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MASS SPECTROMETRY
ExperimentMassSpectrometry[]
ESI-QToF Mass Spectrometer
- High resolution, high mass accuracy mass spectrometer
- Conventional full-scan MS and MS/MS methods, data-dependent acquisition (DDA), as well as MS(E)
Electrospray Ionization (ESI) Ion Source
- 50 to 4,000 V ESI capillary voltage
- 1 to 200 V sampling cone voltage
- 60 to 18,000 L/hr cone gas flow rate
- 60 to 72,000 L/hr desolvation gas flow rate
Quadrupole, Time of Flight (QToF) Mass Analyzer
- 20 to 100,000 m/z ToF mass range
- 20 to 16,000 m/z quadrupole mass range
- 1 ppm RMS accuracy
- 22,500 FWHM mass resolution
- 2.46 kV (-1.8 kV grid) dual stage reflectron
- 25.2 µPa high vacuum pressure ToF Tube
- 350 V ion guide electrostatic lens
- Up to 30 spectra per second
Collision Cell
- 0 to 255 V collision cell voltage
- Argon collision cell gas
- 42.6 mPa high vacuum pressure collision cell
Sample Constraints
- Direct injection
- Sample volume range: 0.5 to 30 mL
- 2 mL HPLC vials or 30 mL reservoir bottles
- Maximum of 20 samples per batch
- Autosampler-aided direct infusion
- 75 µL sample loop for analytical flow, 10 µL for micro/nano flow
- Up to 48x HPLC/CE vials or 2x SBS style deep well plates per batch

ESI-QQQ Mass Spectrometer
- Standard triple quadrupole functions like MRM, precursor, neutral loss, and product ion scan
- QTRAP functionality allows for MS3 (MS/MS/MS)
- Enhanced scanning modes possible such as enhanced mass scans (EMS), enhanced resolution scans (ER), and enhanced product ion scans (EPI)
- 6 orders of magnitude dynamic range
Electrospray Ionization (ESI) Ion Source
- IonDrive Turbo V Ionization Source (NanoSource optional)
- 5 msec polarity switching in MRM and scheduled MRM modes
- Up to 4,500 V ion spray voltage
- Up to 750°C ion source temperature
Triple Quadrupole Mass Analyzer
- 20,000 Da/s scan speed and full scan linear ion trap sensitivity
- 5 to 2,000 m/z triple quadrupole mass range
- 50 to 2,000 m/z linear ion trap mass range
- Dual stage IonDrive QJet ion guide (non-funnel)
- Nitrogen collision cell for fragmentation
- Low, unit, and high resolution for Q1 and Q3

Echo MS System
- Highly reproducible sample analysis using Acoustic Droplet Ejection and Open Port Interface1
- Sample acquisition rates of up to 1 second per sample for a single analyte
- Electrospray ionization for broad compound coverage
- High sensitivity and quantitative robustness using the SCIEX Triple Quad 6500+ mass spectrometer
- Metabolic stability assay screening on the Echo MS system yielded up to 10x greater throughput compared to LC-MS/MS (Figure 1)

MALDI-ToF Mass Spectrometer
- Mass resolution up to 2,000 (at m/z of 1,500)
- Sensitivity down to 500 fmol BSA (66 kDa) with < 50 S/N
- Mass accuracy of < 30 ppm (internal calibration)
- Negative or positive ion modes
- Up to 500 kDa mass range
Laser Ionization Source
- 337 nm wavelength
- 60 Hz laser frequency
- 3 ns pulse width
- 9 mW power
- 1 to 1,000 shots per well
Time of Flight (ToF) Mass Analyzer
- 75 cm length ToF tube
- 2 µBar high vacuum
- Up to 20 kV accelerating voltage
- Up to 20 kV grid voltage
- 0.05 to 10 kV electrostatic lens voltage
- Up to 5,000 ns delay time
Triple Quadrupole Mass Analyzer
- Up to 2 µL of sample per well
- Up to 2 µL of calibrant per well
- 96 wells per experiment

ExperimentLCMS[]
Liquid Chromatography Mass Spectrometer
Liquid Chromatography
- Analytical HPLC
- 0.1 to 2.2 mL/min flow rate
- 0.01 inch inner diameter PEEK system tubing
- Up to 7,000 psi operating pressure
- In-line degasser
- Photo diode array detector
- 190 to 500 nm absorbance
- 0.5 μL volume
- 5 to 90°C (± 0.1°C) column compartment temperature range
- Up to two columns connected in series up to 300 mm maximum in length (15 mm maximum outer diameter)
- Micro/Nano HPLC
- Performs using micro (1 to 100 µLimin) to nano (10 to 1,000 nLimin) fow rate
- Optimized for columns from 75 µm to 4 6 mm internal diameter
- Superior chromatographic resolution with sub-2-µm column particle technology
- Optimal for applications requiring high sensitivity analysis with limited sample volumes
- Up to 15,000 PSI operating pressure
- Integrated vacuum degassing
- 5 to 90°C (± 0.1°C) column compartment temperature range
- Single column up to 250 mm maximum length (15 mm maximum outer diameter)
- High pressure mixing, binary gradient
- Trap valve manager with two six-port two-position valves
- Up to four solvents, in combinations of two (A1 or A2, and B1 or B2)
Mass Spectrometry
- QToF
- High resolution, high mass accuracy mass spectrometer
- Conventional full-scan MS and MS/MS methods, data-dependent acquisition (DDA), as well as MS(E) acquisition modes
- 20 to 100,000 m/z ToF mass range
- 20 to 16,000 m/z quadrupole mass range
- 22,500 FWHM mass resolution
- 1 ppm RMS accuracy
- Up to 30 spectra per second
- Analytical QQQ
- Performs standard triple quadrupole functions like MRM, precursor, neutral loss, and product ion scan
- Enhanced scanning modes possible such as MS3, enhanced mass scans (EMS), enhanced resolution scans (ER), and enhanced product ion scans (EPI)
- 5 to 2,000 m/z triple quadrupole mass range
- 50 to 2,000 m/z linear ion trap mass range
- 6 orders of magnitude dynamic range
- 5 msec polarity switching in MRM and scheduled MRM modes
- 20,000 Da/s scan speed
- Full scan linear ion trap sensitivity
Sample Constraints
- 75 µL sample loop for analytical LC, 10 µL sample loop for micro/nano LC
- Sample chamber temperature controlled from 5 to 40°C (± 3°C)
- Up to 48x HPLC/CE vials or 2x SBS style deep well plates per batch




ExperimentGCMS[]
ExperimentGasChromatography[]
Gas Chromatography Mass Spectrometer
Flow System
- He carrier gas available
- Inlets available:
- Split/Splitless capillary column inlet
- Multimode temperature-programmable capillary column inlet
- Column head pressure up to 100 PSIG
Column Oven
- Temperature range: 30 to 450ºC
- Up to 120ºC/min temperature ramp
- Up to 2 capillary columns
- 0.53 mm maximum column inner diameter
Split/Splitless Capillary Inlet
- Suitable for capillary columns up to 0.53 mm inner diameter
- Split ratio up to 7,500:1 to prevent column overload
- Up to 1,250 mL/min total flow of He
- Inlet temperature 30 to 400ºC
Multimode Capillary Inlet
- Suitable for capillary columns up to 0.53 mm inner diameter
- Split ratio up to 7,500:1 to prevent column overload
- Up to 1,250 mL/min total flow of He
- Inlet temperature 30 to 400ºC
- Temperature programmable with ramp rates up to 900ºC/min
Mass Spectrometry
- Single quadrupole mass spectrometer
- 1.6 to 1,050 m/z mass range
- 100 to 200ºC quadrupole temperature
- 100 to 300ºC ion source temperature
- 100 to 350ºC transfer line temperature
- Scan speeds up to 12,500 m/z per second
- Electron ionization:
- Dual filaments
- Chemical ionization:
- Single filament
- Methane reagent gas
- Positive and negative mode
Autosampler Rail System
- Vortex mixing up to 2,000 RPM
- Sample incubation 30 to 200ºC
- Liquid, headspace, and solid-phase microextraction sample preparation available
- 0.01 to 100 µL injection volumes for liquid injection
- 25 to 2,500 µL injection volumes for headspace injection
- Headspace syringe heating 40 to 150ºC



ExperimentCapillaryElectrophoresis[]
ExperimentCEMS[]
Capillary Electrophoresis Mass Spectrometer
CESI 8000 Plus ESI-MS System
- Sample Trays: 2 x 48 CESI vials / 0.3 mL microvials
- Buffer Tray: 2 x 36 CESI vials
- Pressure Delivery Range: -5 to +100 PSI
- Required Sample Volume: 5 µL when using NanoVias
- Zero Dead Volume
- Multi-Detection Capability: UV/VIS, PDA, or LIF detection can be selected in stand-alone CE mode
Waters Xevo TQ-S MS
- Flow Rate: up to 2 mL/min
- Mass Range: 2 to 2,048 m/z
- Scan Speed: up to 10,000 Da/s
- Acquisition Modes: Full scan MS, product ion scan, precursor ion scan, constant neutral loss, selected ion recording, multiple reaction monitoring (MRM)
- Interchangeable Ion Sources: ionKey/MS, APGC, APPI, APCI, ESI, ASAP