads
  • 250 mL to 20 L buffer source bottles/carboys

Column Oven

  • 5 to 80°C (± 0.1°C) incubation
  • 300 mm maximum column length
  • 25 mm maximum column outer diameter

Detectors

  • Absorbance diode
    • Tungsten deuterium lamp
    • 190 to 900 nm monochromator
    • 6 nm bandpass
    • Quartz flow cell
      • 10 mm pathlength
      • 11 µL volume
      • 725 PSI maximum pressure
  • Multi-angle light scattering
    • Up to 36.6 points/sec sampling rate
    • 200 Da to 1,000 MDa molar mass range
    • 0.5 to 500 nm radius of gyration measurement range
    • Light scattering detectors at 18 angles
    • 658 nm laser wavelength
    • Operating temperature between -15 and 150°C
  • Refractive Index detector
    • 7.4 µL flow cell volume
    • Absolute Refractive Index (RI) measurement mode and Differential Refractive Index (DRI) measurement mode available
    • 1.2 to 1.8 RIU Absolute Refractive Index range
    • 0.002 RIU Absolute Refractive Index sensitivity
    • -0.0047 to +0.0047 RIU Differential Refractive Index range
    • ± 7.5 x 10e-10 RIU Differential Refractive Index sensitivity
    • Temperature range 4 and 65°C
    • 660 nm light source
    • Dynamic Range > 16,000,000
  • Different viscometer detector
    • Minimum detectable sample at 0.1 µg of 100 kDa polystyrene in THF at 1 mL/min
    • 6.9 kPa differential pressure range
    • 13,500:1 dynamic range
    • Up to 1.5 mL/min for aqueous sample and 3 mL/min for THF
    • 4 ºC to 70 ºC temperature control

Fraction Collection

  • Absorbance-based fraction collection methods
    • Absolute threshold
    • Derivative detection
    • Time based
  • Up to 8x 96-well deep well plate fraction containers per batch

Sample Constraints

  • 500 µL sample loop
  • 500 µL autosampler syringe
  • Up to 40x HPLC/CE vials or 2x SBS style deep well plates per batch
Wyatt Vicostar

Large Scale Preparative HPLC with UV/Vis Detector

Binary Pump

  • Up to 50 mL/min flow rate
  • 6,000 PSI maximum pressure
  • In-line degasser
  • 2 to 20 L buffer source bottles/carboys

Column Selector

  • Ambient temperature
  • Switchable for up to 6 columns

Detectors

  • Pump pressure sensor
  • Column temperature sensor
  • Ultraviolet absorbance detector
  • Deuterium lamp
  • 190 to 600 nm wavelength range (up to two channels)
  • 1 nm wavelength bandpass
  • Up to 120 samples/second measurement (25 samples/second for dual channel) – Quartz flow cell 3 mm pathlength – 4 µL volume – 1,760 PSI maximum pressure

Fraction Collection

  • Absorbance-based fraction collection methods
    • Absolute threshold
    • Derivative detection
    • Time based
  • Up to 8x 96-well deep well plate fraction containers per batch

Sample Constraints

  • 500 µL sample loop
  • 500 µL autosampler syringe
  • Up to 40x HPLC/CE vials or 2x SBS style deep well plates per batch
1290 Infinity 2 Autoscale Preperative LC System

Analytical HPLC with Refractive Index Detector

Semi-Preparative HPLC with UV/Vis Diode Array + Access for 3rd party detectors

Semi-Preparative HPLC with UV/Vis Diode Array + Fluorescence Detectors

Semi-Preparative HPLC with UV/Vis Diode Array Detector

Binary Pump

  • Up to 50 mL/min flow rate
  • 6,000 PSI maximum pressure
  • In-line degasser
  • 2 to 20 L buffer source bottles/carboys

Column Selector

  • Ambient temperature
  • Switchable for up to 6 columns

Detectors

  • Pump pressure sensor
  • Column temperature sensor
  • Ultraviolet absorbance detector
  • Deuterium lamp
  • 190 to 600 nm wavelength range (up to two channels)
  • 1 nm wavelength bandpass
  • Up to 120 samples/second measurement (25 samples/second for dual channel) – Quartz flow cell 3 mm pathlength – 4 µL volume – 1,760 PSI maximum pressure

Fraction Collection

  • Absorbance-based fraction collection methods
    • Absolute threshold
    • Derivative detection
    • Time based
  • Up to 8x 96-well deep well plate fraction containers per batch

Sample Constraints

  • 500 µL sample loop
  • 500 µL autosampler syringe
  • Up to 40x HPLC/CE vials or 2x SBS style deep well plates per batch
Agilent – 1260 Infinity II

Analytical HPLC with Refractive Index Detector

Binary Pump

  • Up to 50 mL/min flow rate
  • 6,000 PSI maximum pressure
  • In-line degasser
  • 2 to 20 L buffer source bottles/carboys

Column Selector

  • Ambient temperature
  • Switchable for up to 6 columns

Detectors

  • Pump pressure sensor
  • Column temperature sensor
  • Ultraviolet absorbance detector
  • Deuterium lamp
  • 190 to 600 nm wavelength range (up to two channels)
  • 1 nm wavelength bandpass
  • Up to 120 samples/second measurement (25 samples/second for dual channel) – Quartz flow cell 3 mm pathlength – 4 µL volume – 1,760 PSI maximum pressure

Fraction Collection

  • Absorbance-based fraction collection methods
    • Absolute threshold
    • Derivative detection
    • Time based
  • Up to 8x 96-well deep well plate fraction containers per batch

Sample Constraints

  • 500 µL sample loop
  • 500 µL autosampler syringe
  • Up to 40x HPLC/CE vials or 2x SBS style deep well plates per batch
Agilent – 1290 II

Semi-Preparative HPLC Viscometer

Differential Viscosity  

  • Sensitivity (minimum detectable sample):  0.1 μg of 100 kDa polystyrene in THF at 1 mL/min, under typical GPC conditions
  • Differential pressure noise: < 0.05 Pa
  • Differential pressure range: 6.9 kPa, protected against overpressure
  • Dynamic range: 135,000:1
  • Linearity:  0.5% of full scale
  • Differential pressure drift: < 2.5 Pa/hr
  • Capillary bridge autobalance:  Thermal, leak-free mechanism
  • Sample shear rate: 5000 Hz with water at 1 mL/min

Fluidics           

  • Max. flow rate: Aqueous: 1.5 mL/min; THF: 3 mL/min
  • Delay volume options: 16.2, 10.8 or 5.4 mL, with additional factory-installed option for 32.4 mL 1
  • Max. permissible pressure inlet to outlet: 690 kPa
  • Wetted Materials:  316 stainless steel, PTFE, zirconium silicate, Kalrez ® , Inconel, 26-1 Stainless Steel

Temperature Control 

  • Range:  4 ºC to 70 ºC
  • Stability:  <0.005°C
  • Ambient Range:  0 ºC to 40 ºC, 20% to 70% RH

Electronics     

  • Digital Output 20 bits @ 10 Hz
  • Auxiliary I/O   
    • Inputs:  Autoinject contact closure, Alarm In, Analog In: 2 differential inputs, 18 bits resolution, range -10 V to +10 V
    • Outputs: Autoinject Retransmit, Alarm Out, Recycle Out
  • On-board Computer  
    • Communications         Ethernet
  • Front Panel Display: 7″ (178 mm), high-resolution, multi-touch display for viewing/setting signals, instrument settings and diagnostics.
  • Dimensions:  58 cm (L) x 36 cm (W) x 18 cm (H)
Wyatt – Vicostar

ExperimentGasChromatography[]

Gas Chromatography System

Flow System

  • He carrier gas available
  • Inlets available:
    • Split/Splitless capillary column inlet
    • Multimode temperature-programmable capillary column inlet
    • Column head pressure up to 100 PSIG

Column Oven

  • Temperature range: 30 to 450ºC
  • Up to 120ºC/min temperature ramp
  • Up to 2 capillary columns
  • 0.53 mm maximum column inner diameter

Split/Splitless Capillary Inlet

  • Suitable for capillary columns up to 0.53 mm inner diameter
  • Split ratio up to 7,500:1 to prevent column overload
  • Up to 1,250 mL/min total flow of He
  • Inlet temperature 30 to 400ºC

Multimode Capillary Inlet

  • Suitable for capillary columns up to 0.53 mm inner diameter
  • Split ratio up to 7,500:1 to prevent column overload
  • Up to 1,250 mL/min total flow of He
  • Inlet temperature 30 to 400ºC
  • Temperature programmable with ramp rates up to 900ºC/min

Mass Spectrometry

  • Single quadrupole mass spectrometer
  • 1.6 to 1,050 m/z mass range
  • 100 to 200ºC quadrupole temperature
  • 100 to 300ºC ion source temperature
  • 100 to 350ºC transfer line temperature
  • Scan speeds up to 12,500 m/z per second
  • Electron ionization:
    • Dual filaments
  • Chemical ionization:
    • Single filament
    • Methane reagent gas
    • Positive and negative mode

Flame Ionization Detector

  • Responds to most organic compounds
  • Minimum detectable level (for tridecane): < 1.2 pgC/s
  • Linear dynamic range: > 10⁷ (± 10%)
  • Up to 1,000 points/second data measurement

Autosampler Rail System

  • Vortex mixing up to 2,000 RPM
  • Sample incubation 30 to 200ºC
  • Liquid, headspace, and solid-phase microextraction sample preparation available
  • 0.01 to 100 µL injection volumes for liquid injection
  • 25 to 2,500 µL injection volumes for headspace injection
  • Headspace syringe heating 40 to 150ºC

Agilent 8890 CS System

ExperimentIonChromatography[]

Ion Chromatography System

Pumps

  • Dual channel system
    • Anion Channel: Isocratic pump with pure water being primary solvent
    • Cation Channel: Quaternary pump
  • 0.1 to 10 mL/min flow rate
  • 5,000 PSI maximum pressure
  • Static mixer
  • In-line degasser

Eluent Generator

  • Electrolytic eluent generation for anionic applications
  • 0.1 to 100 mM concentration

Dynamically Regenerated Suppressors

  • Constant voltage and constant current mode
  • Maximum suppressor capabilities:
    • Anion channel: 50 μeq/min
    • Cation channel: 35 μeq/min
  • 0.25 to 0.75 mL/min flow rate

Conductance Detectors

  • Passivated 316 stainless steel electrode
  • Maximum range up to 15,000 μS conductance measurement
  • 0.7 µL flow cell volume
  • One detector for each channel
  • Shared detection oven with 15 to 60ºC (± 0.5ºC) incubation

Column Oven

  • 10 to 70°C (± 0.1°C) incubation
  • One analytical column for each channel

Sample Constraints

  • 10 µL sample loops
  • Up to 40x HPLC/CE vials or 2x SBS style deep well plates per batch
  • Sample chamber temperature controlled from 4 to 60°C

Dionex ICS-6000 HPIC System

Ion Chromatography System with Electrochemical Detector

Quaternary Pump

  • Single channel system
  • 0.1 to 10 mL/min flow rate
  • 5,000 PSI maximum pressure
  • Static mixer
  • In-line degasser

Ultraviolet absorbance detector

  • Tungsten and Deuterium lamp
  • 190 to 900 nm wavelength range (up to four channels)
  • 6 nm wavelength bandpass
  • Up to 100 samples/second measurement
  • PEEK flow cell
    • 10 mm path length
    • 11 μL flow cell volume

Electrochemical detector

  • < 0.2 μL internal volume
  • 100 PSI maximum pressure
  • Gold, platinum, carbon and silver working electrodes
  • pH-Ag/AgCl reference electrode

Column Oven

  • 10 to 70°C (± 0.1°C) incubation

Sample Constraints

  • 25 μL sample loop
  • Up to 40x HPLC/CE vials or 2x SBS style deep well plates per batch
  • Sample chamber temperature controlled from 4 to 60°C

Dionex ICS-6000 HPIC System
Dionex eletrochemical detector

ExperimentFPLC[]

Example applications include: Aqueous mobile phase, Protein purification, Biomolecule purification, Fast protein liquid chromatography, Medium-pressure chromatography, Liquid chromatography, Affinity chromatography

Fast Protein Liquid Chromatography System Medium and High Flow

System Pump & Mixer

  • 0.001 to 25 mL/min flow rate (50 mL/min for column packing) for Avant 25
  • 0.001 to 150 mL/min flow rate (300 mL/min for column packing) for Avant 150
  • 20 MPa maximum pressure for Avant 25
  • 5 MPa maximum pressure for Avant 150
  • 1.4 L mixer volume
  • ± 0.6% gradient composition accuracy for Avant 25
  • ± 0.8% gradient composition accuracy for Avant 150

Detectors

  • Pump pressure sensor
    • 0 to 20 MPa range
    • ± 0.02 MPa accuracy
  • Temperature monitor
    • ± 1.5°C temperature accuracy
  • Conductance sensor
    • 0.01 mS/cm to 1 S/cm conductance range
    • 22 µL flow cell volume
  • Absorbance monitor
    • 190 to 700 nm wavelength range in increments of 1 nm wavelength
    • Up to 3 simultaneous wavelengths
    • UV flow cell
      • 0.5 mm, 2 mm, or 10 mm pathlengths
      • 1 μL, 2 μL, or 8 μL volumes
      • Noise < 0.08 mAU
  • pH monitor
    • 0 to 14 range
    • ± 0.1 pH unit (within pH 2 to 12)

Fraction Collection

  • Absorbance-based or conductivity-based fraction collection methods
    • Absolute threshold
    • Derivative detection
    • Time based
    • Start collection with sample application or within the gradient
  • 50 mL container, 15 mL container, or 96-well deep well plate fraction collection

Sample Constraints

  • Autosampler mode
    • 50 to 10,000 µL sample loop volume
    • 1,000 to 10,000 µL autosampler syringe
    • Up to 168x HPLC/CE vials or 2x SBS style deep well plate per batch
    • Up to 12 large sample vials (10 mL) for preparative system
  • Flow injection mode
    • Up to 50 mL to 4 L sample container volumes
    • Up to 5 containers
    • Controllable sample introduction rate (up to 150 mL/min)
  • Superloop mode
    • 10 mL loop volume
    • Up to 50 mL to 4 L sample container volumes
    • Up to 5 containers

AKTA AVANT 25 and 150

ExperimentFlashChromatography[]

Flash Chromatography System

Binary Pump

  • 500 to 200 mL/min flow rate
  • 200 PSI maximum pressure
  • Up to 20 L drum solvent sources
  • Programmable gradient (± 1% accuracy)

Absorbance Detector

  • Deuterium lamp
  • 200 nm and 780 nm UV detection (5 nm bandpass)
  • 0.6 µl flow cell (0.1 mm pathlength)

Fraction Collection

  • 30x 50 mL vials per batch
  • 70x 25 mL vials per batch

Column Constraints

  • Luer lock connector pre-packed columns
  • Up to 70 mm outer diamter flash columns
  • Up to 16.5″ length flash columns
  • ~5 to ~660 g of resin per column

Sample Constraints

  • Up to 33 g of solid material for dry loading
  • Up to 50 mL of liquid material for wet loading

Teledyne combiflash RF 200

ExperimentCapillaryGelElectrophoresisSDS[]

Protein Capillary Electrophoresis System

  • 10 to 270 KDa molecular weight range
  • Programmable sample electrokinetic injection and separation
    • Up to 20 steps
    • 0 to 6,500 V
  • Sequential analysis on a single capillary
  • Samples can be held at 4ºC, 10ºC, 15ºC, or ambient temperature while enqueued for injection

Detection

  • UV absorbance: 220 nm
  • Sensitivity: 0.3 µg/mL

Sample Constraints

  • Minimum sample volume: 50 µL
  • Up to 48 injections per batch
  • Up to 12 injections per sample
  • Up to 100 samples per cartridge
  • Protein concentration: 0.2 to 2 mg/mL
  • Salt concentration: up to 50 mM

ProteinSimple Maurice

ExperimentCapillaryIsoelectricFocusing[]

Protein Capillary Electrophoresis System

  • 2.85 to 10.45 isoelectric point range
  • Programmable focusing
    • Up to 20 steps
    • 0 to 5,000 V
  • Modular ampholyte and internal standard composition
  • Optional onboard mixing for sensitive samples
  • Sequential analysis on a single capillary
  • Samples can be held at 4ºC, 10ºC, 15ºC, or ambient temperature while enqueued for injection

Detection

  • Whole capillary imaging using a CCD
  • Sensitivity
    • UV absorbance: 3 µg/mL
    • Native fluorescence: 0.7 µg/mL
  • Detectors
    • UV absorbance: 280 nm
    • Native fluorescence:
      • Excitation: 280 nm
      • Emission: 320 to 405 nm bandpass

Sample Constraints

  • Minimum sample volume: 50 µL
  • Up to 100 injections per batch
  • Up to 12 injections per sample
  • Up to 100 samples per cartridge
  • Protein concentration: ~0.2 mg/mL
  • Salt concentrations: up to 15 mM

ProteinSimple Maurice

ExperimentPAGE[]

ExperimentAgaroseGelElectrophoresis[]

Robotic Gel Electrophoresis System

Polyacrylamide Gel Electrophoresis

  • Slab Gels
    • 4.5% and 10% TBE-Urea gels
    • 10% TBE gels
    • 7% and 12% Tris-Glycine gels
    • 5 mm width lanes
    • 3 mm depth gels
  • Electrophoresis
    • 0 to 125 V
    • 1 to 100% duty cycle options
  • Fluorescence Imaging
    • Post stained gels (stained with any custom solution)
    • Excitation filters
      • 470 nm (70 nm bandpass)
      • 625 nm (70 nm bandpass)
    • Emission filters
      • 540 nm (40 nm bandpass)
      • 678 nm (44 nm bandpass)
  • Sample Constraints
    • Up to 20 µL of sample and loading buffer per lane
    • Up to 10 lanes per gel
    • Up to 4 gels per batch

Agarose Gel Electrophoresis

  • Gels
    • Analytics (no sample recovery) or size selection (targeted recovery of analytes of particular sizes)
    • 0.5, 1.0, 1.5, 2.0, and 3.0% agarose
    • Independent lanes
  • Electrophoresis
    • 100 V
    • 1 to 100% duty cycle options
  • Fluorescence Imaging
    • Pre-stained markers ranging from 25 to 20,000 base pairs
    • Multiple in-run images
    • Excitation filters
      • 470 nm (70 nm bandpass)
      • 625 nm (70 nm bandpass)
    • Emission filters
      • 540 nm (40 nm bandpass)
      • 678 nm (44 nm bandpass)
  • Sample Constraints (Size Selection)
    • Up to 50 µL of sample and loading buffer per lane
    • Up to 12 lanes per gel
    • Up to 4 gels per batch
  • Sample Constraints (Analytical)
    • Up to 8 µL of sample and loading buffer per lane
    • Up to 23 lanes per gel
    • Up to 4 gels per batch


ExperimentWestern[]

ExperimentTotalProteinDetection[]

Example applications include: Capillary-based Analytical Assay, Gel Electrophoresis, Capillary Gel Electrophoresis, Total Protein Separation, Total Protein Visualization, Protein Size Characterization, Protein Abundance Comparison

Capillary Western Blot System

Capillary Gel Electrophoresis

  • 2 to 440 kDa molecular weight range
  • 0 to 500 V separation voltage
  • UV capillary crosslinking for protein immobilization

Secondary Antibodies Available

  • Secondary Antibodies Available
    • Goat Anti-Rabbit HRP
    • Goat Anti-Mouse HRP
    • Goat Anti-Human IgG HRP
    • Donkey Anti-Goat HRP

CCD Camera-Based Capillary Imaging

  • 100 ms to 512 sec exposure times
  • Up to picogram sensitivity (depending on the primary antibody sensitivity)

Capacity

  • 13 or 25 capillaries per batch
  • 5 to 8 µL of sample required per capillary
ProteinSimple Jess

ExperimentCrossFlowFiltration[]

Example applications include: Ultrafiltration, Microfiltration, Diafiltration, Protein Purification, Tangential Flow Filtration, Buffer Exchange, Desalting

Tangential Flow Filtration System

  • Volume range of 10 mL to 2 L
  • Filtration area of 13 to 3,700 cm²
  • Digitally controlled peristaltic pump
  • Digital pressure monitor
  • Automatic backpressure valve
  • Digital conductivity monitors
  • Absorbance monitor of 200 to 800 nm
  • UV-Vis spectrometer
    • 200 to 1,100 nm wavelength range
    • CCD detector allows the entire wavelength
    • Sub-nanometer resolution
    • Up to 200 measurements/sec

µPULSE TFF system
Ocean Insight Flame UV-VIS Spectrometer

ExperimentFieldFlowFractionation[]

Example applications include: Ultrafiltration, Microfiltration, Diafiltration, Protein Purification, Tangential Flow Filtration, Buffer Exchange, Desalting

Multiflow FFF System

Isocratic Pump

  • Flow rate: 0.05 to 4 mL/min (micro), 0.05 to 9.95 mL-min (analytical), 0.2 to 40 mL/min (preparative)
  • Maximum pressure: 40 MPa (micro), 40 MPa (analytical), 20 MPa (preparative)

Detectors

  • Refractory Index detector: 8 µL flow cell volume
  • UV/Vis detector: 4-channel diode array detector, wavelength 190-800nm, 10µL flow cell volume, 10 mm path length
    • Goat Anti-Rabbit HRP
  • 21-angle Multi-angle Laser Light Scattering detector: using a 532nm laser at 50mW

Cartridges

  • Modular spacers and membranes on FFF cartridges for various applicaitons: asymmetric flow, electrical asymmetric flow, and size exclusion, as well as disposable hollow-fiber cartridges

Autosampler

  • Injection modes: full-loop, partial loop, and ul pickup
  • Injection volume: 1 to 1,000 µL (up to 10 mL for preparative applications)
  • Temperature control: 4 to 40ºC
  • Sample shaking

Fraction collector

  • Analytical and preparative (up to 10mg) scale applications
  • Collection mode: targeted peaks and peak fractions
  • Temperature control – 5-40Celsius
  • Collection with needle directly to 2 ml vials, 96 well plates, or 10 ml preparative vials

Sample Constraints

  • Particle size: up to 1Micrometer (Brownian mode), over 1Micrometer (Steric mode)
  • Ideal for characterization of proteins, aggregates, liposomes, nanoparticles and various macromolecules
Postnova AF2000 Multiflow FFF System

ExperimentDialysis[]

Example applications include: Fragile Proteins, Viscous Fluids, Polymer Gels, Equilibrium Dialysis, Small Molecule Binding to Proteins, Purification, Buffer Exchange, Desalting, Protein Purification

Static Dialysis

Beakers Available

  • 250 mL, 600 mL, 1 L, and 2 L
  • Incubation range 5 to 45°C (below ambient temperatures available for 250 mL beakers only)
  • Buffer mixing available

Tube-Based Dialysis Kits

  • 3.5k, 6 to 8k and 12 to 14k molecular weight cutoffs (MWCOs) available
  • 10 µL to 20 mL sample volume range
  • 4 to 60°C incubation range
  • Up to 9 samples per batch

Dialysis Tubing

  • 3.5k, 6 to 8k, and 12 to 14k MWCOs available
  • 30 to 130 mL sample volume range
  • Continuous buffer flow to increase separation efficiency
  • 1 sample per batch

Equilibrium Dialysis

  • 8 MWCO available
  • 50 to 500 µL sample volume range
  • 4 to 60°C incubation range
  • Up to 48 samples per batch
Tube based dialysis kit
Dialysis Tubing
Equilibrium Dialysis

Dynamic Dialysis

  • 3.5k, 6k, and 12k MWCOs available
  • 30 to 130 mL sample volume range
  • Continuous buffer flow to increase separation efficiency
  • > 90% protein recovery after dialysis
  • 1 sample per batch
Spectraflo Lab System

ExperimentThinLayerChromatography[]

High Performance TLC System (HPTLC)

Derivatization

  • Automated micro-droplet spraying of chemical stains
  • Application of aqueous or organic solutions
  • Spot-to-spot relative standard deviation <5%

Application

  • Up to 15 samples per HPTLC plate (20 x 10 cm)
  • 5 to 6µm silica particle size
  • Samples applied to plates as 8 mm bands for HPTLC
  • Samples applied as spots for reaction monitoring

Development

  • Gas phase can be actively controlled to ensure saturation of elution chamber
  • Solvent front monitoring and automatic plate removal
  • Development of TLC and HPTLC plate up to 20×20 cm

Visualization

  • UV (254 and 366 nm) and white light illumination
  • Visualize TLC and HPTLC plates up to 20×20 cm
  • Rf evaluation and comparison within or between plates
  • Semi-quantitative peak integration
Camag Derivatizer
Cama Automatic TLC Sampler 4 (ATS 4)
Camag Automatec Developing Chamber 2
Camag TLC Visualizer 2

ExperimentLiquidExtraction[]

Phase Separator

  • Rapidly separates immiscible mixtures of organic and aqueous solutions via filtration
  • Separation does not depend on whether the aqueous layer is on the top or bottom
  • Up to 70 ML capacity per filtration
  • Organic solvent flow rate up to 15 mL/min

Separatory Funnel

  • Collects aqueous and organic layers of an immiscible mixture at a volume range of 10 mL to 1 L

Robotic Extraction

  • Separate top layer from bottom layer given exact height of the top and bottom layer
  • Control aspiration flow rate
Phase Separator
Separatory Funnel
Gilson GX-271
Hamilton Star

ExperimentMagneticBeadSeparation[]

Example applications include: Magnetic Cell Separation, Protein Purification, Immunoprecipitation, Nucleic Acid Isolation

Magnetic Racks

Robotic Separation

  • Performed by a Hamilton liquid handler with the Alpaqua Magnum FLX magnet
  • Sample volume constraints
    • 96-well plate magnet: up to 2 mL

Manual Separation

  • Performed by an operator with the DynaMag magnets
  • Sample volume constraints
    • 96-well plate magnet: up to 0.2 mL
    • 2 mL tube magnet: up to 2 mL
    • 15 mL tube magnet: up to 15 mL
    • 50 mL tube magnet: up to 50 mL