SCIENTIFIC INSTRUMENTATION

Small Robotic Liquid Handler

 

• 20 PSI pressure push (independent for each vessel)

• 0.1 to 100mL/min flow rates for solvent pushes

• Up to 20 L of wash solvent per batch

• Up to 700 mL of equilibration buffer and elution buffer per batch

• Filter through 3 cc or 6 cc SPE cartridges

• Collects in SBS deep well plates

Analytical HPLC with Photo Diode Array and Fluorescence Detector

Quaternary Pump

 

• 0.1 to 2.2 mL/min flow rate

• 7,000 PSI maximum pressure

• 0.01 inch inner diameter PEEK system tubing

• Dynamic mixer

• In-line degasser

• Titanium pump casing with sapphire pump heads

• 250 mL to 5 L buffer source bottles/carboys

Detectors

 

• Absorbance diode array (PDA)

   

  • Deuterium lamp
  • 190 to 500 nm absorbance detection (1.2 nm resolution)
  • Quartz flow cell

     

    • 10 mm pathlength
    • 0.5 µL volume
    • 1,000 PSI maximum pressure

Column Oven

 

• 20 to 90°C (± 0.1°C) incubation

• Up to 2 columns connected in series per run

• 300 mm maximum column length

• 15 mm maximum column outer diameter

Sample Constraints

 

• 75 µL sample loop

• 250 µL autosampler syringe

• Sample chamber temperature controlled from 5 to 40°C (± 3°C)

• Up to 48x HPLC/CE vials or 2x SBS style deep well plates per batch

Analytical HPLC with Evaporative Light Scattering Detector

Quaternary Pump

 

• 0.1 to 2.2 mL/min flow rate

• 7,000 PSI maximum pressure

• 0.01 inch inner diameter PEEK system tubing

• Dynamic mixer

• In-line degasser

• Titanium pump casing with sapphire pump heads

• 250 mL to 5 L buffer source bottles/carboys

Detectors

 

• Ultraviolet absorbance

   

  • Deuterium lamp
  • 190 to 700 nm wavelength range

     

    • Fused silica flow cell with 10 mm pathlength
    • 0.5 µL volume
    • 1,000 PSI maximum pressure

  • Up to 80 points/second sampling rate

• Evaporative Light Scattering (ELS)

   

  • Nitrogen sheath gas

     

    • Flow rate between 20 and 60 PSI
    • Heatable to varying magnitude

  • 2 mL/min maximum flow rate
  • Up to 80 points/second sampling rate
  • Drift tube up to 100°C
  • Tungsten halogen light source

Column Oven

 

• 20 to 90°C (± 0.1°C) incubation

• Up to 2 columns connected in series per run

• 300 mm maximum column length

• 15 mm maximum column outer diameter

Sample Constraints

 

• 75 µL sample loop

• 250 µL autosampler syringe

• Sample chamber temperature controlled from 5 to 40°C (± 3°C)

• Up to 48x HPLC/CE vials or 2x SBS style deep well plates per batch

Semi-Preparative HPLC with UV/Vis Detector

Quaternary Pump

 

• 0.2 to 8 mL/min flow rate

• 300 PSI minimum pressure

• 4,000 PSI maximum pressure

• 0.01 inch inner diameter PEEK system tubing

• Static mixer

• In-line degasser

• Titanium pump casing with sapphire pump heads

• 250 mL to 20 L buffer source bottles/carboys

Detectors

 

• Pump pressure sensor

• Column temperature sensor

• Absorbance diode

   

  • Tungsten deuterium lamp
  • 190 to 900 nm monochromator
  • 6 nm bandpass
  • Quartz flow cell

     

    • 10 mm pathlength
    • 11 µL volume
    • 725 PSI maximum pressure

Column Oven

 

• 5 to 80°C (± 0.1°C) incubation

• 300 mm maximum column length

• 25 mm maximum column outer diameter

Fraction Collection

 

• Absorbance-based fraction collection methods

   

  • Absolute threshold
  • Derivative detection
  • Time based

• Up to 8x 96-well deep well plate fraction containers per batch

Sample Constraints

 

• 500 µL sample loop

• 500 µL autosampler syringe

• Up to 40x HPLC/CE vials or 2x SBS style deep well plates per batch

Semi-Preparative HPLC with pH/Conductivity, UV/Vis, and Fluorescence Detectors

 

• 0.2 to 8 mL/min flow rate

• 300 PSI minimum pressure

• 4,000 PSI maximum pressure

• 0.01 inch inner diameter PEEK system tubing

• Static mixer

• In-line degasser

• Titanium pump casing with sapphire pump heads

• 250 mL to 20 L buffer source bottles/carboys

Quaternary Pump

Detectors

 

• Pump pressure sensor

• Column temperature sensor

• Absorbance diode

   

  • Tungsten deuterium lamp
  • 190 to 900 nm monochromator
  • 6 nm bandpass
  • Quartz flow cell

     

    • 10 mm pathlength
    • 11 µL volume
    • 725 PSI maximum pressure

• Fluorescence

   

  • 8 µL flow cell volume
  • 290 PSI maximum pressure
  • 200 to 880 nm for excitation light (xenon flash lamp source)
  • 220 to 900 nm for emission light

Column Oven

 

• 5 to 80°C (± 0.1°C) incubation

• 300 mm maximum column length

• 25 mm maximum column outer diameter

Fraction Collection

 

• Absorbance-based fraction collection methods

   

  • Absolute threshold
  • Derivative detection
  • Time based

• Up to 8x 96-well deep well plate fraction containers per batch

Sample Constraints

 

• 500 µL sample loop

• 500 µL autosampler syringe

• Up to 40x HPLC/CE vials or 2x SBS style deep well plates per batch

Semi-Preparative HPLC with MALS, DLS, and RI Detector

Quaternary Pump

 

• 0.2 to 8 mL/min flow rate

• 300 PSI minimum pressure

• 4,000 PSI maximum pressure

• 0.01 inch inner diameter PEEK system tubing

• Static mixer

• In-line degasser

• Titanium pump casing with sapphire pump heads

• 250 mL to 20 L buffer source bottles/carboys

Detectors

 

• Pump pressure sensor

• Column temperature sensor

• Absorbance diode

   

  • Tungsten deuterium lamp
  • 190 to 900 nm monochromator
  • 6 nm bandpass
  • Quartz flow cell

     

    • 10 mm pathlength
    • 11 µL volume
    • 725 PSI maximum pressure

• Multi-angle light scattering

   

  • Up to 36.6 points/sec sampling rate
  • 200 Da to 1,000 MDa molar mass range
  • 0.5 to 500 nm radius of gyration measurement range
  • Light scattering detectors at 18 angles
  • 658 nm laser wavelength
  • Operating temperature between -15 and 150°C

• Dynamic light scattering

   

  • Up to 36.6 points/sec sampling rate
  • 0.5 to 30 nm hydrodynamic radius measurement range

• Refractive Index detector

   

  • 7.4 µL flow cell volume
  • Absolute Refractive Index (RI) measurement mode and Differential Refractive Index (DRI) measurement mode available
  • 1.2 to 1.8 RIU Absolute Refractive Index range
  • 0.002 RIU Absolute Refractive Index sensitivity
  • -0.0047 to +0.0047 RIU Differential Refractive Index range
  • ± 7.5 x 10e-10 RIU Differential Refractive Index sensitivity
  • Temperature range 4 and 65°C
  • 660 nm light source
  • Dynamic Range > 16,000,000

Column Oven

 

• 5 to 80°C (± 0.1°C) incubation

• 300 mm maximum column length

• 25 mm maximum column outer diameter

Fraction Collection

 

• Absorbance-based fraction collection methods

   

  • Absolute threshold
  • Derivative detection
  • Time based

• Up to 8x 96-well deep well plate fraction containers per batch

Sample Constraints

 

• 500 µL sample loop

• 500 µL autosampler syringe

• Up to 40x HPLC/CE vials or 2x SBS style deep well plates per batch

Semi-Preparative HPLC with MALS, DLS, and RI Detector

Quaternary Pump

 

• 0.2 to 8 mL/min flow rate

• 300 PSI minimum pressure

• 4,000 PSI maximum pressure

• 0.01 inch inner diameter PEEK system tubing

• Static mixer

• In-line degasser

• Titanium pump casing with sapphire pump heads

• 250 mL to 20 L buffer source bottles/carboys

Column Oven

 

• 5 to 80°C (± 0.1°C) incubation

• 300 mm maximum column length

• 25 mm maximum column outer diameter

Detectors

 

• Absorbance diode

   

  • Tungsten deuterium lamp
  • 190 to 900 nm monochromator
  • 6 nm bandpass
  • Quartz flow cell

     

    • 10 mm pathlength
    • 11 µL volume
    • 725 PSI maximum pressure

• Multi-angle light scattering

   

  • Up to 36.6 points/sec sampling rate
  • 200 Da to 1,000 MDa molar mass range
  • 0.5 to 500 nm radius of gyration measurement range
  • Light scattering detectors at 18 angles
  • 658 nm laser wavelength
  • Operating temperature between -15 and 150°C

• Refractive Index detector

   

  • 7.4 µL flow cell volume
  • Absolute Refractive Index (RI) measurement mode and Differential Refractive Index (DRI) measurement mode available
  • 1.2 to 1.8 RIU Absolute Refractive Index range
  • 0.002 RIU Absolute Refractive Index sensitivity
  • -0.0047 to +0.0047 RIU Differential Refractive Index range
  • ± 7.5 x 10e-10 RIU Differential Refractive Index sensitivity
  • Temperature range 4 and 65°C
  • 660 nm light source
  • Dynamic Range > 16,000,000

• Different viscometer detector

   

  • Minimum detectable sample at 0.1 µg of 100 kDa polystyrene in THF at 1 mL/min
  • 6.9 kPa differential pressure range
  • 13,500:1 dynamic range
  • Up to 1.5 mL/min for aqueous sample and 3 mL/min for THF
  • 4 ºC to 70 ºC temperature control

Fraction Collection

 

• Absorbance-based fraction collection methods

   

  • Absolute threshold
  • Derivative detection
  • Time based

• Up to 8x 96-well deep well plate fraction containers per batch

Sample Constraints

 

• 500 µL sample loop

• 500 µL autosampler syringe

• Up to 40x HPLC/CE vials or 2x SBS style deep well plates per batch

Large Scale Preparative HPLC with UV/Vis Detector

Binary Pump

 

• Up to 50 mL/min flow rate

• 6,000 PSI maximum pressure

• In-line degasser

• 2 to 20 L buffer source bottles/carboys

Column Selector

 

• Ambient temperature

• Switchable for up to 6 columns

Detectors

 

• Pump pressure sensor

• Column temperature sensor

• Ultraviolet absorbance detector

• Deuterium lamp

• 190 to 600 nm wavelength range (up to two channels)

• 1 nm wavelength bandpass

• Up to 120 samples/second measurement (25 samples/second for dual channel) – Quartz flow cell 3 mm pathlength – 4 µL volume – 1,760 PSI maximum pressure

Fraction Collection

 

• Absorbance-based fraction collection methods

   

  • Absolute threshold
  • Derivative detection
  • Time based

• Up to 8x 96-well deep well plate fraction containers per batch

Sample Constraints

 

• 500 µL sample loop

• 500 µL autosampler syringe

• Up to 40x HPLC/CE vials or 2x SBS style deep well plates per batch

Analytical HPLC with Refractive Index Detector

Semi-Preparative HPLC with UV/Vis Diode Array + Access for 3rd party detectors

Semi-Preparative HPLC with UV/Vis Diode Array + Fluorescence Detectors

Semi-Preparative HPLC with UV/Vis Diode Array Detector

Binary Pump

 

• Up to 50 mL/min flow rate

• 6,000 PSI maximum pressure

• In-line degasser

• 2 to 20 L buffer source bottles/carboys

Column Selector

 

• Ambient temperature

• Switchable for up to 6 columns

Detectors

 

• Pump pressure sensor

• Column temperature sensor

• Ultraviolet absorbance detector

• Deuterium lamp

• 190 to 600 nm wavelength range (up to two channels)

• 1 nm wavelength bandpass

• Up to 120 samples/second measurement (25 samples/second for dual channel) – Quartz flow cell 3 mm pathlength – 4 µL volume – 1,760 PSI maximum pressure

Fraction Collection

 

• Absorbance-based fraction collection methods

   

  • Absolute threshold
  • Derivative detection
  • Time based

• Up to 8x 96-well deep well plate fraction containers per batch

Sample Constraints

 

• 500 µL sample loop

• 500 µL autosampler syringe

• Up to 40x HPLC/CE vials or 2x SBS style deep well plates per batch

 

Analytical HPLC with Refractive Index Detector

Binary Pump

 

• Up to 50 mL/min flow rate

• 6,000 PSI maximum pressure

• In-line degasser

• 2 to 20 L buffer source bottles/carboys

Column Selector

 

• Ambient temperature

• Switchable for up to 6 columns

Detectors

 

• Pump pressure sensor

• Column temperature sensor

• Ultraviolet absorbance detector

• Deuterium lamp

• 190 to 600 nm wavelength range (up to two channels)

• 1 nm wavelength bandpass

• Up to 120 samples/second measurement (25 samples/second for dual channel) – Quartz flow cell 3 mm pathlength – 4 µL volume – 1,760 PSI maximum pressure

Fraction Collection

 

• Absorbance-based fraction collection methods

   

  • Absolute threshold
  • Derivative detection
  • Time based

• Up to 8x 96-well deep well plate fraction containers per batch

Sample Constraints

 

• 500 µL sample loop

• 500 µL autosampler syringe

• Up to 40x HPLC/CE vials or 2x SBS style deep well plates per batch

 

Semi-Preparative HPLC Viscometer

Differential Viscosity  

 

• Sensitivity (minimum detectable sample):  0.1 μg of 100 kDa polystyrene in THF at 1 mL/min, under typical GPC conditions

• Differential pressure noise: < 0.05 Pa

• Differential pressure range: 6.9 kPa, protected against overpressure

• Dynamic range: 135,000:1

• Linearity:  0.5% of full scale

• Differential pressure drift: < 2.5 Pa/hr

• Capillary bridge autobalance:  Thermal, leak-free mechanism

• Sample shear rate: 5000 Hz with water at 1 mL/min

Fluidics           

 

• Max. flow rate: Aqueous: 1.5 mL/min; THF: 3 mL/min

• Delay volume options: 16.2, 10.8 or 5.4 mL, with additional factory-installed option for 32.4 mL 1

• Max. permissible pressure inlet to outlet: 690 kPa

• Wetted Materials:  316 stainless steel, PTFE, zirconium silicate, Kalrez ® , Inconel, 26-1 Stainless Steel

Temperature Control 

 

• Range:  4 ºC to 70 ºC

• Stability:  <0.005°C

• Ambient Range:  0 ºC to 40 ºC, 20% to 70% RH

Electronics     

 

• Digital Output 20 bits @ 10 Hz

• Auxiliary I/O   

   

  • Inputs:  Autoinject contact closure, Alarm In, Analog In: 2 differential inputs, 18 bits resolution, range -10 V to +10 V
  • Outputs: Autoinject Retransmit, Alarm Out, Recycle Out

• On-board Computer  

   

  • Communications         Ethernet

• Front Panel Display: 7″ (178 mm), high-resolution, multi-touch display for viewing/setting signals, instrument settings and diagnostics.

• Dimensions:  58 cm (L) x 36 cm (W) x 18 cm (H)

 

ExperimentGasChromatography[]

Gas Chromatography System

Flow System

 

• He carrier gas available

• Inlets available:

   

  • Split/Splitless capillary column inlet
  • Multimode temperature-programmable capillary column inlet
  • Column head pressure up to 100 PSIG

Column Oven

 

• Temperature range: 30 to 450ºC

• Up to 120ºC/min temperature ramp

• Up to 2 capillary columns

• 0.53 mm maximum column inner diameter

Split/Splitless Capillary Inlet

 

• Suitable for capillary columns up to 0.53 mm inner diameter

• Split ratio up to 7,500:1 to prevent column overload

• Up to 1,250 mL/min total flow of He

• Inlet temperature 30 to 400ºC

Multimode Capillary Inlet

 

• Suitable for capillary columns up to 0.53 mm inner diameter

• Split ratio up to 7,500:1 to prevent column overload

• Up to 1,250 mL/min total flow of He

• Inlet temperature 30 to 400ºC

• Temperature programmable with ramp rates up to 900ºC/min

Mass Spectrometry

 

• Single quadrupole mass spectrometer

• 1.6 to 1,050 m/z mass range

• 100 to 200ºC quadrupole temperature

• 100 to 300ºC ion source temperature

• 100 to 350ºC transfer line temperature

• Scan speeds up to 12,500 m/z per second

• Electron ionization:

   

  • Dual filaments

• Chemical ionization:

   

  • Single filament
  • Methane reagent gas
  • Positive and negative mode

Flame Ionization Detector

 

• Responds to most organic compounds

• Minimum detectable level (for tridecane): < 1.2 pgC/s

• Linear dynamic range: > 10⁷ (± 10%)

• Up to 1,000 points/second data measurement

Autosampler Rail System

 

• Vortex mixing up to 2,000 RPM

• Sample incubation 30 to 200ºC

• Liquid, headspace, and solid-phase microextraction sample preparation available

• 0.01 to 100 µL injection volumes for liquid injection

• 25 to 2,500 µL injection volumes for headspace injection

• Headspace syringe heating 40 to 150ºC

ExperimentIonChromatography[]

Ion Chromatography System

Pumps

 

• Dual channel system

   

  • Anion Channel: Isocratic pump with pure water being primary solvent
  • Cation Channel: Quaternary pump

• 0.1 to 10 mL/min flow rate

• 5,000 PSI maximum pressure

• Static mixer

• In-line degasser

Eluent Generator

 

• Electrolytic eluent generation for anionic applications

• 0.1 to 100 mM concentration

Dynamically Regenerated Suppressors

 

• Constant voltage and constant current mode

• Maximum suppressor capabilities:

   

  • Anion channel: 50 μeq/min
  • Cation channel: 35 μeq/min

• 0.25 to 0.75 mL/min flow rate

Conductance Detectors

 

• Passivated 316 stainless steel electrode

• Maximum range up to 15,000 μS conductance measurement

• 0.7 µL flow cell volume

• One detector for each channel

• Shared detection oven with 15 to 60ºC (± 0.5ºC) incubation

Column Oven

 

• 10 to 70°C (± 0.1°C) incubation

• One analytical column for each channel

Sample Constraints

 

• 10 µL sample loops

• Up to 40x HPLC/CE vials or 2x SBS style deep well plates per batch

• Sample chamber temperature controlled from 4 to 60°C

Ion Chromatography System with Electrochemical Detector

Quaternary Pump

 

• Single channel system

• 0.1 to 10 mL/min flow rate

• 5,000 PSI maximum pressure

• Static mixer

• In-line degasser

Ultraviolet absorbance detector

 

• Tungsten and Deuterium lamp

• 190 to 900 nm wavelength range (up to four channels)

• 6 nm wavelength bandpass

• Up to 100 samples/second measurement

• PEEK flow cell

   

  • 10 mm path length
  • 11 μL flow cell volume

Electrochemical detector

 

• < 0.2 μL internal volume

• 100 PSI maximum pressure

• Gold, platinum, carbon and silver working electrodes

• pH-Ag/AgCl reference electrode

Column Oven

 

• 10 to 70°C (± 0.1°C) incubation

Sample Constraints

 

• 25 μL sample loop

• Up to 40x HPLC/CE vials or 2x SBS style deep well plates per batch

• Sample chamber temperature controlled from 4 to 60°C

ExperimentFPLC[]

Example applications include: Aqueous mobile phase, Protein purification, Biomolecule purification, Fast protein liquid chromatography, Medium-pressure chromatography, Liquid chromatography, Affinity chromatography

Fast Protein Liquid Chromatography System Medium and High Flow

System Pump & Mixer

 

• 0.001 to 25 mL/min flow rate (50 mL/min for column packing) for Avant 25

• 0.001 to 150 mL/min flow rate (300 mL/min for column packing) for Avant 150

• 20 MPa maximum pressure for Avant 25

• 5 MPa maximum pressure for Avant 150

• 1.4 L mixer volume

• ± 0.6% gradient composition accuracy for Avant 25

• ± 0.8% gradient composition accuracy for Avant 150

Detectors

 

• Pump pressure sensor

   

  • 0 to 20 MPa range
  • ± 0.02 MPa accuracy

• Temperature monitor

   

  • ± 1.5°C temperature accuracy

• Conductance sensor

   

  • 0.01 mS/cm to 1 S/cm conductance range
  • 22 µL flow cell volume

• Absorbance monitor

   

  • 190 to 700 nm wavelength range in increments of 1 nm wavelength
  • Up to 3 simultaneous wavelengths
  • UV flow cell

     

    • 0.5 mm, 2 mm, or 10 mm pathlengths
    • 1 μL, 2 μL, or 8 μL volumes
    • Noise < 0.08 mAU

• pH monitor

   

  • 0 to 14 range
  • ± 0.1 pH unit (within pH 2 to 12)

Fraction Collection

 

• Absorbance-based or conductivity-based fraction collection methods

   

  • Absolute threshold
  • Derivative detection
  • Time based
  • Start collection with sample application or within the gradient

• 50 mL container, 15 mL container, or 96-well deep well plate fraction collection

Sample Constraints

 

• Autosampler mode

   

  • 50 to 10,000 µL sample loop volume
  • 1,000 to 10,000 µL autosampler syringe
  • Up to 168x HPLC/CE vials or 2x SBS style deep well plate per batch
  • Up to 12 large sample vials (10 mL) for preparative system

• Flow injection mode

   

  • Up to 50 mL to 4 L sample container volumes
  • Up to 5 containers
  • Controllable sample introduction rate (up to 150 mL/min)

• Superloop mode

   

  • 10 mL loop volume
  • Up to 50 mL to 4 L sample container volumes
  • Up to 5 containers

ExperimentFlashChromatography[]

Flash Chromatography System

Binary Pump

 

• 500 to 200 mL/min flow rate

• 200 PSI maximum pressure

• Up to 20 L drum solvent sources

• Programmable gradient (± 1% accuracy)

Absorbance Detector

 

• Deuterium lamp

• 200 nm and 780 nm UV detection (5 nm bandpass)

• 0.6 µl flow cell (0.1 mm pathlength)

Fraction Collection

 

• 30x 50 mL vials per batch

• 70x 25 mL vials per batch

Column Constraints

 

• Luer lock connector pre-packed columns

• Up to 70 mm outer diamter flash columns

• Up to 16.5″ length flash columns

• ~5 to ~660 g of resin per column

Sample Constraints

 

• Up to 33 g of solid material for dry loading

• Up to 50 mL of liquid material for wet loading

ExperimentCapillaryGelElectrophoresisSDS[]

Protein Capillary Electrophoresis System

 

• 10 to 270 KDa molecular weight range

• Programmable sample electrokinetic injection and separation

   

  • Up to 20 steps
  • 0 to 6,500 V

• Sequential analysis on a single capillary

• Samples can be held at 4ºC, 10ºC, 15ºC, or ambient temperature while enqueued for injection

Detection

 

• UV absorbance: 220 nm

• Sensitivity: 0.3 µg/mL

Sample Constraints

 

• Minimum sample volume: 50 µL

• Up to 48 injections per batch

• Up to 12 injections per sample

• Up to 100 samples per cartridge

• Protein concentration: 0.2 to 2 mg/mL

• Salt concentration: up to 50 mM

ExperimentCapillaryIsoelectricFocusing[]

Protein Capillary Electrophoresis System

 

• 2.85 to 10.45 isoelectric point range

• Programmable focusing

   

  • Up to 20 steps
  • 0 to 5,000 V

• Modular ampholyte and internal standard composition

• Optional onboard mixing for sensitive samples

• Sequential analysis on a single capillary

• Samples can be held at 4ºC, 10ºC, 15ºC, or ambient temperature while enqueued for injection

Detection

 

• Whole capillary imaging using a CCD

• Sensitivity

   

  • UV absorbance: 3 µg/mL
  • Native fluorescence: 0.7 µg/mL

• Detectors

   

  • UV absorbance: 280 nm
  • Native fluorescence:

     

    • Excitation: 280 nm
    • Emission: 320 to 405 nm bandpass

Sample Constraints

 

• Minimum sample volume: 50 µL

• Up to 100 injections per batch

• Up to 12 injections per sample

• Up to 100 samples per cartridge

• Protein concentration: ~0.2 mg/mL

• Salt concentrations: up to 15 mM

ExperimentPAGE[]

ExperimentAgaroseGelElectrophoresis[]

Robotic Gel Electrophoresis System

Polyacrylamide Gel Electrophoresis

 

• Slab Gels

   

  • 4.5% and 10% TBE-Urea gels
  • 10% TBE gels
  • 7% and 12% Tris-Glycine gels
  • 5 mm width lanes
  • 3 mm depth gels

• Electrophoresis

   

  • 0 to 125 V
  • 1 to 100% duty cycle options

• Fluorescence Imaging

   

  • Post stained gels (stained with any custom solution)
  • Excitation filters

     

    • 470 nm (70 nm bandpass)
    • 625 nm (70 nm bandpass)

  • Emission filters

     

    • 540 nm (40 nm bandpass)
    • 678 nm (44 nm bandpass)

• Sample Constraints

   

  • Up to 20 µL of sample and loading buffer per lane
  • Up to 10 lanes per gel
  • Up to 4 gels per batch

Agarose Gel Electrophoresis

 

• Gels

   

  • Analytics (no sample recovery) or size selection (targeted recovery of analytes of particular sizes)
  • 0.5, 1.0, 1.5, 2.0, and 3.0% agarose
  • Independent lanes

• Electrophoresis

   

  • 100 V
  • 1 to 100% duty cycle options

• Fluorescence Imaging

   

  • Pre-stained markers ranging from 25 to 20,000 base pairs
  • Multiple in-run images
  • Excitation filters

     

    • 470 nm (70 nm bandpass)
    • 625 nm (70 nm bandpass)

  • Emission filters

     

    • 540 nm (40 nm bandpass)
    • 678 nm (44 nm bandpass)

• Sample Constraints (Size Selection)

   

  • Up to 50 µL of sample and loading buffer per lane
  • Up to 12 lanes per gel
  • Up to 4 gels per batch

• Sample Constraints (Analytical)

   

  • Up to 8 µL of sample and loading buffer per lane
  • Up to 23 lanes per gel
  • Up to 4 gels per batch

ExperimentWestern[]

ExperimentTotalProteinDetection[]

Example applications include: Capillary-based Analytical Assay, Gel Electrophoresis, Capillary Gel Electrophoresis, Total Protein Separation, Total Protein Visualization, Protein Size Characterization, Protein Abundance Comparison

Capillary Western Blot System

Capillary Gel Electrophoresis

 

• 2 to 440 kDa molecular weight range

• 0 to 500 V separation voltage

• UV capillary crosslinking for protein immobilization

Secondary Antibodies Available

 

• Secondary Antibodies Available

   

  • Goat Anti-Rabbit HRP
  • Goat Anti-Mouse HRP
  • Goat Anti-Human IgG HRP
  • Donkey Anti-Goat HRP

CCD Camera-Based Capillary Imaging

 

• 100 ms to 512 sec exposure times

• Up to picogram sensitivity (depending on the primary antibody sensitivity)

Capacity

 

• 13 or 25 capillaries per batch

• 5 to 8 µL of sample required per capillary

ExperimentCrossFlowFiltration[]

Example applications include: Ultrafiltration, Microfiltration, Diafiltration, Protein Purification, Tangential Flow Filtration, Buffer Exchange, Desalting

Tangential Flow Filtration System

 

• Volume range of 10 mL to 2 L

• Filtration area of 13 to 3,700 cm²

• Digitally controlled peristaltic pump

• Digital pressure monitor

• Automatic backpressure valve

• Digital conductivity monitors

• Absorbance monitor of 200 to 800 nm

• UV-Vis spectrometer

   

  • 200 to 1,100 nm wavelength range
  • CCD detector allows the entire wavelength
  • Sub-nanometer resolution
  • Up to 200 measurements/sec

 

ExperimentFieldFlowFractionation[]

Example applications include: Ultrafiltration, Microfiltration, Diafiltration, Protein Purification, Tangential Flow Filtration, Buffer Exchange, Desalting

Multiflow FFF System

Isocratic Pump

 

• Flow rate: 0.05 to 4 mL/min (micro), 0.05 to 9.95 mL-min (analytical), 0.2 to 40 mL/min (preparative)

• Maximum pressure: 40 MPa (micro), 40 MPa (analytical), 20 MPa (preparative)

Detectors

 

• Refractory Index detector: 8 µL flow cell volume

• UV/Vis detector: 4-channel diode array detector, wavelength 190-800nm, 10µL flow cell volume, 10 mm path length

   

  • Goat Anti-Rabbit HRP

• 21-angle Multi-angle Laser Light Scattering detector: using a 532nm laser at 50mW

Cartridges

 

• Modular spacers and membranes on FFF cartridges for various applicaitons: asymmetric flow, electrical asymmetric flow, and size exclusion, as well as disposable hollow-fiber cartridges

Autosampler

 

• Injection modes: full-loop, partial loop, and ul pickup

• Injection volume: 1 to 1,000 µL (up to 10 mL for preparative applications)

• Temperature control: 4 to 40ºC

• Sample shaking

Fraction collector

 

• Analytical and preparative (up to 10mg) scale applications

• Collection mode: targeted peaks and peak fractions

• Temperature control – 5-40Celsius

• Collection with needle directly to 2 ml vials, 96 well plates, or 10 ml preparative vials

Sample Constraints

 

• Particle size: up to 1Micrometer (Brownian mode), over 1Micrometer (Steric mode)

• Ideal for characterization of proteins, aggregates, liposomes, nanoparticles and various macromolecules

ExperimentDialysis[]

Example applications include: Fragile Proteins, Viscous Fluids, Polymer Gels, Equilibrium Dialysis, Small Molecule Binding to Proteins, Purification, Buffer Exchange, Desalting, Protein Purification

Static Dialysis

Beakers Available

 

• 250 mL, 600 mL, 1 L, and 2 L

• Incubation range 5 to 45°C (below ambient temperatures available for 250 mL beakers only)

• Buffer mixing available

Tube-Based Dialysis Kits

 

• 3.5k, 6 to 8k and 12 to 14k molecular weight cutoffs (MWCOs) available

• 10 µL to 20 mL sample volume range

• 4 to 60°C incubation range

• Up to 9 samples per batch

Dialysis Tubing

 

• 3.5k, 6 to 8k, and 12 to 14k MWCOs available

• 30 to 130 mL sample volume range

• Continuous buffer flow to increase separation efficiency

• 1 sample per batch

Equilibrium Dialysis

 

• 8 MWCO available

• 50 to 500 µL sample volume range

• 4 to 60°C incubation range

• Up to 48 samples per batch

Dynamic Dialysis

 

• 3.5k, 6k, and 12k MWCOs available

• 30 to 130 mL sample volume range

• Continuous buffer flow to increase separation efficiency

• > 90% protein recovery after dialysis

• 1 sample per batch

ExperimentThinLayerChromatography[]

High Performance TLC System (HPTLC)

Derivatization

 

• Automated micro-droplet spraying of chemical stains

• Application of aqueous or organic solutions

• Spot-to-spot relative standard deviation <5%

Application

 

• Up to 15 samples per HPTLC plate (20 x 10 cm)

• 5 to 6µm silica particle size

• Samples applied to plates as 8 mm bands for HPTLC

• Samples applied as spots for reaction monitoring

Development

 

• Gas phase can be actively controlled to ensure saturation of elution chamber

• Solvent front monitoring and automatic plate removal

• Development of TLC and HPTLC plate up to 20×20 cm

Visualization

 

• UV (254 and 366 nm) and white light illumination

• Visualize TLC and HPTLC plates up to 20×20 cm

• Rf evaluation and comparison within or between plates

• Semi-quantitative peak integration

ExperimentLiquidExtraction[]

Phase Separator

 

• Rapidly separates immiscible mixtures of organic and aqueous solutions via filtration

• Separation does not depend on whether the aqueous layer is on the top or bottom

• Up to 70 ML capacity per filtration

• Organic solvent flow rate up to 15 mL/min

Separatory Funnel

 

• Collects aqueous and organic layers of an immiscible mixture at a volume range of 10 mL to 1 L

Robotic Extraction

 

• Separate top layer from bottom layer given exact height of the top and bottom layer

• Control aspiration flow rate

ExperimentMagneticBeadSeparation[]

Example applications include: Magnetic Cell Separation, Protein Purification, Immunoprecipitation, Nucleic Acid Isolation

Magnetic Racks

Robotic Separation

 

• Performed by a Hamilton liquid handler with the Alpaqua Magnum FLX magnet

• Sample volume constraints

   

  • 96-well plate magnet: up to 2 mL

Manual Separation

 

• Performed by an operator with the DynaMag magnets

• Sample volume constraints

   

  • 96-well plate magnet: up to 0.2 mL
  • 2 mL tube magnet: up to 2 mL
  • 15 mL tube magnet: up to 15 mL
  • 50 mL tube magnet: up to 50 mL